Fitotoksičnost hlorpirifosa za slačicu (Sinapis alba L.) i kukuruz (Zea mays L.): potencijalne indikatore prisustva insekticida u vodi
Sažetak
Insekticid hlopririfos prema Direktivi 2008/105/EC svrstan je među prioritetne polutante vode i takođe značajan polutant životne sredine. Njegovo prisustvo se detektuje uglavnom hemijskim metodama, međutim biološki testovi sve više dobijaju na značaju u poslednjih nekoliko godina te je cilj ovog rada bila procena potencijala bele slačice (Sinapis alba L.) i kukuruza (Zea mays L.) kao bioindikatora kontaminacije vode. Fitotoksični efekti hlopririfosa (količine 0,05-10µg a.m./l vode) su procenjeni preko fizioloških (energija klijanja i klijavost) i morfoloških parametara (dužina korena i izdanka, sveža i suva masa korena i izdanka) ispitivanih vrsta. Korišćena je modifikovana metoda na filtar hartiji. Podaci su obrađeni Dankanovim testom višestrukih poređenja i Probit analiza pri određivanju toksičnosti (EC50). Prema pomenutoj Direktivi, maksimalno dozvoljena količina (MAC) hlorpirofosa u vodi je 0,1µg a.m./l vode. Pri primeni hlorpirifosa u MAC količini, energija klijanja i klijavost semena bele slačice (11,25%) su bile značajno inhibirane u poređenju sa kontrolom (91,5; 93,5%) dok je formiranje hipokotila i epikotila izostalo. U količini 50% nižoj do MAC (0,05 µg a.m. /l), energija klijanja i klijavost (87,75; 88,25%) su u poređenju sa kontrolom bile značajno smanjene, kao i dužina korena i izdnka ponika. Hlorpirifos nije uticao na energiju klijanja i klijavost semena kukuruza, dok su morfološki parametri bili značajno smanjeni već pri primeni inskticida u količini od 0,1µg a.m./l. Toksičnost hlorpirifosa, to jest EC50 za klijavost semena bele slačice je iznosila 0,09 µg a.m./l, a za kukuruz 3,21 µg a.m./l vode.
Reference
Anonymous 1. (2012). http://en.wikipedia.org/wiki/ChlorpyrifosAnonimous
Anonymous 2. U.S. Environmental Protection Agency (1989): Registration Standard (Second Round Review) for the Registration of Pesticide Products Containing Chlorpyrifos Washington DC, 5-44.
Banks, K. E., Hunter, D. H., Wachal, D. J. (2005). Chlorpyrifos in surface waters before and after a federally mandated ban. Environ. Int. 31(3), 351-356.
Boutin, C., Elmegaard, N., Kjær, C. (2004). Toxicity testing of fifteen noncrop plant species with six herbicides in a green house experiment: implications for risk assessment. Ecotoxicology, 23, 34-369.
Cerejeira, M. J., Viana, P., Batista, S., Pereira, T., Silva, E., Valério, M. J., Silva, A., Ferreira, M., Silva-Fernandes, A. M. (2003). Pesticides in Portuguese surface and ground waters. Water Res., 37(5), 1055-1063.
Chapman, E., Dave, G., Murimboh, J. (2010). Ecotoxicological risk assessment of undistrubed metal contaminated soil at two remote lighthouse sites. Ecotoxicology and environmental safety, 73, 961-969.
Chu Xiaoqiang, Fang Hua, Pan Xuedong, Wang Xiao, Shan Min, Feng Bo , Yu Yunlong (2008). Degradation of chlorpyrifos alone and in combination with chlorothalonil and their effects on soil microbial populations. Journal of Environmental Sciences, 20(4), 464-469.
Dubey, K. K., Fulekar, M. H. (2011). Effect of pesticides on the Seed Germination of Cenchrus setigerus and Pennisetum pedicellatum as Monocropping and Co-cropping System: Implications for Rhizospheric Bioremediation. Romania Biotechnological Letters, 16(1), 5909-5918.
Janjić, V., Elezović, I. (2010). Pesticides in agriculture and forestry in Serbia. Ed Plant protection Society of Serbia, Belgrade, Serbia
Klokočar-Šmit, Z., Inđić, D. (1991). Negative effects of seed treatment. 4th Monography „Seed apotheosis“ (Production of quality seeds of small grains and maize). MRAZ; Seme-Sombor, Sombor; Institut za ratarstvo i povrtarstvo, Novi Sad; Institut za zaštitu bilja, Novi Sad; Institut za poljoprivrednu tehniku, Novi Sad; Vojvođansko društvo za poljoprivrednu tehniku, Novi Sad, 107-113.
Li Chun-Xi, Feng Shu-Li, Shao Yun, Jiang Li-Na, Lu Xu-Yang, Hou Xiao-Li (2007). Effects of arsenic on seed germination and physiological activities of wheat seedlings. Journal of Environmental Sciences, 19, 725-732.
McEwen, F. L., Stephenson, G. R. (1979). The Use and Significance of Pesticides in the Environment. John Wiley and Sons, New York
Mitić, M. (1982). Pesticides in agriculture and forestry in Yugoslavia. 4th Edition. National Agricultural Committee, Belgrade
Montagna, M. C., Collins, P. A. (2007). Survival and growth of Palaemonetes argentinus
(Decapoda; Caridea) exposed to insecticides with chlorpyrifos and endosulfan as active
element. Archives of Environmental Contamination and Toxicology, 53(3), 371-378.
Moore, M. T., Kröger, R. (2010). Effect of three insecticides and two herbicides on rice
(Oryza sativa L.) seedling germination and growth. Arch. Environ. Contam. Toxicol.,
(4), 574-581.
OECD Guideline for Testing of Chemicals 208 (2003). Terrestrial Plants, Growth Test
O’Halloran, K. (2006). Toxicological considerations of contaminants in the terrestrial environment for ecological risk assessment. Hum. Ecol. Risk Assess, 12(1), 74-83.
Palma, P., Palma, V. L., Fernandes, R. M., Soares, A. M. V. M., Barbosa, I. R. (2008). Acute toxicity of atrazine, endosulfan sulphate and chlorpyrifos to Vibrio fischeri, Thamnocephalus platyurus and Daphnia magna, relative to their concentrations in surface waters from the Alentejo region of Portugal. Bull. Environ. Contam. Toxicol., 81(5), 485-489.
Pascoe, G. A. (1993). Wetland risk assessment. Environmental Toxicology and Chemistry, 12(12), 2293-2307.
Regulation on the quality of seeds of agricultural plants, Official gazette SRJ 58/2002
Rubach, M. N., Baird, D. J., Boerwinkel, M. C., Maund, S. J., Roessink, I. (2012). Species traits as predictors for intrinsic sensitivity of aquatic invertebrates to the insecticide chlorpyrifos. Ecotoxicology, 21(7), 2088-2101.
Schulz, R., Liess, M. (1999). A field study of the effects of agricultural derived insecticide input on stream macroinvertebrate dynamics. Aquat. Toxicol., 46, 155-176.
Sekulić, J., Jeličić, N. (2013): Pesticidi u prometu u Srbiji. Plant doctor, Belgrade
Shafiq-ur-Rehman, Shaheen Rehman, M., Waliullah, S. (2012). Chlorpyrifos-induced Neuro-Oxidative Damage in Bee. Toxicology and Environmental Health Sciences, 4(1), 30-36.
Sperone, E., Tripepi, S., Brunelli, E. (2011). Toxicity of Chlorpyrifos to Larval Rana dalmatina: Acute and Chronic Effects on Survival, Development, Growth and Gill Apparatus. Ilaria Bernabò, Archives of Environmental Contamination and Toxicology, 61(4), 704-718.
Stevanović, V., Inđić, D. , Knežević, B. (2009a). The effect of fungicides for seed treatment on germination of barely. Pesticides and phytomedicine, 24(1), 35-41.
Stevanović, V., Knežević, B., Inđić, D. (2009b): The Effect of seed fungicide treatment on germination of barely. Plant protection, 20, 70-75.
Straw, N. A., Fielding, N. J., Waters, A. (1996). Phytotoxicity of insecticides used to control aphids on Sitka spruce, Picea sitchensis (Bong) Carr. Crop Protection, 15(5), 451-459.
Tongbai, W., Boonplueng, R., Damrongphol, P. (2012). Enzymatic responses of the riceland prawn, Macrobrachium lanchesteri to chlorpyrifos exposure. Biologia, 67(4), 762-766.
Wang, L., Jiang, X., Wang, D. (2007). Behavior and fate of Chlorpyrifos introduced into soil-crop systems by irrigation. Chemosphere, 66(3), 391-396.
White, A. L., Boutin, C. (2007). Herbicidal effects on non-target vegetation: investigating the limitations of current pesticide registration guidelines. Environmental Toxicology and Chemistry, 26(12), 2634-2643.
Zhang Zhi-Yong; Shan Wei-Li; Song Wen-Cheng (2011). Phytotoxicity and uptake of chlorpyrifos in cabbage. Environmental Chemistry Letters, 9 (4), 547-552.
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