Antifungal activity of chitosan against Alternaria alternata and Colletotrichum gloeosporioides

Svetlana Živković; Miloš Stevanović; Sanja Đurović; Danijela Ristić; Stefan Stošić

doi:10.2298/

Svetlana Živković Institut za zaštitu bilja i životnu sredinu, T. Drajzera 9, 11040 Beograd
Miloš Stevanović Institut za zaštitu bilja i životnu sredinu, T. Drajzera 9, 11040 Beograd
Sanja Đurović Institut za zaštitu bilja i životnu sredinu, T. Drajzera 9, 11040 Beograd
Danijela Ristić Institut za zaštitu bilja i životnu sredinu, T. Drajzera 9, 11040 Beograd
Stefan Stošić Institut za zaštitu bilja i životnu sredinu, T. Drajzera 9, 11040 Beograd

DOI: https://doi.org/10.2298/

Ključne reči: Chitosan||, ||Hitosan, Antifungal activity||, ||Antifungalno delovanje, Postharvest pathogens||, ||Skladišni patogeni, Apple fruits||, ||Jabuka,

Sažetak

Istraživanja pokazuju da su hitosan i njegovi derivati dobra alternativa u kontroli skladišnih fitopatogenih gljiva. Cilj ovog rada bio je da ispita antifungalnu aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides, izolovanih sa inficiranih plodova jabuke, u in vitro i in situ uslovima. Rast gljiva je testiran in vitro koristeći PDA podlogu sa različitim koncentracijama hitosana (1, 2 i 3 mg/ml). Porast oba patogena je bio značajno smanjen (P<0.05) u svim koncentracijama hitosana nakon 7 dana inkubacije na 25°C. Vodeni rastvor hitosana koncentracije 3 mg/ml je u potpunosti inhibirao klijanje konidija A. alternata i C. gloeosporoides nakon inkubacije od 18 sati na temperaturi od 25°C. Rezultati ogleda in situ ukazuju da inhibicija propadanja plodova inficiranih sa A. alternata i C. gloeosporoides zavisi od koncentracije rastvora hitosana. Pojava bolesti na plodovima jabuka tretiranih rastvorom hitosana nakon 7 dana inkubacije na 25°C bila je značajno smanjena za obe vrste gljiva (P<0.05) u odnosu na pozitivnu kontrolu. Rast A. alternata i C. gloeosporoides je bio progresivno inhibiran in vitro i in situ sa povećanjem koncentracije rastvora hitosana, od 1 do 3 mg/ml.

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Antifungalna aktivnost hitosana prema Alternaria alternata i Colletotrichum gloeosporoides

Sažetak

Reference