Possibility of environmentally-safe casing soil disinfection for control of cobweb disease of button mushroom

  • Ivana S Potočnik Institute of Pesticides and Environmental Protection, Laboratory for Applied Phytopathology, Banatska 31b, 11080 Belgrade
  • Emil Rekanović Institute of Pesticides and Environmental Protection, Laboratory for Applied Phytopathology, Banatska 31b, 11080 Belgrade
  • Miloš Stepanović Institute of Pesticides and Environmental Protection, Laboratory for Applied Phytopathology, Banatska 31b, 11080 Belgrade
  • Svetlana Milijašević-Marčić Institute of Pesticides and Environmental Protection, Laboratory for Applied Phytopathology, Banatska 31b, 11080 Belgrade
  • Biljana Todorović Institute of Pesticides and Environmental Protection, Laboratory for Applied Phytopathology, Banatska 31b, 11080 Belgrade
  • Ljiljana Nikolić-Bujanović IHIS Techno-Experts, Batajnički put 23, 11080, Belgrade
  • Milan Čekerevac IHIS Techno-Experts, Batajnički put 23, 11080, Belgrade
Keywords: Soil disinfection, Colloidal silver, Peracetic acid, Prochloraz, Cladobotryum dendroides, Agaricus bisporus,

Abstract


The soil-borne pathogen Cladobotryum dendroides causes cobweb disease of button mushroom (Agaricus bisporus) and its significant yield losses. Casing soil disinfection by toxic formaldehyde is a widespread practice. The aim of this study was to investigate the potential of two environmentally friendly substances, colloidal silver and peracetic acid, against C. dendroides. Their biological efficacy (impact on mushroom yield), effectiveness (disease control) and type of interactions between them and the fungicide prochloraz-manganese were evaluated.

Black peat/lime casing soil was applied to a colonized substrate with the white button mushroom strain 737, then inoculated with C. dendroides and treated with the fungicide prochloraz-manganse and two environmentally friendly disinfectants based on peracetic acid and colloidal silver. The effects of fungicides on mushroom productivity were evaluated as biological efficacy and calculated as a ratio of fresh weight of total mushroom yield to the weight of dry substrate. Fungicide effectiveness and synergy factor were calculated by Abbott’s (1925) formula. Tests for synergism between prochloraz-manganese and both other substances were performed using Limpel’s formula.

The highest biolgical efficacy, exceeding 92.00, was achieved in treatments with prochloraz-manganese, applied alone or in combination with both other disinfectants. The highest effectiveness of 93.33% was attained in treatments with peracetic acid combined with prochloraz-manganese. Trials against cobweb disease revealed a synergistic reaction between the fungicide and peracetic acid and antagonistic between the fungicide and colloidal silver.

Peracetic acid provided better disease control, compared to colloidal silver applied alone or in combination with the fungicide. Based on these findings, peracetic acid should be recomended as an environmentally friendly casing soil disinfectant against cobweb disease of A. bisporus.

References

Abbott, W.S. (1925). A method of computing the effectiveness of the insecticide. Journal of Economic Entomology, 18, 265-267.

Abosriwil, S.O., & Clancy, K.J. (2002). A protocol for evaluation of the role of disinfectants in limiting pathogens and weed moulds in commercial mushroom production. Pest Management Science, 58, 282-289.

Ašanin, M., & Mišić, D. (2006). Ispitivanje efikasnosti Perala-S u uslovima in vitro. Veterinarski glasnik, 60, 61-69.

Chikthimmah, N. (2006). Microbial ecology of mushroom casing soils and preharvest strategies to enhance safety and quality of fresh mushroom. (PhD thesis). Pennsylvania State University, Department of Food Science, USA.

Chrysayi-Tokousbalides, M., Kastanias, M.A., Philippoussis, A., & Diamantopoulou, P. (2007). Selective fungitoxicity of famaxadone, tebuconazole and trifloxystrobin between Verticillium fungicola and Agaricus bisporus. Crop Protection, 26, 469-475.

Čekerevac, M.I., Simičić, M.V., Rakin, P.M., Popović, N.H., Nikolić-Bujanović, Lj.N., & Rakin, D.P. (2006). Ekološko dezinfekciono sredstvo: dobijanje, dezinfekciono dejstvo i efikasnost. Hemijska industrija, 60, 180-187.

Durisić, S., Milosević, V., Visacki, M., & Luka, G. (1990). Study of viral sensitivity to the disinfectant Peral-S using the floating technique. Medicinski pregled, 43, 293-294.

Fletcher, J.T., & Ganney, G.W. (1968). Experiments on the biology and control of Mycogone perniciosa Mang. Mushroom Science, 7, 221-237.

Gea, F.J., Tello, J., & Navarro, M. (2010). Efficacy and effect on yield of different fungicides for control of wet bubble disease of mushroom caused by the mycoparasite Mycogone perniciosa. Crop Protection, 29, 1021-1025.

Gershenfeld, L., & Davis, D.E. (1952). Effect of peracetic acid on some thermoaciduric bacteria. American Journal of Pharmacy, 124, 337-342.

Greenspan, F.P., & MacKellar, D.G. (1951). The application of peracetic acid germicidal washes to mold control of tomatoes. Food Technology, 5, 95-97.

Lewandowski, D.J., & Hayes, A.J. (2009). Surprising results for a search for effective disinfectants for Tobacco Mosaic Virus – Contaminated tools. Plant Disease, 94, 542-550.

Milijašević-Marčić, S., Todorović, B., Potočnik, I., Stepanović, M., & Rekanović, E. (2012). First report of Pseudomonas tolaasii on Agaricus bisporus in Serbia. Phytoparasitica, 40, 299-303.

Morones, J.R., Elechiguerra, J.L., Camacho, A., Holt, K., Kouri, J.B., Ramírez, J.T., & Yacaman, M.J. (2005). The bactericidal effect of silver nanoparticles. Nanotechnology, 16, 2346-2353.

Nikolić-Bujanović, Lj., Popović, N., Simičić, M., Čekerevac, M., & Rakin, P. (2006). Ecocute ecological disinfectant. Jugoslovenska medicinska biohemija, 25, 263-267.

Potočnik, I., Vukojević, J., Stajić, M., Rekanović, E., Stepanović, M., Milijašević, S., & Todorović, B. (2010). Toxicity of biofungicide Timorex 66 EC to Cladobotryum dendroides and Agaricus bisporus. Crop Protection, 29, 290-294.

Potočnik, I., Vukojević, J., Stajić, M., Tanović, B., & Todorović, B. (2008): Fungicide sensitivity of selected Verticillium fungicola isolates from Agaricus bisporus farms. Archives of Biological Sciences, 60, 151-158.

Richer, D.L. (1987). Synergism - a patent view. Pesticide Science, 19, 309-315.

Rutala, A.W. & Weber, D.J. (2008). Guideline for disinfection and sterilization in healthcare facilities, healthcare infection control practices advisory committee (HICPAC). Retrieved from US Department of Health and Human Services, Center for Disease Control and Prevention, CDC http://www.cdc.gov/hicpac/Disinfection_Sterilization/8_0Iodophors.html [accessed 08 03 2014]

Savić, M., Andjelković I., Duvnjak D., Matijašević D., Avramović A., Pešić-Mikulec D., & Nikšić M. (2012). The fungistatic activity of organic selenium and its application to the production of cultivated mushrooms Agaricus bisporus and Pleurotus spp. Archives of Biological Sciences, 64, 1455-1463.

Sharma, M., & Guleria, D.S. (1999). Effect of formaldehyde as casing mixture treatment at different temperatures on the mycoflora and productivity of Agaricus bitorquis (Quel.) Sacc. Journal of Hill Research, 12, 61-62.

Tanović B., Potočnik I., Delibašić G., Ristić M., Kostić M., & Marković M. (2009). In vitro effect of essential oils from aromatic and medicinal plants on mushroom pathogens: Verticillium fungicola var. fungicola, Mycogone perniciosa, and Cladobotryum sp. Archives of Biology Sciences, 61, 231-238.

Todorović, B., Milijašević-Marčić, S., Potočnik, I., Stepanović, M., Rekanović, E., Nikolić-Bujanović, Lj., & Čekerevac, M. (2012). In vitro activity of antimicrobial agents against Pseudomonas tolaasii, pathogen of cultivated button mushroom. Journal of Environmental Sciences and Health, Part B, 47, 175-179.

Varner, K., Sanford, J., El-Badawy, A., Feldhake, D. &Venkatapathy, R. (2010). State of the science literature review: Everything about nanosilver and more. In A. El-Badawy, D. Feldhake & R. Venkatapathy (Eds.), Scientific, technical, research, engineering and modeling support final review (pp. 1-363). Washington, DC: US Environmental Protection Agency (EPA).

Wong, W.C., & Preece T.F. (1985). Pseudomonas tolaasii in mushroom (Agaricus bisporus) crops: effects of sodium hypochlorite on the bacterium and on blotch disease severity. Journal of Applied Bacteriology, 58, 259-267.

Published
2015/02/10
Section
Original Scientific Paper