Pectobacterium carotovorum subsp. carotovorum prouzrokovač vlažne truleži brokolija u Srbiji

  • Katarina Gašić Institut za zaštitu bilja i životnu sredinu Odsek za bolesti bilja Teodora Drajzera 9, 11040 Beograd
  • Veljko Gavrilović Institut za zaštitu bilja i životnu sredinu, Teodora Drajzera 9, 11040 Beograd
  • Nenad Dolovac Institut za zaštitu bilja i životnu sredinu, Teodora Drajzera 9, 11040 Beograd
  • Nenad Trkulja Institut za zaštitu bilja i životnu sredinu, Teodora Drajzera 9, 11040 Beograd
  • Svetlana Živković Institut za zaštitu bilja i životnu sredinu, Teodora Drajzera 9, 11040 Beograd
  • Danijela Ristić Institut za zaštitu bilja i životnu sredinu, Teodora Drajzera 9, 11040 Beograd
  • Aleksa Obradović Univerzitet u Beogradu, Poljoprivredni fakultet, Nemanjina 6
DOI: https://doi.org/10.2298/pif.v29i4.7023
Ključne reči: Broccoli||, ||brokoli, Pectobacterium carotovorum subsp. carotovorum||, ||Pectobacterium carotovorum subsp. carotovorum, soft rot||, ||vlažna trulež, 16S rRNA gene||, ||16S rRNA gen, ITS-PCR-RFLP||, ||ITS-PCR-RFLP,

Sažetak


Simptomi vlažne truleži brokolija uočeni su u nekoliko komercijalnih zasada u zapadnoj
Srbiji. Sojevi bakterija izolovani su iz obolelog biljnog tkiva i identifikovani kao Pectobacterium carotovorum subsp. carotovorum korišćenjem konvencionalnih bakterioloških i novijih molekularnih metoda. Svi proučavani sojevi bili su nefluorescentni, Gram-negativni, fakultativno-anaerobni, oksidaza i lecitinaza negativni, katalaza pozitivni, prouzrokujući vlažnu trulež cvetnih drški brokolija, kriški krompira i kriški mrkve, ali nisu indukovali hipersenzitivnu reakciju duvana. Svi sojevi razvijaju se pri 37ºC i u prisustvu 5% NaCl, ne proizvode kiselinu iz α-metil glukozida, sorbitola i maloze, niti redukujuće supstance iz saharoze, ali razlažu laktozu i trehalozu, i ne stvaraju indol. Proučavani sojevi ispoljili su karakterističan razvoj na Loganovoj diferencijalnoj podlozi, i nisu proizvodili plavi pigment na GYCA podlozi niti “fried egg” kolonije na PDA podlozi. Identifikacija sojeva potvrđena je korišćenjem ITS-PCR i ITS-RFLP, kao i analizom sekvenci 16S rRNA gena. Prilikom izvođenja testa patogenosti, svi sojevi prouzrokovali su obezbojavanje tkiva i vlažnu trulež cvetnih drški brokolija.

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Objavljeno
2015/02/10
Kako citirati
Gašić, K., Gavrilović, V., Dolovac, N., Trkulja, N., Živković, S., Ristić, D., & Obradović, A. (2015). Pectobacterium carotovorum subsp. carotovorum prouzrokovač vlažne truleži brokolija u Srbiji. Pesticides and Phytomedicine / Pesticidi I Fitomedicina, 29(4). https://doi.org/10.2298/pif.v29i4.7023
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Vol. 29 Br. 4 (2014): Pesticidi i fitomedicina
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