Pojava i molekularna karakterizacija virusa crtičastog mozaika pšenice na pšenici u Srbiji

  • Ana Vučurović Univerzitet u Beogradu, Poljoprivredni fakultet, Nemanjina 6, 11080 Beograd https://orcid.org/0000-0002-9803-6700
  • Ivana Stanković Institute of Phytomedicine, Department of Phytopathology, University of Belgrade-Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
  • Katarina Zečević Institute of Phytomedicine, Department of Phytopathology, University of Belgrade-Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
  • Branka Petrović Institute of Phytomedicine, Department of Phytopathology, University of Belgrade-Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
  • Goran Delibašić Institute of Phytomedicine, Department of Phytopathology, University of Belgrade-Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
  • Branka Krstić Institute of Phytomedicine, Department of Phytopathology, University of Belgrade-Faculty of Agriculture, Nemanjina 6, 11080 Belgrade, Serbia
DOI: https://doi.org/10.2298/PIF2002117V
Ključne reči: virus crtičastog mozaika pšenice, pšenica, RT-PCR, molekularna karakterizacija, filogenija, Srbija

Sažetak


Virus crtičastog mozaika pšenice (Wheat streak mosaic virus, WSMV) je široko rasprostranjen, prenosi se grinjom kovrdžanja pšenice i nanosi štete u proizvodnji pšenice u mnogim regionima sveta. Nakon prve detekcije u Srbiji 60-ih godina XX veka, prisustvo ovog virusa nije praćeno. Tokom 2019. godine, sakupljeno je ukupno 62 uzorka pet sorti pšenice poreklom sa osam lokaliteta u Srbiji koji su testirani na prisustvo devet najznačajnijih virusa pšenice: WSMV, virus žute patuljavosti ječma-PAV, -MAV -SGV i -RMV (barley yellow dwarf virus-PAV, -MAV, -SGV i -RMV [BYDV-PAV, -MAV, -SGV i -RMV]), virus žute patuljavosti žitarica-RPV (cereal yellow dwarf virus-RPV, CYDV-RPV), virus vretenastog crtičastog mozaika pšenice (wheat spindle streak mosaic virus, WSSMV), virus mozaika ovsika (brome mosaic virus, BMV) i virus mozaika pšenice koji se prenosi zemljišnim pseudogljivama (soil-borne wheat mosaic virus, SBWMV) primenom RT-PCR ili multiplex RT-PCR. Prisustvo WSMV dokazano je u 58,1% testiranih uzoraka pšenice sakupljenih u sedam useva na pet različitih lokaliteta, dok prisustvo drugih testiranih virusa nije dokazano ni u jednom od testiranih uzoraka. U cilju dalje identifi kacije, umnožavanje i sekvenciranje gena za proteinski omotač (coat protein, CP gen) obavljeno je primenom RT-PCR metode uz korišćenje WS8166F/WS8909R prajmera. Amplifikovani fragmenti odgovarajuće veličine (750 bp) četiri odabrana izolata: 98-19, 99-19, 102-19 i 120-19 su poslati na sekvenciranje, a dobijene sekvence su deponovane u GenBank bazu podataka (MT461299, MT461300, MT461301 i MT461302). Izolati WSMV poreklom iz Srbije pokazuju visok stepen nukleotidne identičnosti (98,16-99,02) i deleciju GCA kodona na poziciji 8412-8414 koja dovodi do delecije aminokiseline glicin (Gly2761). Filogenetska analiza, na osnovu sekvence CP gena, pokazala je grupisanje odabranih izolata u četiri podgrupe, A, B, C i D, i jedne nedavno izdvojene podgrupe B1. Izolati WSMV iz pšenice poreklom iz Srbije grupisali su se u podgrupu B zajedno sa ostalim izolatima iz Evrope i jednim izolatom iz Irana. Rezultati ovih istraživanja daju prvi uvid u molekularnu karakterizaciju izolata WSMV poreklom iz Srbije, ukazujući na blisku evolutivnu povezanost izolata iz Srbije sa drugim evropskim izolatima i postojanje jedinstvenog genotipa u našoj zemlji. Takođe, fi logenetske analize ukazuju na širenje izolata WSMV u Evropi iz jednog centra porekla.

 

Reference

Appel, J.A., Dewolf, E., Todd, T., & Bockus, W.W. (2014). Preliminary 2015 Kansas wheat disease loss estimates. Kansas Department of Agriculture Annual Disease Survey Report. Topeka, KS: Plant Health Division. Retrieved from https://agriculture.ks.gov/docs/default-source/pp-disease-reports-2012/2015-ks-wheat-isease-loss-estimates35b3d4002e6262e1aa5bff 0000620720.pdf ?sfvrsn=0

Atkinson, T.G., & Grant, M.N. (1967). An evaluation of streak mosaic losses in winter wheat. Phytopathology, 57(2), 188-192.

Bennypaul, H., Abdullahi, I., Harding, M. W., & Aboukhaddour, R. (2019). First detection of European isolates of Wheat streak mosaic virus in Canada. Plant Disease, 103(6), 1442. doi: 10.1094/pdis-12-18-2193-pdn

Brunt, A.A., Crabtree, K., Dallwitz, M., Gibbs, A., & Watson, L. (1996). Viruses of plants. Descriptions and lists fr om the VIDE database. Wallingford, UK: CAB International.

Burrows, M., Franc, G., Rush, C., Blunt, T., Ito, D., Kinzer, K. ... Ziems, A. (2009). Occurrence of viruses in wheat in the Great Plains region, 2008. Plant Health Progress, 10(1), 14. doi: 10.1094/PHP-2009-0706-01-RS

Byamukama, E., Seifers, D.L., Hein, G.L., De Wolf, E., Tisserat, N.A., Langham, M.A.C. … Wegulo, S.N. (2013). Occurrence and distribution of Triticum mosaic virus in the central Great Plains. Plant Disease, 97(1), 21-29. doi: 10.1094/PDIS-06-12-0535-RE

Chalupníková, J., Kundu, J.K., Singh, K., Bartaková, P., & Beoni, E. (2017). Wheat streak mosaic virus: incidence in fi eld crops, potential reservoir within grass species and uptake in winter wheat cultivars. Journal of Integrative Agriculture, 16(3), 523-531. doi: 10.1016/S2095-3119(16)61486-7

Deb, M., & Anderson, J. M. (2008). Development of a multiplexed PCR detection method for Barley and Cereal yellow dwarf viruses, Wheat spindle streak virus, Wheat streak mosaic virus and Soilborne wheat mosaic virus. Journal of Virological Methods, 148(1-2), 17-24. doi: 10.1016/j.jviromet.2007.10.015

Dráb, T., Svobodová, E., Ripl, J., Jarošová, J., Rabenstein, F., Melcher, U., & Kundu, J.K. (2014). SYBR Green I based RT-qPCR assays for the detection of RNA viruses of cereals and grasses. Crop and Pasture Science, 65(12), 1323-1328. doi: 10.1071/CP14151

French, R., & Stenger, D.C. (2002). Wheat streak mosaic virus. CMI/AAB Descriptions of Plant Viruses. No. 393. Wellesbourne, UK: Association of Applied Biologists.

Gadiou, S., Kudela, O., Ripl, J., Rabenstein, F., Kundu, J. K., & Glasa, M. (2009). An amino acid deletion in Wheat streak mosaic virus capsid protein distinguishes a homogeneous group of European isolates and facilitates their specifi c detection. Plant Disease, 93(11), 1209-1213. doi: 10.1094/PDIS-93-11-1209

Hadi, B.A.R., Langham, M.A.C., Osborne, L., & Tilmon, K.J. (2011). Wheat streak mosaic virus on wheat: Biology and management. Journal of Integrated Pest Management, 2(1), 1-5. doi: 10.1603/IPM10017

Hunger, R.M. (2010). Wheat streak mosaic virus. In: W.W. Bockus, R.L. Bowden, R.M. Hunger, W.L. Morrill, T.D. Murray and R.W. Smiley (eds.), Compendium of wheat diseases and pests, 3rd edition (pp 115-117). St. Paul, Minnesota, USA: American Phytopathological Society Press.

Jones, R.A.C., Coutts, B.A., Mackie, A.E., & Dwyer, G.I. (2005). Seed transmission of Wheat streak mosaic virus shown unequivocally in wheat. Plant Disease, 89(10), 1048-1050. doi: 10.1094/PD-89-1048

Krstić, B., Stanković, I., & Vučurović, A. (2018). Viroze ratarskog, povrtarskog i ukrasnog bilja (Viruses of field, vegetable and ornamental plants). Belgrade, Serbia: University of Belgrade, Faculty of Agriculture. (in Serbian).

Kúdela, O., Kúdelová, M., Nováková, S., & Glasa, M. (2008). First report of Wheat streak mosaic virus in Slovakia. Plant Disease, 92(9), 1365. doi: 10.1094/PDIS-92-9-1365C

Kumar, S., Stecher, G., Li, M., Knyaz, C., & Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35(6), 1547-1549. doi: 10.1093/molbev/msy096

Lanoiselet, V.M., Hind-Lanoiselet, T.L., & Murray, G.M. (2008). Studies on the seed transmission of Wheat streak mosaic virus. Australasian Plant Pathology, 37(6), 584-588. doi: 10.1071/AP08059

Lapierre, H., & Signoret, P.A., eds. (2004). Viruses and virus diseases of Poaceae (Gramineae). Paris, France: INRA.

Li, R., Mock, R., Huang, Q., Abad, J., Hartung, J., & Kinard, G. (2008). A reliable and inexpensive method of nucleic acid extraction for the PCR based detection of diverse plant pathogens. Journal of Virological Methods, 154(1-2), 48-55. doi: 10.1016/j.jviromet.2008.09.008

Lucas, H. (2012). Breakout session P1.1 National Food Security-speaker brief-the wheat initiativean international research initiative for wheat improvement. In Second Global Conference on Agricultural Research for Development (GCARD2), Punta del Este, Uruguay (pp. 1-3). Retrieved from https://www.gfar.net/documents/gcard2-breakout-session-p11-national-food-securityspeaker-brief-wheat-initiative

McKinney, H.H. (1937). Mosaic diseases of wheat and related cereals. US Department of Agriculture Circular, 442, 1-23.

Mishchenko, L.T., Dunich, A.A., Skrypkina, I.Y., & Kozub, N.O. (2019). Phylogenetic analysis of two Ukrainian isolates of Wheat streak mosaic virus. Вiopolymers and Cell, 35(1), 64-77. doi: 10.7124/bc.000997

Navia, D., de Mendonca, R.S., Skoracka, A., Szydło, W., Knihinicki, D., Hein, G.L. ... Lau, D. (2013). Wheat curl mite, Aceria tosichella, and transmitted viruses: An expanding pest complex aff ecting cereal crops. Experimental and Applied Acarology, 59(1-2), 95-143. doi: 10.1007/s10493-012-9633-y

Ordon, F., Habekuss, A., Kastirr, U., Rabenstein, F., & Kühne, T. (2009). Virus resistance in cereals: sources of resistance, genetics and breeding. Journal of Phytopathology, 157(9), 535-545. doi: 10.1111/j.1439-0434.2009.01540.x

Price, J.A., Workneh, F., Evett, S.R., Jones, D.C., Arthur, J., & Rush, C.M. (2010). Eff ects of Wheat streak mosaic virus on root development and water-use efficiency of hard red winter wheat. Plant Disease, 94(6), 766-770. doi: 10.1094/PDIS-94-6-0766

Rabenstein, F., Seifers, D.L., Schubert, J., French, R., & Stenger, D.C. (2002). Phylogenetic relationships, strain diversity and biogeography of tritimoviruses. Journal of General Virology, 83(4), 895-906. doi: 10.1099/0022-1317-83-4-895

Roberts, A.G. (2014). Plant Viruses: Soilborne. eLS. Encyclopedia of Life Sciences. Chichester, UK: John Wiley & Sons, Ltd. doi: 10.1002/9780470015902.a0000761.pub3

Robinson, M.D., & Murray, T.D. (2013). Genetic variation of Wheat streak mosaic virus in the United States Pacifi c Northwest. Phytopathology, 103(1), 98-104. doi: 10.1094/PHYTO-05-12-

-R

RZS (2019). Statistički godišnjak biljne proizvodnje (Statistical annual bulletin of plant production). Retrived from Republički zavod za statistiku, Republika Srbija (Statistical Offi ce of the Republic of Serbia), https://publikacije.stat.gov.rs/G2019/Pdf/G20192052.pdf

Schubert, J., Ziegler, A. & Rabenstein, F. (2015). First detection of wheat streak mosaic virus in Germany: molecular and biological characteristics. Archives of Virology, 160, 1761–1766. doi: 10.1007/s00705-015-2422-2

Seifers, D.L., Martin, T.J., Harvey, T.L., Fellers, J.P., Stack, J.P., Ryba-White, M. ... Standing, K.G. (2008). Triticum mosaic virus: A new virus isolated from wheat in Kansas. Plant Disease, 92(5), 808-817. doi: 10.1094/PDIS-92-5-0808

Šeremešić, S., Ćirić, V., Milošev, D., Vasin, J., & Djalović, I. (2017). Changes in soil carbon stock under the wheat-based cropping systems at Vojvodina province of Serbia. Archives of Agronomy and Soil Science, 63(3), 388-402. doi: 10.1080/03650340.2016.1218475

Singh, K., & Kundu, J.K. (2017). Variations in coat protein sequence of Wheat streak mosaic virus among crop and non-crop hosts. Crop and Pasture Science, 68(4), 328-336. doi: 10.1071/CP17025

Singh, K., & Kundu, J.K. (2018). Wheat streak mosaic virus: Cereal pathogen with growing importance. In R. Kumar Gaur, S.M.P Khurana & Y. Dorokhov (Eds.), Plant viruses-Diversity, interaction and management (pp 131-148). Florida, USA: CRC Press.

Singh, K., Wegulo, S.N., Skoracka, A., & Kundu, J.K. (2018). Wheat streak mosaic virus: a century old virus with rising importance worldwide. Molecular Plant Pathology, 19(9), 2193-2206. doi: 10.1111/mpp.12683

Skoracka, A., Rector, B., Kuczyński, L.,Szydło, W., Hein, G., & French, R. (2014). Global spread of wheat curl mite by its most polyphagous and pestiferous lineages. Annals of Applied Biology, 165(2), 222-235. doi: 10.1111/aab.12130

Spaar, D., Ordon, F., Rabenstein, F., Habekuß, A., Schliephake, E., & Schubert, J. (2008). Economic significance and incidence of cereal viruses in Germany and possibilities to avoid virus caused yield losses. Vestnik zascity rastenii = Plant Protection News, 1(1), 14-26.

Stanković, I., Zečević, K., Vučurović, A., Petrović, B., Ristić, D., Vučurović, I. ... Krstić, B. (2019). Zastupljenost i diverzitet virusa pšenice u Srbiji (Occurrence and diversity of wheat viruses in Serbia). In Zbornik rezimea 16. Simpozijuma o zaštiti bilja (Book of Abstracts of 16th Plant Protection Symposium) (pp 37-38). Mostar, Bosnia and Herzegovina: Plant Protection Society of Bosnia and Herzegovina.

Stenger, D.C., & French, R. (2009). Wheat streak mosaic virus genotypes introduced to Argentina are closely related to isolates from the American Pacifi c Northwest and Australia. Archives of Virology, 154(2), 331-336. doi: 10.1007/s00705-008-0297-1

Šutić, D., & Tošić, M. (1964). Virus crtičastog mozaika pšenice u našoj zemlji (Wheat streak mosaic virus in Yugoslavia). Zaštita bilja, 79, 307-314.

Šutić, D., & Tošić, M. (1966). Recent investigations of wheat virus diseases in Yugoslavia. Savremena poljoprivreda, 11-12, 385-393.

Thompson, J.D., Higgins, D.G., & Gibson, T.J. (1994). CLUSTAL W: Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Research, 22, 4673-4680. doi: 10.1093/nar/22.22.4673

Tošić, M. (1971). Virus diseases of wheat in Serbia: I. Isolation and determination of the Wheat streak mosaic virus and Brome mosaic virus. Journal of Phytopathology, 70(2), 145-162. doi: 10.1111/j.1439-0434.1971.tb03472.x

Trębicki, P., Nancarrow, N., Cole, E., Bosque-Pérez, N.A., Constable, F.E., Freeman, A.J. ... Fitzgerald, G.J. (2015). Virus disease in wheat predicted to increase with a changing climate. Global Change Biology, 21(9), 3511-3519. doi: 10.1111/gcb.12941

Trzmiel, K., Zarzyńska-Nowak, A., Lewandowska, M., & Szydło, W. (2016). Identifi cation of new Brome mosaic virus (BMV) isolates systemically infecting Vigna unguiculata L. European Journal of Plant Pathology, 145(1), 233-238. doi: 10.1007/s10658-015-0830-5

Vacke, J., Zacha, V., & Jokeš, M. (1986). Identification of virus in wheat new to Czechoslovakia. In Proceeding of X Czechoslovak Plant Protection Conference (pp 209-210). Brno, Czech Republic.

Velandia, M., Rejesus, R.M., Jones, D.C., Price, J.A., Workneh, F., & Rush, C.M. (2010). Economic impact of Wheat streak mosaic virus in the Texas High Plains. Crop Protection, 29(7), 699-703. doi: 10.1016/j.cropro.2010.02.005

Workneh, F., Jones, D.C., & Rush, C.M. (2009). Quantifying wheat yield across the fi eld as a function of Wheat streak mosaic intensity: a state space approach. Phytopathology, 99(4):432-440. doi: 10.1094/PHYTO-99-4-0432

Objavljeno
2020/12/17
Kako citirati
Vučurović, A., Stanković, I., Katarina Zečević, Petrović, B., Delibašić, G., & Krstić, B. (2020). Pojava i molekularna karakterizacija virusa crtičastog mozaika pšenice na pšenici u Srbiji. Pesticides and Phytomedicine / Pesticidi I Fitomedicina, 35(2), 117-131. https://doi.org/10.2298/PIF2002117V
Broj časopisa
Vol. 35 Br. 2 (2020): Pesticidi i fitomedicina
Rubrika
Originalni naučni članak
Autori koji objavljuju u ovom časopisu pristaju na sledeće uslove:

  1. Autori zadržavaju autorska prava i pružaju časopisu pravo prvog objavljivanja rada i licenciraju ga "Creative Commons Attribution licencom" koja omogućava drugima da dele rad, uz uslov navođenja autorstva i izvornog objavljivanja u ovom časopisu.

  2. Autori mogu izraditi zasebne, ugovorne aranžmane za neekskluzivnu distribuciju članka objavljenog u časopisu (npr. postavljanje u institucionalni repozitorijum ili objavljivanje u knjizi), uz navođenje da je članak izvorno objavljen u ovom časopisu.

  3. Autorima je dozvoljeno i podstiču se da postave objavljeni članak onlajn (npr. u institucionalni repozitorijum ili na svoju internet stranicu) pre ili tokom postupka prijave rukopisa, s obzirom da takav postupak može voditi produktivnoj razmeni ideja i ranijoj i većoj citiranosti objavljenog članka (Vidi Efekti otvorenog pristupa).