Antifungal activity of plant essential oils and selected Pseudomonas strains against Phomopsis theicola

  • Mira Starović Institute for Plant Protection and Environment, Teodora Drajzera 9, 11040 Belgrade
  • Danijela Ristić Institute for Plant Protection and Environment, Teodora Drajzera 9, 11040 Belgrade
  • Goran Aleksić Institute for Plant Protection and Environment, Teodora Drajzera 9, 11040 Belgrade
  • Snežana Pavlović Institute for Medicinal Plant Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade
  • Mehmet Musa Özcan Department of Food Engineering, Faculty of Agriculture, Selçuk University, 42079 Konya
  • Magdalena Knežević Institute of Soil Science, Teodora Drajzera 7, 11040 Belgrade
  • Dragana Jošić Institute of Soil Science, Teodora Drajzera 7, 11040 Belgrade
DOI: https://doi.org/10.2298/pif.v32i2.13595
Keywords: Essential oils, Pseudomonas spp, PGPR, Phomopsis theicola, Antifungal activity,

Abstract


Development of natural plant protection products as an alternative to synthetic fungicides is of significant importance regarding the environment. This study was carried out with an objective to investigate in vitro antifungal activities of several essential oils extracted from oregano, basil, myrtle and Turkish pickling herb, and the plant growth-promoting rhizobacteria in the genus Pseudomonas, against the phytopathogenic fungus Phomopsis theicola. Microdilution methods were used to determine the minimum inhibitory concentrations (MIC) of selected antimicrobial essential oils (EOs). All EOs exhibited significant levels of antifungal activity against the tested fungal isolates. The oregano EO was found the most potent one (MIC – 5.5 μg/mL), followed by basil (MIC – 75.0μg/mL), myrtle (MIC – 775 μg/mL) and Turkish pickling herb (MIC - 7750 μg/mL). Inhibition of Ph. theicola mycelial growth was observed for all tested Pseudomonas spp. strains. K113 and L1 strains were highly effective and achieved more than 60% of fungal growth inhibition using the overnight culture and more than 57% inhibition by applying cell-free supernatants of both strains. A future field trial with K113 and L1 cultures and cell-free supernatants, containing extracellular metabolites toward Ph. theicola, will estimate their effectiveness and applicability as an alternative to chemical protection of apple trees.

References

Carmo, E.S., Lima, E.O., & Souza, E.L. (2008). The potential of Origano vulgare L. essential oil in inhibiting the growth of the growth of some food-related Aspergillus species. Brazilian Journal of Microbiology, 39(2), 362-367.

Chaurasia, B., Pandey, A., Palni, L.M.S., Trivedi, P., Kumar, B., & Colvin, N. (2005). Diffusible and volatile compounds produced by an antagonistic Bacillus subtilis strain cause structural deformations in pathogenic fungi in vitro. Microbiological Research, 160(1), 75–81.

Cloete, M., Fourie, P.H., Damm, U., Crous, P.W., & Mostert, L. (2011). Fungi associated with die-back symptoms of apple and pear trees, a possible inoculum source of grapevine trunk disease pathogens. Phytopathologia Mediterranea, 50(S), 176-190.

Daouk, R.K., Dagher, S.M., & Sattout, E.J. (1995). Antifungal activity of the essential oil of Origanum syriacum L. Journal of Food Protection, 58(10), 1147-1149.

Girish, K., Bhat, S., & Raveesha, K. (2009). In vitro evaluation of antagonistic microorganisms for the control of die back of neem causal agent Phomopsis azadirachtae. Journal of Plant Protection Research, 49(4), 362-368. doi https://doi.org/10.2478/v10045-009-0056-7

Girish K., Shankara, Bhat, S., K. A., & Raveesha, K.A. (2012). Synergistic effect of combinations of fungicides and bacterial extracts against Phomopsis azadirachtae causing die-back of neem. African Journal of Microbiology Research, 6(2), 385-392. doi 10.5897/AJMR11.1154

Haggag, W.M., Saleh, M.A.E., Mostafa, I., & Adel, N. (2013). Mass production, fermentation, formulation of Pseudomonas putida for controlling of die back and phomopsis diseases on grapevine. Advances in Bioscience & Biotechnology, 4(6), 741-750.

Jošić, D., Ćirić, A., Soković, M., Stanojković-Sebić, A., Pivić, R., Lepšanović, Z., & Glamočlija, J. (2015). Antifungal activities of indigenous plant growth promoting Pseudomonas spp. from alfalfa and clover rhizosphere. Frontiers in Life Science, 8(2), 131-138.

Jošić, D., Protolipac, K., Starović, M., Stojanović, S., Pavlović, S., Miladinović, M., & Radović, S. (2012). Phenazines producing Pseudomonas isolates decrease Alternaria tenuissima growth, pathogenicity and disease incidence on cardoon. Archives of Biological Sciences, 64(4), 1495-1503.

Kamilova, F., Validov, S., Azarova, T., Mulders, I., & Lugtenberg, B. (2005). Enrichment for enhanced competitive plant root tip colonizers selects for a new class of biocontrol bacteria. Environmental Microbiology, 7(11), 1809-1817.

Lopez-Reyes, J.G., Spadaro, D., Prelle, A., Garibaldi, A., & Gullino, M.L. (2013). Efficacy of plant essential oils on postharvest control of rots by fungi on different stone fruits in vivo. Journal of Food Protection, 76(4), 631-639.

Maksimov, I.V., Abizgil’dina, R.R., & Pusenkova, L.I. (2011). Plant growth promoting rhizobacteria as alternative to chemical crop protectors from pathogens. Applied Biochemistry & Microbiology, 47(4), 333-345.

Neeraja, C., Anil, K., Purushotham, P., Suma, K., Sarma, P., & Moerschbacher, B.M. (2010). Biotechnological approaches to develop bacterial chitinases as a bioshield against fungal diseases of plants. Critical Reviews in Biotechnology, 30(3), 231–241.

Patkowska, E., & Błażewicz-Woźniak, M. (2013). May the post-culture liquids of bacteria influence on soybean [Glycine max (L.) Merrill] healthiness? Acta Scientiarum Polonorum Hortorum Cultus 12(3), 171-182.

Pivić, R., Starović, M., Delić, D., Rasulić, N., Kuzmanović, Đ., Poštić, D., & Jošić D. (2015). Bacterial antagonists Bacillus sp. Q3 and Pseudomonas chlororaphis Q16 capable to control wheat powdery mildew in wheat. Romanian Biotechnological Letters, 20(3), 10448-10460.

Podile, A.R., & Kishore, G.K. (2006). Plant growth-promoting rhizobacteria. In Gnanamanickam S.S. (Ed), Plantassociated bacteria (pp 195–230). Springer; Netherlands.

Srinivas, C., Niranjana, S.R., & Shetty, H.S. (2005). Effect of bioagents and fungicides against Phomoposis vexans and on seed quality of brinjal. Crop Improvement, 32(1), 95-101.

Starović, M., Ristić, D., Pavlović, S., Ristić, M., Stevanović, M., Aljuhaimi,F. ... Ozcan, M.M. (2016). Antifungal activities of different essential oils against anise seeds mycopopulations. Journal of Food Safety and Food Quality, 67, 72-78.

Stević, T., Berić, T., Šavikin, K., Soković, M., Godjevac, D., Dimkić, I., & Stanković, S. (2014). Antifungal activity of selected essential oils against fungi isolated from medicinal plant. Industrial Crops and Products, 55, 116-122.

Sutton T.B., Aldwinckle H.S., Angello A.M., & Walgenbach J.F. (eds.) (2014). Compendium of apple and pear diseases and pests (2nd ed). St. Paul, MN, USA: American Phytopathological Society.

Tavassoli, S., Mousavi, S.M., Emam-Djomeh, Z., & Razavi SH. (2011). Comparative study of the antimicrobial activity of Rosmarinus officinalis L. essential oil and methanolic extract. Middle-East Journal of Scientific Research, 9(4), 467-471.

Udayanga, D., Liu, X., McKenzie, E.H.C., Chukeatirote, E., Bahkali, A.H.A., & Hyde, K.D. (2011). The genus Phomopsis: biology, applications, species concepts and names of common phytopathogens. Fungal Diversity, 50, 189-225.

Van Niekerk, J.M., Groenewald, J.Z., Verkley, G.J.M., Fourie, P.H., Wingfield, M.J., & Crous, P.W. (2004). Systematic reappraisal of Coniella and Pilidiella, with specific reference to species occurring on Eucalyptus and Vitis in South Africa. Mycological Research, 108(3), 283-303.

Wolf, A., Fritze, A., Hagemann, M., & Berg G. (2002). Stenotrophomonas rhizophila sp. nov., a novel plant associated bacterium with antifungal properties. International Journal of Systematic and Evolutionary Microbiology, 52(6), 1937–1944.

Zalewska E., Machowicz-Stefaniak Z., & Krol E. (2004). The biotic effect of phyllosphaere microorganisms on some fungi pathogenic to plants. Agronomijas Vēstis/Latvian Journal of Agronomy, 7, 155-157.

Published
2017/10/02
Issue
Vol. 32 No. 2 (2017): Pesticidi i fitomedicina
Section
Original Scientific Paper

Authors retain copyright of the published papers and grant to the publisher the non-exclusive right to publish the article, to be cited as its original publisher in case of reuse, and to distribute it in all forms and media.

The published articles will be distributed under the Creative Commons Attribution ShareAlike 4.0 International license (CC BY-SA). It is allowed to copy and redistribute the material in any medium or format, and remix, transform, and build upon it for any purpose, even commercially, as long as appropriate credit is given to the original author(s), a link to the license is provided, it is indicated if changes were made and the new work is distributed under the same license as the original.

Users are required to provide full bibliographic description of the original publication (authors, article title, journal title, volume, issue, pages), as well as its DOI code. In electronic publishing, users are also required to link the content with both the original article published in Pesticidi i fitomedicina (Pesticides and Phytomedicine) and the licence used.

Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.