Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji

  • Marija Krivokapić Institut za zaštitu bilja i životnu sredinu,Teodora Drajzera 9, 11040 Beograd
  • Veljko Gavrilović Institut za zaštitu bilja i životnu sredinu,Teodora Drajzera 9, 11040 Beograd
  • Milan Ivanović Univerzitet u Beogradu, Poljoprivredni fakultet, Nemanjina 6, Beograd – Zemun
  • Nemanja Kuzmanović Julius Kühn-Institut, Federal Research Centre for Cultivated Plants, Institute for Epidemiology and Pathogen Diagnostics, Messeweg 11-12, Braunschweig
  • Đorđe Fira Univerzitet u Beogradu, Biološki fakultet, Studentski trg 16, Beograd
  • Aleksa Obradović Univerzitet u Beogradu, Poljoprivredni fakultet, Nemanjina 6, Beograd – Zemun
  • Katarina Gašić Institut za zaštitu bilja i životnu sredinu,Teodora Drajzera 9, 1104
DOI: https://doi.org/10.2298/
Ključne reči: Fire blight||, ||Bakteriozna plamenjača, Heterogeneity||, ||Heterogenost, Pathogenicity||, ||Patogenost, RAPD-PCR||, ||RAPD-PCR, Rep-PCR||, ||Rep-PCR, Pome fruits||, ||Jabučaste voćke,

Sažetak


Primenom standardnih bakterioloških i molekularnih metoda proučen je diverzitet 30 sojeva Erwinia amylovora izolovanih iz dunje, kruške i jabuke, poreklom iz 14 lokaliteta u Srbiji. Svi proučavani sojevi izazvali su nekrozu i pojavu bakterijskog eksudata na nesazrelim plodovima kruške, trešnje i šljive, kao i hipersenzitivnu reakciju duvana. Proučavani sojevi bili su Gram i oksidaza negativni, fakultativno anaerobni, levan i katalaza pozitivni i nisu stvarali fluorescentni pigment na Kingovoj podlozi B. Svi sojevi hidrolizuju želatin, koriste citrate i stvaraju kiselinu iz sorbitola, proizvode redukujuće supstance iz saharoze, ne redukuju nitrate, razvijaju se u prisustvu 5% NaCl, ali ne i pri 36°C. Identitet sojeva potvrđen je konvencionalnim PCR i nested PCR metodama. Rep-PCR metodom korišćenjem REP, ERIC i BOX prajmera umnoženo je više fragmenata DNK čiji broj i veličina su se podudarali kod svih proučavanih sojeva. Za razliku od Rep-PCR, primenom RAPD-PCR metode uz korišćenje šest prajmera došlo je do izdavajanja različitih genetičkih profila i diferencijacije sojeva u četiri grupe. Genetičke razlike među proučavanim sojevima nisu bile u korelaciji sa domaćinima iz kojih su izolovani, niti sa njihovim geografskim poreklom i godinom izolacije.

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Objavljeno
2018/12/29
Kako citirati
Krivokapić, M., Gavrilović, V., Ivanović, M., Kuzmanović, N., Fira, Đorđe, Obradović, A., & Gašić, K. (2018). Karakterizacija i diverzitet populacije sojeva Erwinia amylovora poreklom iz jabučastih voćaka gajenih u Srbiji. Pesticides and Phytomedicine / Pesticidi I Fitomedicina, 33(3-4). https://doi.org/10.2298/
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Vol. 33 Br. 3-4 (2018): Pesticidi i fitomedicina
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