QUALITY OF ALFALFA SEEDS FROM DIFFERENT REGIONS OF SERBIA DURING STORAGE UP TO 42 MONTHS
Keywords:
alfalfa, quality, seed, storage
Abstract
This study presents the findings from assessing the quality of alfalfa seeds collected from three Serbian regions: in each region, batches of seeds from six places were analyzed. The following criteria were investigated: the proportion of dormant seeds, the germinated seed content, the proportion of dead seeds, and the percentage of aberrant seedlings. Seed quality was assessed after six, eighteen, thirty, and forty-two months of storage. The highest seed quality was obtained after thirty and eighteen months of seed storage (91% and 89% of germination, respectively). During the 42-month storage period, the percentage of dead seeds (8%) and abnormal seedlings (7%) increased, while germination declined to 84%. In a storage period of six months, the participation of dormant seeds of 21% affected the germination rate, which was 77%, regardless of the low proportion of dead seeds (1%) and abnormal seedlings (2%).
References
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Baskin J.M., Baskin, C.C. (2021). The great diversity in kinds of seed dormancy: A revision of the Nikolaeva–Baskin classification system for primary seed dormancy. Seed Sci. Res., 31: 249–277
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Corbineau, F. (2024). The Effects of Storage Conditions on Seed Deterioration and Ageing: How to Improve Seed Longevity. Seeds, 3: 56–75.
Cuena Lombraña A., Dessì L., Podda L., Fois M., Luna B., Porceddu M., Bacchetta, G. (2024). The Effect of Heat Shock on Seed Dormancy Release and Germination in Two Rare and Endangered Astragalus L. Species (Fabaceae). Plants, 13, 484. https://doi.org/10.3390/plants13040484
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Fairey, D.F., Lefkovitch, L.P. (1991). Hard seed content of alfalfa grow in Canada. Can. Jour. of Plant Scii., 71: 437-444.
Frischie S, Miller A.L, Pedrini S, Kildisheva O.A (2020). Ensuring seed quality in ecological restoration: native seed cleaning and testing. Rest. Ecol., 28: 239-248
Galussi, A.A., Argüello, J.A., Cerana, M.M., Maximino, M., Moya, M.E. (2016). Anatomical and chemical characteristics of the seed coat of Medicago sativa L. (alfalfa) cv. Baralfa 85 seeds and their association with seed dormancy. Phyton, Inter. Jour. of Expe. Botany, 84(1): 163-175.
Harrison R.J, Howieson J.G, Yates R.J, Nutt B.J. (2021). Long-term storage of forage legumes greatly alters the hard seed breakdown pattern in situ. Grass Forage Sci., 76: 72–81.
Jaganathan, G.K., Berry K. (2023) Climate Change during Cretaceous/Paleogene as a Driving Force for the Evolutionary Radiation of Physical Dormancy in Fabaceae. Seeds, 2: 309-317.
Kimura, E., Islam A. M. (2012). Seed scarification methods and their use in forage legumes. Rese. Jour. of Seed Sci., 5: 38-50.
Knežević J., Tomić D., Jovanović D., Tmušić N., Štrbanović R., Poštić D., Stanisavljević R. (2019): Seed Quality of Oilseed Rape Varieties with Different Size and Colors After Three and Fifteen Months Storage. Tarım Bilimleri Dergisi-Jour. of Agric. Scie. 25: 449–458.
Nadarajan J., Walters C., Pritchard H.W., Ballesteros D., Colville L. (2023). Seed longevity—The evolution of knowledge and a conceptual framework. Plants, 12, 471.
Nagel M and A Börner (2010). The longevity of crop seeds stored under ambient conditions. Seed Scie. Res., 20(1): 1-12.
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Rawlins J.K., Roundy B.A., Davis S.M., Egget D. (2012). Predicting germination in semi-arid wildland seedbeds. I. Thermal germination Models. Environ. Exp. Bot., 76: 60–67.
seed germination and viability of some medicinal plants. Afr. J. Agric. Res. 20
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Stanisavljević R., Poštić D., Milenković J., Đokić D., Tabaković M., Jovanović S., Štrbanović R. (2018). Possibilities for improving the quality of alfalfa seed by applying temperature treatments before sowing. Journal on Proc. and Ene. in Agric., 22(2):76-9.
Stanisavljevic R., Poštić D., Štrbanović R., Tabaković M., Jovanović S., Milenković J., Đokić D., Terzić D. (2020): Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses. Trop. Grass.-Forrajes Tropicales 8(2): 125-132.
Tiwari R.K.S. and Das K. (2014). Impact of differential storage conditions on seed germination and viability of some medicinal plants. African Jour. Agric. Res., 9 (20): 1578- 1585.
Xie J., Li Y., Jiang G., Sun H., Liu X., Han L. (2023). Seed color represents salt resistance of alfalfa seeds (Medicago sativa L.): Based on the analysis of germination characteristics, seedling growth and seed traits. Front Plant Sci. 2023 doi: 10.3389/fpls.2023.1104948.
Zimmermann L.R., Galussi A.A., Martinelli A.H.M., Fernández A.P., García A.H., Pitter J.R., Zecchin A.R. and Dechanzi D.I. (1998). Hard seed viability in Medicago sativa L., Lotus corniculatus L., Trifolium repens L., T. pratense L. and Melilotus alba Med. Seed Sci. Technol., 26: 271-273.
and seed sprouting. Agronomy Monograph, 29: 961-983
Baskin J.M., Baskin, C.C. (2021). The great diversity in kinds of seed dormancy: A revision of the Nikolaeva–Baskin classification system for primary seed dormancy. Seed Sci. Res., 31: 249–277
Bass L.N., Gunn C.R., Hesterman O.B. and Roos E.E. (1988). Seed physiology, seedling performance and seed sprouting. Agronomy Monograph 29: 961-983
Bass, L.N., Gunn, C.R., Hesterman, O.B. and Roos, E.E. (1988). Seed physiology, seedling performance
Bukvic Z., Pacek D., Popovic S., Rutar R., Stjepanovic M. Effect of temperature on germination and hard alfalfa seed. In : Delgado I. (ed.), Lloveras J. (ed.). Quality in lucerne and medics for animal production . Zaragoza : CIHEAM, 2001. p. 137-139 (Options Méditerranéennes : Série A. Séminaires Méditerranéens; n. 45)
Corbineau, F. (2024). The Effects of Storage Conditions on Seed Deterioration and Ageing: How to Improve Seed Longevity. Seeds, 3: 56–75.
Cuena Lombraña A., Dessì L., Podda L., Fois M., Luna B., Porceddu M., Bacchetta, G. (2024). The Effect of Heat Shock on Seed Dormancy Release and Germination in Two Rare and Endangered Astragalus L. Species (Fabaceae). Plants, 13, 484. https://doi.org/10.3390/plants13040484
Čupić T., Popović S., Grljušić S., Tucak M., Andrić L., Šimić B. (2005). Effect of storage time on alfalfa seed quality. Jour. of Cen. Europ. Agric., 6: 65- 68.
Fairey, D.F., Lefkovitch, L.P. (1991). Hard seed content of alfalfa grow in Canada. Can. Jour. of Plant Scii., 71: 437-444.
Frischie S, Miller A.L, Pedrini S, Kildisheva O.A (2020). Ensuring seed quality in ecological restoration: native seed cleaning and testing. Rest. Ecol., 28: 239-248
Galussi, A.A., Argüello, J.A., Cerana, M.M., Maximino, M., Moya, M.E. (2016). Anatomical and chemical characteristics of the seed coat of Medicago sativa L. (alfalfa) cv. Baralfa 85 seeds and their association with seed dormancy. Phyton, Inter. Jour. of Expe. Botany, 84(1): 163-175.
Harrison R.J, Howieson J.G, Yates R.J, Nutt B.J. (2021). Long-term storage of forage legumes greatly alters the hard seed breakdown pattern in situ. Grass Forage Sci., 76: 72–81.
Jaganathan, G.K., Berry K. (2023) Climate Change during Cretaceous/Paleogene as a Driving Force for the Evolutionary Radiation of Physical Dormancy in Fabaceae. Seeds, 2: 309-317.
Kimura, E., Islam A. M. (2012). Seed scarification methods and their use in forage legumes. Rese. Jour. of Seed Sci., 5: 38-50.
Knežević J., Tomić D., Jovanović D., Tmušić N., Štrbanović R., Poštić D., Stanisavljević R. (2019): Seed Quality of Oilseed Rape Varieties with Different Size and Colors After Three and Fifteen Months Storage. Tarım Bilimleri Dergisi-Jour. of Agric. Scie. 25: 449–458.
Nadarajan J., Walters C., Pritchard H.W., Ballesteros D., Colville L. (2023). Seed longevity—The evolution of knowledge and a conceptual framework. Plants, 12, 471.
Nagel M and A Börner (2010). The longevity of crop seeds stored under ambient conditions. Seed Scie. Res., 20(1): 1-12.
Official gazette SFRJ, No. 47/87. Rule on the quality of seeds of agricultural plants. 1987 and supplement to 34/2013)
Rawlins J.K., Roundy B.A., Davis S.M., Egget D. (2012). Predicting germination in semi-arid wildland seedbeds. I. Thermal germination Models. Environ. Exp. Bot., 76: 60–67.
seed germination and viability of some medicinal plants. Afr. J. Agric. Res. 20
Snedecor G.W., Cochran W.G. (1980). Statistical Methods. 7th ed. The Iowa State University Press, Ames, Iowa.
Stanisavljević R., Poštić D., Milenković J., Đokić D., Tabaković M., Jovanović S., Štrbanović R. (2018). Possibilities for improving the quality of alfalfa seed by applying temperature treatments before sowing. Journal on Proc. and Ene. in Agric., 22(2):76-9.
Stanisavljevic R., Poštić D., Štrbanović R., Tabaković M., Jovanović S., Milenković J., Đokić D., Terzić D. (2020): Effect of seed storage on seed germination and seedling quality of Festulolium in comparison with related forage grasses. Trop. Grass.-Forrajes Tropicales 8(2): 125-132.
Tiwari R.K.S. and Das K. (2014). Impact of differential storage conditions on seed germination and viability of some medicinal plants. African Jour. Agric. Res., 9 (20): 1578- 1585.
Xie J., Li Y., Jiang G., Sun H., Liu X., Han L. (2023). Seed color represents salt resistance of alfalfa seeds (Medicago sativa L.): Based on the analysis of germination characteristics, seedling growth and seed traits. Front Plant Sci. 2023 doi: 10.3389/fpls.2023.1104948.
Zimmermann L.R., Galussi A.A., Martinelli A.H.M., Fernández A.P., García A.H., Pitter J.R., Zecchin A.R. and Dechanzi D.I. (1998). Hard seed viability in Medicago sativa L., Lotus corniculatus L., Trifolium repens L., T. pratense L. and Melilotus alba Med. Seed Sci. Technol., 26: 271-273.
Published
2024/04/04
Section
Papers