MOLEKULARNA DETEKCIJA FUSARIJUMSKIH INFEKCIJA U PŠENICI - MERA PROCENE KVALITETA

  • Sorina Popescu Banat University of Agricultural Aciences and Veterinary Medicine "King Michael I" from Timisoara
  • Oana Maria Boldura Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara (BUASVMT)
  • Aurica Borozan Faculty of Horticulture and Forestry, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara (BUASVMT)
  • Emilian Madosa Faculty of Horticulture and Forestry, Banat University of Agricultural Sciences and Veterinary Medicine “King Michael I of Romania” from Timisoara (BUASVMT)
DOI: https://doi.org/10.5937/jpea26-36835

Sažetak


Cilj ovog rada je bio da se proceni 50 uzoraka pšenice prikupljenih sa različitih lokacija u zapadnoj Rumuniji na osnovu mikrobioloških, molekularnih i toksikogenih testova kako bi se utvrdila njihova korelacija kada su analizirane vrste roda Fusarium. Za procenu rane gljivične infekcije korišćeno je prisustvo toksina utvrđeno biohemijskom ELISA (Enzime-Linked Immunosorbent Assai), DNK analizom zasnovanom na PCR (Polimerase Chain Reaction), pa čak i tačna identifikacija vrsta korišćenjem specifičnog sekvenciranja gena. S obzirom na to da je u Rumuniji prevalenca infekcija Fusarium graminearum i Fusarium proliferatum najvažnija, može se reći da skrining sa prajmerima specifičnim za gljivične vrste obezbeđuje preliminarni test za identifikaciju gljivične infekcije pre perforacije.

Reference

REFERENCES



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Objavljeno
2022/04/15
Broj časopisa
Vol. 26 Br. 1 (2022): Journal on Processing and Energy in Agriculture
Rubrika
Članci