Monitoring of Erwinia amylovora in Montenegro
Abstract
Recent studies of Erwinia amylovora in Montenegro, conducted from 2012 to 2014, indicated that the bacterium was widespread in the northern, continental part of the country, where the most important fruit-growing regions are situated. The presence of the bacterium was confirmed on quince, pear, apple, medlar and hawthorn. Pathogenic, cultural and biochemical characteristics of E. amylovora strains sampled from pome fruit species and indigenous flora in Montenegro had been studied previously.
In the present study, serological tests were used for identification of E. amylovora strains originating from pome fruit trees and indigenous plants. Monitoring of E. amylovora and collection of samples with symptoms of bacterial fire blight from different hosts and locations were performed in Montenegro from 2012 to 2014. Isolation of the bacterium on nutrient medium produced a large number of isolates, whose pathogenicity was confirmed on immature pear fruits. Twenty-seven strains of the bacterium, originating from three pome fruit species (quince, pear and apple) and one indigenous species (hawthorn) were selected for serological analyses. Two applied serological methods, ELISA and IF test, enabled rapid detection of the bacterium and simultaneous examination of a large number of samples over a short period of time. Serological analyses showed high homogeneity in antigenic structure of the studied E. amylovora strains sampled from quince, pear, apple and hawthorn from nine locations in Montenegro.
References
Arsenijević, M. (1997). Bakterioze biljaka (3. izd). Novi Sad: S Print, Univerzitet u Novom Sadu, Poljoprivredni fakultet.
Arsenijević, M., Đurišić, S., & Mitrev, S. (1994). Serološko identifikovanje izolata bakterije Erwinia amylovora parazita jabučastih voćaka. Zaštita bilja, 45(4), 273-278.
Arsenijević, M., & Gavrilović, V. (2007). Praktični priručnik o bakterioznoj plamenjači voćaka i ukrasnih biljaka. Beograd: Institut za zaštitu bilja i životnu sredinu.
Balaž, J., Radunović, D., & Krstić, M. (2012). Status of Erwinia amylovora in Montenegro. In Proceedings of International Symposium: Current Trends in Plant Protection (pp 373-378). Belgrade, Serbia: Institute for Plant Protection and Environment.
Gavrilović, V. (2009). Erwinia amylovora - parazit jabučastih voćaka u Srbiji. In Zbornik radova, Inovacije u voćarstvu, II savetovanje (pp 107-117). Beograd: Poljoprivredni fakultet.
Gavrilović, V., Milijašević, S., Todorović, B., Živković, S., & Trkulja, N. (2008). Erwinia amylovora – prouzrokovač nekroze korenovog vrata stabla jabuke. Pesticidi i fitomedicina, 23(1), 17-23.
Janse, J.D. (2006). Phytobacteriology - Principles and practice. Boston, MA: CABI Publishing.
Lelliott, R.A., & Stead, D.E. (1987). Methods for the diagnosis of bacterial disease of plants. In British Society for Plant Pathology (p 200). Oxford: Blackwell Scientific Publications.
Mclaughlin, R.J., Chen, T.A., & Wells, J.M. (1989). Monoclonal antibodies against Erwinia amylovora: Characterization and evaluation of a mixture for detection by enzyme-linked immunosorbent assay. Phytopathology, 79(5), 610-613.
Obradović, A., Vučinić, Z., & Gavrilović, V. (2003). Epifitotična pojava bakteriozne plamenjače dunje u Srbiji i Crnoj Gori. In Zbornik rezimea VI savetovanje o zaštiti bilja (Zlatibor) (p 84). Beograd: Društvo za zaštitu bilja Srbije.
OEPP/EPPO (2013). Diagnosis of Erwinia amylovora. Protocol for the diagnosis of quarantine organism. SMT Project SMT-4-CT98-2252. Bulletin OEPP/EPPO Bulletin, 43, 21-45.
Panić, M., & Arsenijević, M. (1996). Bakteriozna plamenjača voćaka i ukrasnih biljaka - Erwinia amylovora. Beograd: Zajednica za voće i povrće. Novi Sad: Poljoprivredni fakultet.
Paulin, J.P. (2000). Erwinia amylovora: General characteristics, biochemistry and serology. In J.L. Vanneste (Ed.), Fire blight: The disease and its causative agent, Erwinia amylovora. (pp 87-117). Hamilton, New Zealand: CABI Publishing.
Psallidas, P.G., & Tsiantos, J. (2000). Chemical control of fire blight. In J.L. Vanneste (Ed.), Fire blight: The disease and its causative agent, Erwinia amylovora (pp 199-234). Hamilton, New Zealand: CABI Publishing.
Radunović, D., & Gavrilović, V. (2013). Monitoring Erwinia amylovora u Crnoj Gori. In Zbornik rezimea radova XII Savetovanje o zaštiti bilja (Zlatibor) (pp 172-173). Beograd: Društvo za zaštitu bilja Srbije.
Radunović, D., Gavrilović, V., & Krstić, M. (2013). Bacterial characteristics of Erwinia amylovora strains originating from pome fruits and ornamental plants in Montenegro. Zaštita bilja/Plant Protection (Belgrade), 64(1), 7-13.
Schaad, N.W., Jones, J.B., & Chun, W. (2001). Laboratory guide for identification of plant pathogenic bacteria,(3rd ed.). St. Paul, MN: APS Press.
Statistički godišnjak Crne Gore. (2013). Podgorica: Zavod za statistiku Crne Gore.
Steiner, P.W. (2000). Integrated orchard and nursery management for the control of fire blight. In J. Vanneste (Ed.), Fire blight: The disease and its causative agent, Erwinia amylovora (pp 339-359). Hamilton, New Zealand: CABI Publishing.
Turechek, W.W. (2004). Apple diseases and their management. In S.A.M.H. Naqvi (ed.), Diseases of fruits and vegetables, diagnosis and management, Vol. 1.(pp 1-108) The Netherlands: Kluwer Academic Publishers.
van der Zwet, T., & Beer, S.V. (1999). Fire blight: Its nature, prevention and control. A practical guide to integrated diseases management. Agricultural Bulletin, 631, 97.
van der Zwet, T., & Keil, H.L. (1979). Fire blight: A bacterial disease of Rosaceous plants. In Fire blight – A bacterial disease of Rosaceous plants (p 200). Washington, DC: U.S. Department of Agriculture.
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