Antifungal activity of plant essential oils and selected Pseudomonas strains against Phomopsis theicola

  • Mira Starović Institute for Plant Protection and Environment, Teodora Drajzera 9, 11040 Belgrade
  • Danijela Ristić Institute for Plant Protection and Environment, Teodora Drajzera 9, 11040 Belgrade
  • Goran Aleksić Institute for Plant Protection and Environment, Teodora Drajzera 9, 11040 Belgrade
  • Snežana Pavlović Institute for Medicinal Plant Research “Dr Josif Pančić”, Tadeuša Košćuška 1, 11000 Belgrade
  • Mehmet Musa Özcan Department of Food Engineering, Faculty of Agriculture, Selçuk University, 42079 Konya
  • Magdalena Knežević Institute of Soil Science, Teodora Drajzera 7, 11040 Belgrade
  • Dragana Jošić Institute of Soil Science, Teodora Drajzera 7, 11040 Belgrade
DOI: https://doi.org/10.2298/pif.v32i2.13595
Keywords: Essential oils, Pseudomonas spp, PGPR, Phomopsis theicola, Antifungal activity,

Abstract


Development of natural plant protection products as an alternative to synthetic fungicides is of significant importance regarding the environment. This study was carried out with an objective to investigate in vitro antifungal activities of several essential oils extracted from oregano, basil, myrtle and Turkish pickling herb, and the plant growth-promoting rhizobacteria in the genus Pseudomonas, against the phytopathogenic fungus Phomopsis theicola. Microdilution methods were used to determine the minimum inhibitory concentrations (MIC) of selected antimicrobial essential oils (EOs). All EOs exhibited significant levels of antifungal activity against the tested fungal isolates. The oregano EO was found the most potent one (MIC – 5.5 μg/mL), followed by basil (MIC – 75.0μg/mL), myrtle (MIC – 775 μg/mL) and Turkish pickling herb (MIC - 7750 μg/mL). Inhibition of Ph. theicola mycelial growth was observed for all tested Pseudomonas spp. strains. K113 and L1 strains were highly effective and achieved more than 60% of fungal growth inhibition using the overnight culture and more than 57% inhibition by applying cell-free supernatants of both strains. A future field trial with K113 and L1 cultures and cell-free supernatants, containing extracellular metabolites toward Ph. theicola, will estimate their effectiveness and applicability as an alternative to chemical protection of apple trees.

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Published
2017/10/02
How to Cite
Starović, M., Ristić, D., Aleksić, G., Pavlović, S., Özcan, M. M., Knežević, M., & Jošić, D. (2017). Antifungal activity of plant essential oils and selected Pseudomonas strains against Phomopsis theicola. Pesticides and Phytomedicine / Pesticidi I Fitomedicina, 32(2). https://doi.org/10.2298/pif.v32i2.13595
Issue
Vol. 32 No. 2 (2017): Pesticidi i fitomedicina
Section
Original Scientific Paper

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