Effects of fungicides and biofungicides on Rhizoctonia solani, a pathogen of pepper

  • Milica Mihajlović Institut za pesticide i zaštitu životne sredine
  • Emil Rekanović Institute of Pesticides and Environmental Protection, Banatska 31b, Belgrade
  • Jovana Hrustić Institute of Pesticides and Environmental Protection, Banatska 31b, Belgrade
  • Mila Grahovac University of Novi Sad, Faculty of Agriculture, Trg Dositeja Obradovića 8, Novi Sad
  • Marija Stevanović Institute of Pesticides and Environmental Protection, Banatska 31b, Belgrade
  • Brankica Tanović Institute of Pesticides and Environmental Protection, Banatska 31b, Belgrade
Keywords: Rhizoctonia solani, pepper, fungicides, biofungicides, sensitivity, efficacy

Abstract


In vitro and in vivo sensitivity of Rhizoctonia solani to several commercial fungicides and biofungicides was studied. An isolate of R. solani, derived from diseased pepper plants originating from a greenhouse in Knjaževac, Serbia, was used. The highest effi cacy in R. solani control under greenhouse conditions was achieved by iprodione (95.80%, compared to control), although diff erences in the eff ectiveness of iprodione, tea tree oil, azoxystrobin and thiophanate-methyl were not statistically signifi cant. The isolate was sensitive to all tested products in vitro. The obtained EC50s were: 0.43 mg/l for iprodione, 1.84 mg/l for thiophanate-methyl, 13.84 mg/l for prochloraz, 430.37 mg/l for fluopyram, 596.60 mg/l for azoxystrobin, and 496.79 mg/l for tea tree oil.

 

References

Bartlett, D.W., Clough, J.M., Godwin, J.R., Hall, A.A., Hamer, M., & Parr-Dobrzanski, B. (2002). The strobilurin fungicides. Pest Management Science, 58(7), 649-662.

Burt, S. (2004). Essential oils: their antibacterial properties and potential applications in foods—a review. International Journal of Food Microbiology, 94(3), 223-253.

Carson, C.F., Hammer, K.A., & Riley, T.V. (2006). Melaleuca alternifolia (tea tree) oil: a review of antimicrobial and other medicinal properties. Clinical Microbiology Reviews, 19(1), 50-62.

Coa, L., Qiu, Z., You, J., Tan, H., & Zhou, S. (2004). Isolation and characterization of endophytic Streptomyces strains from surface-sterilized tomato (Lycopersicon esculentum) roots. Letters in Applied Microbiology, 39(5), 425-430.

Csinos, A.S., & Stephenson, M.G. (1999). Evaluation of fungicides and tobacco cultivar resistance to Rhizoctonia solani incited target spot, damping off and sore shin. Crop Protection, 18(6), 373-377.

D’ercole, N., Nipoti, P., Di Pillo, L., & Gavina, F. (2000). In vitro and in vivo tests of Trichoderma spp. as a biocontrol agent of Verticillium dahliae Kleb. in eggplants. In: E. C. Tjamos, R. C. Rowe, J. B. Heale & D. R. Fravel (eds.), Advances in Verticillium: Research and Disease Management (pp 260-263). St. Paul, MN, USA: APS Press.

Dhingra, O.D., &, Sinclair J.B. (1995). Basic plant pathology methods (2nd ed.). Boca Raton, FL, USA: CRC Press.

EPPO (1997). Guidelines for the efficacy evaluation of plant protection products: Soil fungi attacking ornamental plants – PP 1/40(2). In: EPPO Standards - Guidelines for the efficacy evaluation of plant protection products, 2 (pp 62-66). Paris, France: OEPP/EPPO.

Finney, D.J. (1971). Probit analysis: a statistical treatment of the sigmoid response curve (3rd ed.). Cambridge, UK: Cambridge university press.

Gaskill, J.O. (1968). Breeding for Rhizoctonia resistance in sugarbeet. Journal of the American Society of Sugar Beet Technologists, 15, 107-119.

Gerik, J.S., & Hanson, B.D. (2011). Drip application of methyl bromide alternative chemicals for control of soilborne pathogens and weeds. Pest Management Science, 67(9), 1129-1133.

Isman, M.B. (2000). Plant essential oils for pest and disease management. Crop Protection, 19(8-10), 603-608.

Ivanović, M., & Ivanović, M. (2007). Ima li alternative metil bromidu? (Are there alternatives for methyl bromide?). Biljni lekar, 35(6), 609-615.

Kalemba, D., & Kunicka, A. (2003). Antibacterial and antifungal properties of essential oils. Current Medicinal Chemistry, 10(10), 813-829.

Leroux, P., & Gredt, M. (1972). Etude de l’action in–vitro des fongicides, methode de l’incorporation ou milieu. Laboratorie de Phytopharmacie–INRA, Versailles, 1-10.

Löcher, F.J., & Lorenz, G. (1991). 4. Methods for monitoring the sensitivity of Botrytis cinerea to dicarboximide fungicides. EPPO Bulletin, 21(2), 341-354.

Markham, J.L. (1999). Biological activity of tea tree oil (pp 169-190). In: I. Southwell & R. Lowe, Tea tree, the genus Melaleuca. Amsterdam, Netherlands: Harwood Academic Publishers.

Mijatović, M., Obradović, A., & Ivanović M. (2007). Zaštita povrća od bolesti, štetočina i korova. Smederevska Palanka, Serbia: AgroMivas.

Narayanasamy, P. (2001). Plant pathogen detection and disease diagnosis (2nd ed.). Boca Raton, FL, USA: CRC Press.

Ogoshi, A. (1987). Ecology and pathogenicity of anastomosis and intraspecific groups of Rhizoctonia solani Kuhn. Annual Review of Phytopathology, 25, 125-143.

Pandey, A., & Palni, L.M.S. (1997). Bacillus species: the dominant bacteria of the rhizosphere of established tea bushes. Microbiological Research, 152(4), 359-365.

Pritchard, S.G. (2011). Soil organisms and global climate change. Plant Pathology, 60(1), 82-99.

Rini, C.R., & Sulochana, K.K. (2006). Management of seedling rot of chilli (Capsicum annuum L.) using Trichoderma spp. and fl uorescent pseudomonads (Pseudomonas fl uorescens). Journal of Tropical Agriculture, 44(1-2), 79-82.

Ryder, M.H., Yan, Z., Terrace, T.E., Rovira, A.D., Tang, W., & Correll, R.L. (1998). Use of strains of Bacillus isolated in China to suppress take-all and rhizoctonia root rot, and promote seedling growth of glasshouse-grown wheat in Australian soils. Soil Biology and Biochemistry, 31(1), 19-29.

Sharma, M., Gupta, S.K., & Sharma, T.R. (2005). Characterization of variability in Rhizoctonia solani by using morphological and molecular markers. Journal of Phytopathology, 153(7-8), 449-456.

Swiader, M., Pronczuk, M., & Niemirowicz-Szczyt, K. (2002): Resistance of Polish lines and hybrids of watermelon [Citrullus lanatus (Th unb.) Matsum et Nakai] to Fusarium oxysporum at the seedling stage. Journal of Applied Genetics, 43, 161-170.

UNEP (1994). 1994 Report of the Methyl Bromide Technical Options Committee for the 1995, Assessment of the UNEP Montreal protocol on substances that deplete the ozone layer. Nairobi, Kenya: United Nations Environment Programme, Methyl Bromide Technical Options Committee.

Whipps, J. M., & Lumsden, R. D. (2001). Commercial use of fungi as plant disease biological control agents: status and prospects. In: Butt, T.M., Jackson, C., & Magan, N. (eds.), Fungal biocontrol agents: progress, problems and potential, 9-22.

White, T.J., Bruns, T., Lee, S., & Taylor, J. (1990). Amplifi cation and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR protocols: a guide to methods and applications, 18(1), 315-322.

Wood, P.M., & Hollomon, D.W. (2003). A critical evaluation of the role of alternative oxidase in the performance of strobilurin and related fungicides acting at the Qo site of complex III. Pest Management Science, 59(5), 499-511.

Ziogas, B.N., Baldwin, B.C., & Young, J.E. (1997). Alternative respiration: a biochemical mechanism of resistance to azoxystrobin (ICIA 5504) in Septoria tritici. Pesticide Science, 50(1), 28-34.

Published
2020/12/17
Section
Original Scientific Paper