Incidence and distribution of Leek yellow stripe virus in Allium crops in Serbia
Abstract
Leek yellow stripe virus (LYSV) is one of the most frequent and important viruses in leek and garlic crops worldwide. In Serbia this virus is found both in leek and garlic, and often at high percentages. During two consecutive years, 2012 and 2013, a total 92 samples were collected from 11 inspected leek-, garlic- and onion-growing locations and they were analyzed for the presence of LYSV using DAS-ELISA. LYSV was detected in 31.5% of the tested samples. In 2012, the presence of LYSV was only detected in leek plants, and in 55.6% of the tested samples. During 2013, LYSV was detected in 85% of leek and 58.3% of garlic samples. In total, LYSV was detected in 56.4% of leek samples and 17.1% garlic samples. LYSV incidence was confirmed using RT-PCR with LYSV specific primers amplifying 1020 bp fragment representing coat protein and part of nuclear inclusion B genes. Molecular identification was confirmed by sequencing of two selected isolates, 181-13 (MG242625) from garlic and 298-13 (MG242624) from leek, and comparing them to the GenBank sequences of LYSV. Phylogenetic analysis of 55 sequences of LYSV from all over the world showed some correlation between host plant and geographical origin of the isolates, forming five separate clades. Two Serbian LYSV isolates fell into distant clades. The Serbian leek isolate 298-13 of LYSV belongs to clade B, while isolate 181-13 originating from garlic belongs in clade E.
References
Abd El-Wahab A.S. (2009). Aphid-transmission efficiency of two main viruses on garlic in Egypt, Onion yellow dwarf virus (OYDV-G) and Leek yellow stripe virus (LYSV-G). Academic Journal of Entomology, 2(1), 40-42.
Aleman-Verdaguer, M.E., Goudou-Urbino, C., Dubern, J., Beachy, R.N., & Fauquet, C. (1997). Analysis of the sequence diversity of the P1, HC, P3, NIb and CP genomic regions of several yam mosaic potyvirus isolates: Implications for the intraspecies molecular diversity of potyviruses. Journal of General Virology,78(6), 1253-1264.
Bagi, F., Gvozdanović-Varga, J., Budakov, D., Stojšin, V., Janićijević, M., Šantić, M., & Jasnić, S. (2010). Zaraženost belog luka virusom žute patuljavosti luka (OYDV) i virusom žute prugavosti praziluka (LYMV) (pp 57-58). In X Savetovanje o zaštiti bilja, Zlatibor.
Bateson, M.F., Henderson, J., Chaleeprom, W., Gibbs, A.J., & Dale, J.L. (1994). Papaya ringspot potyvirus: Isolate variability and the origin of PRSV type P (Australia). Journal of General Virology, 75(12), 3547-3553. doi:10.1099/0022-1317-75-12-3547
Block, E. (2010). Garlic and other Alliums: The lore and the science. Cambridge, UK: Royal society of Chemistry.
Bos, L. (1981). Leek yellow stripe virus. CMI/AAB Descriptions of plant viruses, no. 240.
Bos, L., Huijberts, N., Huttinga, H., & Maat, D.Z. (1978). Leek yellow stripe virus and its relationships to onion yellow dwarf virus; characterization, ecology and possible control. Netherlands Journal of Plant Pathology, 84(5), 185-204. doi:10.1007/BF02650386
Bremer, H. (1937). Über die bisher fälschlich ‘Zwiebelrotz’ genannte Gelbstreifigkeit an Zwiebelsamenträgern. Phytopathologische Zeitschrift, 10, 79-105.
Brewster, J.L. (1994). Onion and other vegetable Alliums. Wallingford, Oxforshire: CABI.
Chen, J., Chen, J.P., & Adams, M.J. (2002). Characterisation of some carla- and potyviruses from bulb crops in China. (Brief report). Archives of Virology, 147(2), 419-428. pmid:11890533. doi:10.1007/s705-002-8330-y
Chodorska, M., Paduch-Cichal, E., Kalinowska, E., Gaczkowska, O., Lis, M., Sierant, B., & Szyndel, M.S. (2014). First report of Leek yellow stripe virus in foreign and polish garlic plants in central Poland. Journal of Plant Pathology, 96(4 supp.), 4-120.
Clark, M.F., & Adams, A.N. (1977). Characteristics of the microplate method of enzyme-linked immunosorbent assay for the detection of plant viruses. Journal of General Virology, 34(3), 475-83. pmid:323416
Conci, V.C., Canavelli, A., Lunello, P., Di Rienzo, J., Nome, S.F., Zumelzu, G., & Italia, R. (2003). Yield losses associated with virus-infected garlic plants during five successive years. Plant Disease, 87(12), 1411-1415.
Conci, V.C., Lunello, P., Buraschi, D., Italia, R.R., & Nome, S.F. (2002). Variations of Leek yellow stripe virus concentration in garlic and its incidence in Argentina. Plant Disease, 86(10), 1085-1088.
Cornuet, P. (1959). Maladies a virus des plantes cultivees et methodes de lutte (p 440). Paris, France: Institut National de la Recherche Agronomique.
Delecolle, B., & Lot, H. (1981). Viroses de l’ail : I. - Mise en évidence et essais de caractérisation par immunoélectromicroscopie d’un complexe de trois virus chez différentes populations d’ail atteintes de mosaique. Agronomie, 1(9), 763-770. doi:10.1051/agro:19810908
Diekmann, M. (1997). FAO/IPGRI technical guidelines for the safe movement of germplasm. Allium spp. Rome, Italy: Food and Agriculture Organization of the United Nations, International Plant Genetic Resources Institute.
Dovas, C.I., Hatziloukas, E., Salomon, R., Barg, E., Shiboleth, Y., & Katis, N.I. (2001). Incidence of viruses infecting Allium spp. in Greece. European Journal of Plant Pathology, 107(7), 677-684. doi:10.1023/A:1011958914573
Dovas, C.I., & Vovlas, C. (2003). Viruses infecting Allium spp. in southern Italy. Journal of Plant Pathology, 85(2), 135.
Fajardo, T.V., Nishijima, M., Buso, J.A., Torres, A.C., Ávila, A.C., & Resende, R.O. (2001). Garlic viral complex: Identification of potyviruses and carlavirus in central Brazil. Fitopatologia Brasileira, 26(3), 619-626.
Fayad-André, M.D.S., Dusi, A.N., & Resende, R.O. (2011). Spread of viruses in garlic fields cultivated under different agricultural production systems in Brazil. Tropical Plant Pathology, 36(6), 341-349.
Fidan, H., & Baloglu, S. (2009). First report of Onion yellow dwarf virus and Leek yellow stripe virus in garlic in Turkey. Plant Disease, 93(6), 672.
Filho, P.A.M., Resende, R.O., Cordeiro, C.M.T., Buso, J.A., Torres, A.C., & Dusi, A.N. (2006). Viral reinfection affecting bulb production in garlic after seven years of cultivation under field conditions. European Journal of Plant Pathology, 116(2), 95-101.
Food and Agricultural Organization of the United Nations (FAO). (2011). Statistics. Retrieved from http://faostat.fao.org
Fritsch, R.M., Blattner, F.R., & Gurushidze, M. (2010). New classification of Allium L. subg. Melanocrommyum (Webb & Berthel.) Rouy (Alliaceae) based on molecular and morphological characters. Phyton (Horn), 49(2), 145-220.
Fritsch, R.M., & Friesen, N. (2002). Evolution, domestication and taxonomy. In Rabinowitch, H. D., Currah, L. (eds.), Allium crop science: Recent advances (pp. 5-30). Wallingford, UK: CABI. doi: 10.1079/9780851995106.0000
Fritsch, R.M., & Friesen, C.N. (2009). Allium oreotadzhikorum and Allium vallivanchense, two new species of Allium subg. Polyprason (Alliaceae) from the Central Asian republic Tajikistan. Feddes Repertorium, 120(3-4), 221-231.
García-Arenal, F., Fraile, A., & Malpica, J.M. (2001). Variability and genetic structure of plant virus populations. Annual Review of Phytopathology, 39, 157-186.
Grancini, P. (1951). Malattie da virus degli orteiggi il mosaic della cipolla. Flora, 6(19).
Gupta, N., Prabha, K., Islam, S., & Baranwal, V.K. (2013). First report of Leek yellow stripe virus in garlic from India. Journal of Plant Pathology, 95(4 supp.), S4.69-S4.77.
Ha, C., Revill, P., Harding, R.M., Vu, M., & Dale, J.L. (2008). Identification and sequence analysis of potyviruses infecting crops in Vietnam. Archives of Virology, 153(1), 45-60. pmid:17906829. doi:10.1007/s00705-007-1067-1
Katis, N.I., Maliogka, V.I., & Dovas, C.I. (2012). Viruses of the genus Allium in the Mediterranean region. Advances in Virus Research, 84, 163-208.
Klukáčková, J., Navrátil, M., & Duchoslav, M. (2007). Natural infection of garlic (Allium sativum L.) by viruses in the Czech Republic. Journal of Plant Diseases and Protection, 114(3), 97-100.
Korkmaz, S., & Cevik, B. (2009). Leek yellow stripe virus newly reported in Turkey. Plant Pathology, 58, 787.
Kupke, W. (1957). Die Gelbstreifigkeit, eine gefährliche Krankheit des Porrees Rheinische Monatsschrift für Gemüse Obst-Gartenbau, 45, 173.
Li, H., & Roossinck, M.J. (2004). Genetic bottlenecks reduce population variation in an experimental RNA virus population. Journal of Virology, 78(19), 10582-10587. pmid:15367625
Lot, H., Chovelon, V., Souche, S., & Delecolle, B. (1998). Effects of onion yellow dwarf and leek yellow stripe viruses on symptomatology and yield loss of three French garlic cultivars. Plant Disease, 82(12), 1381-1385.
Lunello, P., di Rienzo, J., & Conci, V.C. (2007). Yield loss in garlic caused by Leek yellow stripe virus Argentinean isolate. Plant Disease, 91(2), 153-158.
Lunello, P., Ducasse, D.A., Helguera, M., Nome, S.F., & Conci, V.C. (2002). An Argentinean isolate of Leek yellow stripe virus from leek can be transmitted to garlic. Journal of Plant Pathology, 84, 11-17.
Mavrič, I., & Ravnikar, M. (2005). A carlavirus serologically closely related to Carnation latent virus in Slovenian garlic. Acta Agriculturae Slovenica, 85(2), 343-349.
Milošević, D., Gvozdanović-Varga, J., Ignjatov, M., Nikolić, Z., Vučurović, I., Vučurović, A., & Stanković, I. (2015). First report of Onion yellow dwarf virus infecting shallot in Serbia. Plant Disease, 99(10), 1450-1450.
Mohammad, G., Kawas, H., & Al-Safadi, B. (2007). Survey of garlic viruses in southern Syria. Damascus University Journal for the Agricultural Sciences, 23(1), 255-265.
Oleas, A., & Arahana, V. (2016). First report of Leek yellow stripe virus, Shallot latent virus, and Onion yellow dwarf virus in garlic from Ecuador. Plant Disease, 100(1), 232.
Pappu, H.R., Hellier, B.C., & Dugan, F.M. (2005). First report of Onion yellow dwarf virus, Leek yellow stripe virus, and Garlic common latent virus in garlic in Washington State. Plant Disease, 89(2), 205.
Parrano, L., Afunian, M., Pagliaccia, D., Douhan, G., & Vidalakis, G. (2012). Characterization of viruses associated with garlic plants propagated from different reproductive tissues from Italy and other geographic regions. Phytopathologia Mediterranea, 51(3), 549-565.
Republički zavod za statistiku (RZS) Republike Srbije (2013). Statistički godišnjak biljne proizvodnje. Retrieved from http://webrzs.stat.gov.rs/WebSite/Default.aspx
Salomon, R., Koch, M., Levy, S., Gal-On, A., & Marshall, G. (1996). Detection and identification of the viruses forming mixed infection in garlic. In Symposium Proceedings No. 65: Diagnostics in Crop Production (pp 193-198). Farnham, UK; British Crop Protection Council.
Shiboleth, Y.M., Gal-On, A., Koch, M., Rabinowitch, H.D., & Salomon, R. (2001). Molecular characterisation of Onion yellow dwarf virus (OYDV) infecting garlic (Allium sativum L.) in Israel: Thermotherapy inhibits virus elimination by meristem tip culture. Annals of Applied Biology, 138(2), 187-195. doi:10.1111/j.1744-7348.2001.tb00101.x
Shukla, D.D., & Ward, C.W. (1988). Amino acid sequence homology of coat proteins as a basis for identification and classification of the Potyvirus group. Journal of General Virology, 69(11), 2703-2710. doi: 10.1099/0022-1317-69-11-2703
Stanković, I., Bulajić, A., Vučurović, A., Ristić, D., Milojević, K., Nikolić, D., & Krstić, B. (2012). First report of Tomato spotted wilt virus infecting onion and garlic in Serbia. Plant Disease, 96(6), 918.
Takaichi, M., Yamamoto, M., Nagakubo, T., & Oeda, K. (1998). Four garlic viruses identified by reverse transcriptionpolymerase chain reaction and their regional distribution in northern Japan. Plant Disease, 82(6), 694-698.
Takaki, F., Sano, T., Yamashita, K., Fujita, T., Ueda, K., & Kato, T. (2005). Complete nucleotide sequences of attenuated and severe isolates of Leek yellow stripe virus from garlic in northern Japan: Identification of three distinct virus types in garlic and leek world-wide. Archives of Virology, 150(6), 1135-1149. pmid:15703850. doi:10.1007/s00705-004-0482-9
Tamura, K., Peterson, D., Peterson, N., Stecher, G., Nei, M., & Kumar, S. (2011). MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Molecular Biology and Evolution, 28(10), 2731-2739. pmid:21546353
Testen, A.L., Mamiro, D.P., Meulia, T., Subedi, N., Islam, M., Baysal-Gurel, F., & Miller, S.A. (2014). First report of Leek yellow stripe virus in garlic in Ohio. Plant Disease, 98(4), 574.
Thompson, J.D., Higgins, D.G., & Gibson, T.J. (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22(22), 4673-4680. pmid:7984417. doi:10.1093/nar/22.22.4673
van Dijk, P. (1993). Survey and characterization of potyviruses and their strains of Allium species. Netherlands Journal of Plant Pathology, 99(2), 1-48.
Verhoyen, M., & Horvat, F. (1973). La striurechlorotique du porreau. 1. Identificatin de l’agent causal. Parasitica, 29, 16-28.
Vončina, D., Ćurić, K., Fabek, S., & Toth, N. (2016). First report of Onion yellow dwarf virus, Leek yellow stripe virus, and Garlic common latent virus on garlic in Croatia. Plant Disease, 100(3), 656.
Vučurović, A., Vučurović, I., Stanković, I., Bulajić, A., Nikolić, D., Teodorović, S., & Krstić, B. (2015). First report of Garlic common latent virus infecting garlic in Serbia. Plant Disease, 99(6), 894.
Vučurović, I., Vučurović, A., Nikolić, D., Bulajić, A., Milošević, D., Krstić, B., & Stanković, I. (2016). First report of Leek yellow stripe virus in leek in Serbia. Plant Disease, 100(1), 230.
Winiarczyk, K., Solarska, E., & Sienkiewicz, W. (2014). Prevalence of infections with Onion yellow dwarf virus, Leek yellow stripe virus and Garlic common latent virus in plants from the genus Allium. Acta Scientiarum Polonorum, Hortorum Cultus, 13(3), 123-133.
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