In vitro i in vivo efekti Pseudomonas spp. i Bacillus sp. na Fusarium acuminatum, Botrytis cinerea i Aspergillus niger na krastavcu

  • Jasmina Zdravković Institut za povrtarstvo, Karadjordjeva 71, 11420 Smederevska Palanka
  • Milan Ugrinovic Institut za povrtarstvo, Karadjordjeva 71, 11420 Smederevska Palanka
  • Milan Zdravkovic Institut za povrtarstvo, Karadjordjeva 71, 11420 Smederevska Palanka
  • Slaviša Djordjević Univerzitet u Beogradu, Poljoprivredni fakultet, Nemanjina 8, 11080 Zemun
  • Snezana Pavlovic -
  • Dragana Jošić Institut za zemljište, Teodora Drajzera 7, Beograd
DOI: https://doi.org/10.2298/pif.v30i3.8070
Ključne reči: Rhizobacteria||, ||Rizobakterije, Pseudomonas spp.||, ||Pseudomonas spp., Bacillus sp.||, ||Bacillus sp., Plant growth||, ||Rast biljaka, Plant diseases||, ||Bolesti biljaka, Cucumbers||, ||Krastavac,

Sažetak


Krastavac (Cucumis sativus L) je važan predstavnik familije Cucurbitaceae, a proizvodnja zdravog rasada je nephodna za visoku produktivnost u plastenicima i na otvorenom polju. Da bi se umanjila primena pesticida i mineralnih đubriva i pri tome sačuvalo zemljište, ispitan je uticaj bakterija stimulatora biljnog rasta (PGPB) na rast biljaka i zaštitu od patogena. Primenjeni su sojevi Pseudomonas spp. sa različitim antimikoznim delovanjem i Bacillus sp. soj Q10 sa PGP aktivnošću. Praćena je antagonistička aktivnost Pseudomonas spp. na fitopatogene gljive izolovane sa biljaka krastavca: F. acuminatum, B. cinerea i A. niger. Testiran je uticaj prekonoćne kulture bakterija, supernatanta i termostabilnih antifungalnih faktora na rast ovih fitopatogena. Pseudomonas sp. K35 i K24 sojevi, koji pokazuju 70-80% inhibicije rasta gljiva bez obzira na primenjenu kulturu ili frakciju, efektivniji su od P.  chlororaphis Q16 i Pseudomonas sp. K27. Sojevi  Pseudomonas spp., koji su ispoljili visok stepen antagonizma, kombinovani su sa sojem Bacillus sp. Q10 i procenjen je uticaj na rast i zdravstveno stanje biljaka krastavca. U zavisnosti od primenjenog soja Pseudomonas sp., razlikovala se dinamika rasta krastavca. Tretman M3 - kombinacija Bacillus sp. Q10 i P. chlororaphis Q16 uticala je na početnu fazu porasta biljaka, dok je treatman M4 - kombinacija Bacillus sp. Q10 i Pseudomonas sp. K24, imala najviše uticaja na visinu biljke na kraju merenja. Uočene su značajne razlike za masu lista i masu biljke (M4) i masu lista (M5 sa sojem K35), dok sadržaj hlorofila i nivo NBI nisu imali značajne razlike kod svih ispitivanih kombinacija. Dobijeni rezultati ukazuju da je M3 pogodan za rane faze razvoja biljke, a kombinacije M4 i M5 pogodne su za zaštitu biljaka i u kasnijim fazama porasta biljaka. Potpuna ekspresija PGP svojstava za M4 i M5 može se utvrditi tek posle testiranja na siromašnim supstratima, koji će biti upoređeni sa rezultaima dobijenim na supstratima sa dovoljno hranljivih materija.

Biografije autora

Jasmina Zdravković, Institut za povrtarstvo, Karadjordjeva 71, 11420 Smederevska Palanka
Principal Research Fellow
Dragana Jošić, Institut za zemljište, Teodora Drajzera 7, Beograd
Principal Research Fellow

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Objavljeno
2015/12/08
Kako citirati
Zdravković, J., Ugrinovic, M., Zdravkovic, M., Djordjević, S., Pavlovic, S., & Jošić, D. (2015). In vitro i in vivo efekti Pseudomonas spp. i Bacillus sp. na Fusarium acuminatum, Botrytis cinerea i Aspergillus niger na krastavcu. Pesticides and Phytomedicine / Pesticidi I Fitomedicina, 30(3). https://doi.org/10.2298/pif.v30i3.8070
Broj časopisa
Vol. 30 Br. 3 (2015): Pesticidi i fitomedicina
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