The activity of native Bacillus subtilis strains in control of green mould disease of oyster mushroom (Pleurotus spp.)

  • Ivana Potočnik Institute of Pesticides and Environmental Protection, Banatska 31b, 11080 Belgrade
  • Svetlana Milijašević-Marčić Institute of Pesticides and Environmental Protection, Banatska 31b, 11080 Belgrade
  • Olja Stanojević University of Belgrade, Faculty of Biology, Microbiology Department, Studentski trg 16, 11000 Belgrade
  • Tanja Berić University of Belgrade, Faculty of Biology, Microbiology Department, Studentski trg 16, 11000 Belgrade
  • Slaviša Stanković University of Belgrade, Faculty of Biology, Microbiology Department, Studentski trg 16, 11000 Belgrade
  • László Kredics University of Szeged, Faculty of Science and Informatics, Department of Microbiology, H-6726 Szeged, Közép fasor 52
  • Lóránt Hatvani University of Szeged, Faculty of Science and Informatics, Department of Microbiology, H-6726 Szeged, Közép fasor 52
Keywords: oyster mushrooms, green mould disease, Bacillus subtilis, antagonistic activity, biocontrol,

Abstract


The study aimed to isolate potential biocontrol agents from mushroom substrate that could serve as an alternative to toxic chemicals commonly used for disease control in mushroom production. The antagonistic potential of ten native Bacillus subtilis strains against the causal agents of green mould disease of oyster mushroom, Trichoderma pleuroti and Trichoderma pleuroticola, was evaluated. The antagonistic potential of Bacillus spp. strains was quantified in vitro based on dual cultivation with the pathogen. Growth inhibition of T. pleuroti ranged from 54.44% to 62.22% and no significant differences in antagonistic activity were found between the tested B. subtilis strains. Inhibition of T. pleuroticola was slightly higher, ranging from 55.56% to 69.62% and B. subtilis strain B-358 induced the highest growth inhibition. This research confirmed mushroom substrate to be a good source of antagonistic microorganisms with potentials for use in biological control of green mould in oyster mushroom production.

 

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Published
2019/12/30
Issue
Vol. 34 No. 2 (2019): Pesticides and Phytomedicine / Pesticidi i fitomedicina
Section
Original Scientific Paper

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