PRETREATMENT OF LIGNOCELLULOSIC BIOMASS WITH AUTOCHTHONOUS SFUNGI FROM SERBIA
Keywords:
autochthonous fungi, lignocellulose, laccase, manganese peroxidase, cellulase, xylanase
Abstract
This research examined the potential use of isolated Serbian autochthonous fungi in lignocellulosic biomass pretreatment. Among 12 isolated fungi, the isolates identified as Trametes hirsuta F13 and Stereum gausapatum F28 stood out as ligninolytic enzyme producers and were selected for potential use in the pretreatment of a waste lignocellulosic biomass. An isolate identified as Myrmaecium fulvopruinatum F14 showed high hydrolytic activity, but negligible ligninolytic activity, and it was selected as a potential producer of important industrial hydrolytic enzymes.
Further, the breakdown of lignocellulosic waste, beechwood sawdust, by T. hirsuta F13 and S. gausapatum F28 was examined. Both isolates efficiently degraded biomass, but T. hirsuta F13 exhibited greater selectivity (selectivity coefficient of 1.7) than S. gausapatum F28 (1.1). The isolate F13 was considered a better candidate for the pretreatment, and it was selected for further analysis which involved the use of molasses stillage as a supplement to improve the pretreatment.
References
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Templeton, D., and Ehrman, T. (1995). Determination of acid-insoluble lignin in biomass, Laboratory Analytical Procedure No. 003. National Renewable Energy Laboratory, Golden, CO.
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Zhang, J., Zhou, H., Liu, D., and Zhao, X. (2020). Chapter 2—Pretreatment of lignocellulosic biomass for efficient enzymatic saccharification of cellulose. In A. Yousuf, D. Pirozzi, and F. Sannino (Eds.), Lignocellulosic Biomass to Liquid Biofuels (pp. 17–65). Academic Press.
Archibald, F. S. (1992). A new assay for lignin-type peroxidases employing the dye azure B. Applied and Environmental Microbiology, 58(9), 3110–3116.
Arora, D. S., and Gill, P. K. (2005). Production of ligninolytic enzymes by Phlebia floridensis. World Journal of Microbiology and Biotechnology, 21(6), 1021–1028.
Champreda, V., Mhuantong, W., Lekakarn, H., Bunterngsook, B., Kanokratana, P., Zhao, X.-Q., Zhang, F., Inoue, H., Fujii, T., and Eurwilaichitr, L. (2019). Designing cellulolytic enzyme systems for biorefinery: From nature to application. Journal of Bioscience and Bioengineering, 128(6), 637–654.
Choudhary, M., Sharma, P. C., Jat, H. S., Nehra, V., McDonald, A. J., and Garg, N. (2016). Crop residue degradation by fungi isolated from conservation agriculture fields under rice–wheat system of North-West India. International Journal of Recycling of Organic Waste in Agriculture, 5(4), 349–360.
Ehrman, T. (1996). Determination of acid-soluble lignin in biomass, Laboratory Analytical Procedure No. 004. National Renewable Energy Laboratory, Golden, CO.
Gauna, A., Larran, A. S., Perotti, V. E., Feldman, S. R., and Permingeat, H. R. (2018). Fungal pretreatments improve the efficiency of saccharification of Panicum prionitis Ness biomass. Biofuels, 0(0), 1–7.
Isroi, Millati, R., Syamsiah, S., Niklasson, C., Cahyanto, M. N., Ludquist, K., and Taherzadeh, M. J. (2011). Biological pretreatment of lignocelluloses with white-rot fungi and its applications: A review. BioResources, 6(4), 5224–5259.
Jović, J., Buntić, A., Radovanović, N., Petrović, B., and Mojović, L. (2018). Lignin-degrading abilities of novel autochthonous fungal isolates Trametes hirsuta F13 and Stereum gausapatum F28. Food Technology and Biotechnology, 56(3), 354–365.
Jović, J., Hao, J., Kocić-Tanackov, S., and Mojović, L. (2020). Improvement of lignocellulosic biomass conversion by optimization of fungal ligninolytic enzyme activity and molasses stillage supplementation. Biomass Conversion and Biorefinery.
Kuwahara, M., Glenn, J. K., Morgan, M. A., and Gold, M. H. (1984). Separation and characterization of two extracelluar H2O2-dependent oxidases from ligninolytic cultures of Phanerochaete chrysosporium. FEBS Letters, 169(2), 247–250.
Mielenz, J. R. (2020). Chapter 27—Small-scale approaches for evaluating biomass bioconversion for fuels and chemicals. In A. Dahiya (Ed.), Bioenergy (Second Edition) (pp. 545–571). Academic Press.
Miller, G. L. (2002, May 1). Use of dinitrosalicylic acid reagent for determination of reducing sugar (world) [Research-article]. American Chemical Society.
Nair, A. S., and Sivakumar, N. (2020). Chapter 16—Recent advancements in pretreatment technologies of biomass to produce bioenergy. In V. K. Gupta, H. Treichel, R. C. Kuhad, and S. Rodriguez-Cout (Eds.), Recent Developments in Bioenergy Research (pp. 311–324). Elsevier.
Reina, R., Kellner, H., Hess, J., Jehmlich, N., García-Romera, I., Aranda, E., Hofrichter, M., and Liers, C. (2019). Genome and secretome of Chondrostereum purpureum correspond to saprotrophic and phytopathogenic life styles. PLOS ONE, 14(3), e0212769.
Sluiter, A., Hames, B., Hyman, D., Payne, C., Ruiz, R., Scarlata, C., Sluiter, J., Templeton, D., and Wolfe, J. (2008). Determination of total solids in biomass and total dissolved solids in liquid process samples: Laboratory analytical procedure (LAP): Issue Date, 3/31/2008. National Renewable Energy Laboratory, Golden, Colo.
Templeton, D., and Ehrman, T. (1995). Determination of acid-insoluble lignin in biomass, Laboratory Analytical Procedure No. 003. National Renewable Energy Laboratory, Golden, CO.
Vučurović, V. M., Puškaš, V. S., Miljić, U. D., Đuran, J. J., and Filipović, J. S. (2019). Bioethanol production from sugar beet thick juice by Saccharomyces cerevisiae immobilized in alginate-maize stem ground tissue beads. Journal on Processing and Energy in Agriculture, 23(4), 167–169.
Zhang, J., Zhou, H., Liu, D., and Zhao, X. (2020). Chapter 2—Pretreatment of lignocellulosic biomass for efficient enzymatic saccharification of cellulose. In A. Yousuf, D. Pirozzi, and F. Sannino (Eds.), Lignocellulosic Biomass to Liquid Biofuels (pp. 17–65). Academic Press.
Published
2021/05/31
Section
Papers