Antifiding aktivnost nekoliko biljnih ekstrakata za gusenice Lymantria dispar L. (Lepidopetera: Lymantriidae)

  • Sonja M Gvozdenac Univerzitet u Novi Sadu, Poljoprivredni fakultet, Departman za fitomedicinu i zastitu zivotne sredine
  • Dušanka V Inđić University of Novi Sad, Faculty of Agriculture, Department for plant and environmental protection
  • Slavica M Vuković University of Novi Sad, Faculty of Agriculture, Department for plant and environmental protection
  • Mila S Grahovac University of Novi Sad, Faculty of Agriculture, Department for plant and environmental protection
  • Snežana T Tanasković University of Kragujevac, Faculty of Agronomy Čačak,
Ključne reči: Plant extracts||, ||Biljni ekstrakti, Gypsy moth||, ||Gubar, Insecticides||, ||Insekticidi, Antifeedants||, ||Antifidanti,

Sažetak


Lymantria dispar L. je ekonomski najznačajnija polifagna štetočina listopadnih šuma, voćnjaka i urbanog zelenila. Mehaničke mere i upotreba bioloških insekticida su najčešće primenjivane mere zaštite s ciljem smanjenja šteta koje prouzrokuju gusenice svojom pojavom. Međutim, na manjim površinama, a posebno u godinama gradacije, neizostavna je upotreba konvencionalnih insekticida u zaštiti bilja i šumarstvu. Višegodišnja neodgovarajuća primena ove grupe insekticida je dovela do narušavanja ravnoteže u biocenozi, prenamonženja ranije manje štetnih organizama, pojave rezistentnosti štetnih vrsta na insekticide, kao i prisustva rezidua pesticida u zemljištu i vodotokovima. U cilju smanjenja štetnih posledica neophodno je uskladiti suzbijanje sa principima integralnog pristupa smanjenju brojnosti gubara, primenom selektivih i manje toksičnih insekticida. Naime, sve više se istražuje potencijal botaničkih insekticida i antifiding sredstava. Cilj ovog rada je bio da se proceni uticaj etanolnih ekstraktata Ambrosia artemisiifolia L., Erigeron canadensis L., Daucus carota L., Morus alba L. i Aesculus hippocastanum L. na intenzitet ishrane gusenica L. dispar, kao i njihove antifiding aktivnosti u uslovima “no-choice” testa. Ogled je realizovan u laboratorijskim uslovima. Po 10 larvi po ponavljanju je stavljeno u petri-posude i ponuđena su im po dva isečka lista hrasta (2 x 9 cm2/ponavljanju), potopljena u biljne ekstrakte, odnoso u 1, 2 i 5% rastvor etanola u kontroli. Intenzitet ishrane, iskazan preko procenta konzumirane površine, je meren nakon 48 h. Za procenu antifiding aktivnosti, izračunat je AFI (antifeeding index), a biljni ekstrakti su kategorizovani prema već utvrđenoj skali: nema, slabo, srednje i jako antifiding dejstvo. Podaci su statistički obrađeni dvofaktorijalnom analizom varijanse, uz primenu Dankanovog testa višestrukih poređenja. Rezultati rada ukazuju da je značajan uticaj na konzumiranu površinu lista imalo poreklo biljnog ekstrakta, dok koncentracija i interakcija dva faktora (biljna vrsta x koncentracija), nisu statistički značajno uticale na vrednost konzumirane površine. Ekstrakti Ae. hippocastanum i M. alba statistički su značajno smanjili procenat konzumirane površine (6.24, 18.93%, respektivno) u poređenju sa kontrolom (97.59%), dok je ekstrakt D. carota ispoljio fagostimulativni efekat (98.88%). Na osnovu AFI vrednosti, ekstrakt Ae. hippocastanum (87.10-89.05%) je ispoljio jaku antifiding aktivnost, a M. alba srednju do jaku (64.33-71.37%).

 

 

Reference

Anonymous: Pharmacopoedia Jugoslavica. Editio quarta (Ph. Jug. IV), Savezni zavod za zdravstvenu zaštitu, Beograd, 1984.

Barbosa, P.P. and Krischik, V.: Influence of alkaloids on feeding preference of eastern 299 decidious forest trees by the gypsy moth Lymantria dispar. American Naturalist, 130, 53-69, 1987.

Bernays, E. and Chapman, R.F.: Host-plant selection by phytophagous Chapman and Hall, New York, 117-148. 1994.

Bočarov-Stančić, A., Nikolić, N., Štrbac, S., Bijelić, M., Tabaković-Tošić, M. and Momirov, R.: Preliminary investigations of the compatibility of biolarvicide D-STOP SC with fungicide Benfungin WP, Proceedings of the symposium "Biocenose regulation as a background for updated phytosanitary technologies" In: Information Bulletin EPRS IOBC. Ed. by the All-Russian Institute of Plant Protection (VIZR), May 21-25, St. Petersburg, Russia, 2007, 38, pp.44-47.

Bočarov-Stančić, A., Vuković, S., Slavić, D. and Gološin, B.: Biological insecticides in safe food production. Scientific work, Eco-conference, Novi Sad, Serbia, September 25-28, 2002, pp.353-358.

Cao, Y., Cui, Z.,Wang, Y. and Chen, W.: Effects of plant alcohol extracts on Diaeretietta rapae, Hubei. Agricultural Science and Rewiew, 4, 2004.

Doskotch, R.W., Cheng, H.Y., Odell, T.M. and Girarad, L.: Neolidol: An antifeeding sesquiterpene alcohol for gypsy moth larvae from Mehdeuca leucadendron. Journal of Chemical Ecology, 6, 845-851, 1980.

Erturk, O., Sekeroglu, V., Unal, H. and Arslan, H.: Antifeedant and toxicity effects of some plant extracts on Lymantria dispar L. (Lep: Lymntridae). Journal of the Faculty of Agriculture OMU, 21, (3), 289-295, 2006.

Gvozdenac, S., Inđić, D., Tabaković-Tošić, M., Vuković, S. and Grahovac, M. Afinitet gusenica gubara (Lymantria dispar L.) prema primarnim i potencijalnim domaćinima. X Savetovanje o zaštiti bilja, 29.11- 03.12., Zlatibor, Serbia, 2010, Zbornik rezimea radova, str. 51.

Gvozdenac, S., Inđić, D., Vuković, S., Grahovac, M., Inđić, I., Tanasković, S. and Sretenović, D. (2011). Antifeedant potential of plant extracts on Lymantria dispar L. (Lymantriidae) larvae. 7th MGPR International Symposium „Paolo Cabras“ Pesticides in food and the environment in Mediterranean countries, September 09-11, Thessaloniki, Greece, 2011, Abstracts, pp. 77.

Hagen, D.C. : Inviting Caterpillars Into Your Garden

Ivanović, I. and Nenadović, V.: Značaj fizioloških istraživanja za proučavanje uzroka prenamnoženja gubara Lymantria dispar L. Gradacije gubara u Srbiji. Entomološko društvo Srbije i Šumarski fakultet Beograd, 73-88, 1998.

Janković, LJ.: Prilog poznavanju biljki hraniteljki gubara u periodu u toku poslednje gradacije (1953-1958). Zaštita bilja, 49/50, 36-39, 1958.

Kamaraj, C., Rahuman, A.A. and Bagavan, A.: Antifeedant and larvicidal effects of plant extracts against Spodoptera litura (F.), Aedes aegypti L., and Culex quinquefasciatus Say. Parastiology Research, 103, 325-331, 2008.

Kaushik, G., Satya, S. and Naik, S.N.: Food procesing a tool to pesticide resuludes dissipation-a review. Food Research International, 42, 26-40, 2009.

Kostić, M., Popović, Z., Brkić, D. and Milanović, S.: Larvicidal and antifeedant activity of some plant-derived compounds to Lymantria dispar L. (Lepidoptera: Lymantriidae). Biosource technology, 99, 7897-7901, 2008.

Lazarević, J., Perić-Mataruga, V., Stojković, B. and Tucić, N.: Adaption of the gypsy moth to an unsuitable host plant. Entomologia Experimentalis et Applicata, 102, 75-86, 2002.

Liu Zhi Long, Swee Hock Goh, Shuit Hung Ho: Screening of Chinese medicinal herbs for bioactivity against Sitophilus zeamais Motschulsky and Tribolium castaneum (Herbst). Journal of Stored Products Research, 43, 290–296, 2007.

Mansson, P.E.: Host selection and antifeedants in Hylobius abietis pine weevils. PhD thesis, Swedish university of agricultural sciences, Alnarp, 33, 2005.

Marković, I., Norris, D.M. and Cekić, M.: Some chemical bases for gypsy moth Limantria dispar, larval rejection of green ash, Fraxinus pennsylvanica, foliage as food. Journal of Chemical Ecology, vol 22, 12, 1996.

McEwan, R.W. and Rieske, L.K.: Potential interactions between invasive woodyshrubs and the gypsy moth (Lymantria dispar), an invasive insect herbivore. Biological Invasions, 11, 1053-1058, 2009.

Mihajlović, LJ.: Gubar (Lymantria dispar L.) (Lepidoptera, Lymantridae) u Srbiji. Šumarstvo, vol 60, 1-2, 1-26, 2008.

Miller, J.C. and Hanson, P.E. : Laboratory feeding tests on the development of L.dispar larvae with reference to plant taxa and allelochemicals. Agricultural Experiment Station. Ir; Oregon State, University, 1994. http://hdl.handle.net/1957/15856. Datum pristupa stranici: 20.05.2012.

Milanović, S., Tabaković-Tošić, M. and Marković, N.: Effect og host plant on gypsy moth diet and biological efficacy of BTK preparations. Glasnik šumarskog fakulteta, 94, 197-210, 2006.

Parkash, A. and Rao, J.: Botanical pesticides in agriculture. CRC Press Inc, 461, 1997.

Pavela, R. and Chermenskaya, T.: Potential insecticidal activity of extracts from 18 species of medicinal plants on larvae of Spodoptera littoralis. Plant Protection Science, 4, 145-150, 2004.

Pavela, R.: Antifeedant activity of some plant extracts on Leptinotarsa decemlineata Say. and Spodoptera littoralis. Bois. larvae. Industrial Crops and Products, 32, 213-219, 2010.

Pelletier, S.: Alkaloids: chemical and biological perspectives, Tom 15, 629, 1996.

Rugutt, K.J., Fronczek, F.R., Franzblau, S.G. and Warner, I.M.: Dihydroparthenolidediol, a novel sesquiterpene lactone. Acta crystallographica section E, 57, 23- 325, 2001.

Shields, V.D.C., Rodgers, E.J., Arnold, N.S. and Williams, D.: Feeding responses to selected alkaloids by gypsy moth larvae, Lymantria dispar (L.). Naturwissenschaften, 93, 127–130, 2006.

Simmonds, M.J.S.: Molecular- and chemo-systematics: do they have a role in agrochemical discovery. Crop protection, 19, 591-596, 2000.

Sretenović, D.: Efekat piretrina (Pyros) u suzbijanju Lymantria dispar L. (Lepidoptera: Lymantriidae). Specijalistički rad, Agronomski fakultet, Čačak, 33, 2007.

Tabaković-Tošić, M.: Possibility of synergetic effect of biological and chemical insecticides in the control of gypsy moth outbreak. Šumarstvo, 4, 71-78, 2005.

Tabaković-Tošić, M.: Management possibilities of gypsy moth muliplication in forest ecosystems of protected areas. Scientific conference: „ L. dispar managment of forest ecosystems in national parksa and other proetected areas“, Jahorina, BIH, July 05-08. 2006, Proceedings, pp. 373-379.

Tabaković-Tošić, M.: Integrated forest protection in forestry of the republic of Serbia. International Scientific conference in occasion of 60 year of operation of Institute of forestry, Belgrade, Serbia, Sustainable use of forest ecosystems- The challege for the 21st century. Donji Milanovac, Serbia, November 8-10, 2006a, Proceedings, pp. 265-273

Tabaković-Tošić, M.: Compatibility of soyabean oil and biological insecticide D-STOP in the synchronized suppression of oak mildew and the gypsy moth. Information builten, Sankt-Petreburg, 226-230, 2007.

Tamura, Y., Hattori, M., Konno, K., Honda, H. and Kono, Y.: Relationship between the host plant preference of the leaf beetle Ophraella communa LeSage (Coleoptera: Chrysomelidae) and distribution of feeding stimulants in asteraceous plants. Japanese Journal of Applied Entomology and Zoology, Vol 48, 3, 191-199, 2004.

Taut, I., Simonica, V. and Rob, M.: Preliminaries research concerning Limantria dispar defoliator present in the beech forests. Bulletin USAMV-CN, 64, 2007.

Tvedten, S.: Safe and far more effective pest control overview. http://www.stephentvedten.com/11_Basic_Control_Overview.pdf, 2006. Datum pristupa stranici: 23.03.2012.

Vuković, S., Tabaković-Tošić, M., Nađalin, V., Đurić, T. and Gološin, B.: Nov pristup u suzbijanju dudovca (Hyphantria cunea) na voću i gubara (Lymantria dispar) u šumama primenom bioinsekticida D-STOP na bazi Bacillus thiringiensis var Kurstaki. XII simpozijum o zaštiti bilja i savetovanje o primeni insekticida, Zlatibor, Serbia, November 25-29, 2002, Abstract book, str. 139.

Zabel, A., Manojlović, B., Stanković, S., Tomaši, O., Kostić, M.: Delovanje etarskog ulja Myristi-ca fragrans Hout na Leptinotarsa decemlineata Say. i Lymantria dispar L. Zaštita bilja, 211- 234, 2000.

Zabel, A., Manojlović, B., Rajković, S., Stanković, S. and Kostić, M.: Effect of neem extract on Lymantria dispar L. (Lepidoptera: Lymantriidae) and Leptinotarsa decemlineata (Coleoptera: Chrisomelidae). Journal of Pesticide Science, 75, 19-25, 2002.

Objavljeno
2013/02/12
Rubrika
Originalni naučni članak