MICROMORPHOLOGICAL FEATURES OF MASTOCYTES IN THE TRIGEMINAL AND HUMAN SYMPATHETIC SUPERIOR CERVICAL GANGLIONS

Mila Ćetković; Aleksandra Milosavljević; Jelena Boljanović; Darko Laketić; Marko Simić; Nikola Bogosavljević; Aleksandar Mirčić; MILAN Milisavljević

doi:10.5937/medi58-56590

Mila Ćetković Institute of Histology and Embryology
Aleksandra Milosavljević Institute of Histology and Embryology, Faculty of Medicine, University of Belgrade, Belgrade
Jelena Boljanović Laboratory for Vascular Morphology, Institute of Anatomy, Faculty of Medicine, University of Belgrade, Belgrade
Darko Laketić Laboratory for Vascular Morphology, Institute of Anatomy, Faculty of Medicine, University of Belgrade, Belgrade
Marko Simić Clinic for Orthopaedic Surgery and Traumatology, University Clinical Center of Serbia, Faculty of Medicine, University of Belgrade, Belgrade
Nikola Bogosavljević Institute for Orthopedic Surgery “Banjica”, Faculty of Medicine, University of Belgrade, Belgrade
Aleksandar Mirčić Institute of Histology and Embryology, Faculty of Medicine, University of Belgrade, Belgrade
MILAN Milisavljević Laboratory for Vascular Morphology, Institute of Anatomy, Faculty of Medicine, University of Belgrade, Belgrade

DOI: https://doi.org/10.5937/medi58-56590

Keywords: micromorphological analysis, mastocytes, mastocytes density, sympathetic superior cervical ganglion, trigeminal ganglion

Abstract

Introduction: Mastocytes (Ms) are usually localized close to microvessels and terminals of the nerves innervating meninges and visceral organs. The granules of Ms contain numerous mediators that affect nerve cells and modify their reactions.

Aim: Our study was conducted with the aim to analyze the presence and localization of Ms, and mastocytes density (MsD) in three segments of the human trigeminal ganglions (TGs): ophthalmic, maxillary and mandibular, as well as in two parts: upper and lower, of the sympathetic superior cervical ganglions (SSCGs), and to establish the correlation between the studied parameters.

Material and Methods: Five TGs and five SSCGs of adult individuals were processed for this histochemical and immunohistochemical analysis. The sections are serially sliced and stained with hematoxylin and eosin and the trichrome method of Masson, as well as for the mastocyte tryptase immunostaining.

Results: The average number of tryptase-positive (Tryp+) Ms in the marked fields on the microscope, the MsD, was 1.4 in the ophthalmic parts, 1.3 in the maxillary parts, and 1.5 in the mandibular parts of TGs. The average number of Tryp+ Ms in fields on the microscope, the MsD, was 4.35 in the upper segments, and 4.45 in the lower segments of SSCGs. The distributions of Ms in three parts of the human TGs and two segments of the SSCGs were evenly distributed with no special morphological peculiarities. This is the first micromorphological analysis of the Tryp+ Ms and MsD in human TGs and SSCGs.

Conclusion: We found statistically significant differences in MsD of Tryp+ Ms between TG and SSCG (p = 0.000). MsD in two parts of the SSCG was significantly higher than MsD in three parts of TG.

MIKROMORFOLOŠKE KARAKTERISTIKE MASTOCITA U TIGEMINALNIM I HUMANIM SIMPATIČKIM GORNJIM VRATNIM GANGLIONIMA

Abstract