Određivanje linurona u kamilici LC-MS/MS tehnikom i QuEChERS metodom ekstrakcije

  • Bojana D. Špirović Trifunović Univerzitet u Beogradu, Poljoprivredni fakultet, Nemanjina 6, 11080 Beograd
  • Gorica Lj. Vuković Institut za javno zdravlje, Bulevar despota Stefana 54a, 11000 Beograd
  • Katarina D. Jovanović–Radovanov Univerzitet u Beogradu, Poljoprivredni fakultet, Nemanjina 6, 11080 Beograd
  • Vojislava P. Bursić Univerzitet u Novom Sadu, Poljoprivredni fakultet, Trg Dositeja Obradovića 8, 21000 Novi Sad
  • Maja U. Meseldžija Univerzitet u Novom Sadu, Poljoprivredni fakultet, Trg Dositeja Obradovića 8, 21000 Novi Sad
DOI: https://doi.org/10.2298/pif.v30i2.8198
Ključne reči: Linuron||, ||Linuron, Residues||, ||Ostaci, QuEChERS||, ||QuEChERS, LC-MS/MS||, ||LC-MS/MS, Chamomile||, ||Kamilica,

Sažetak


Linuron je selektivni herbicid, koji se koristi za suzbijanje širokolisnih korova. Njegov
mehanizam delovanja je inhibicija fotosinteze (fotosistema II). Za ekstrakciju ostataka linurona iz uzoraka kamilice korišćena je QuEChERS metoda. Određivanje nivoa ostataka linurona vršeno je tečnom hromatografijom sa masenim spektroskopijom. Linearnost metode je ispitivana u opsegu koncentracija od 0.025 – 0.50 μg/ml, korišćenjem metode kalibracije u matriksu, pri čemu je koeficijent određivanja (R2) bio veći od 0.99. Tačnost metode je ispitivana obogaćivanjem kontrolnih uzoraka kamilice na tri koncentraciona nivoa. Prinos ekstrakcije je bio preko 90 %. Interni standard korišćen za analizu je bio izoproturon-D6. U cvetu kamilice nisu nađeni ostaci linurona, dok su u uzorcima drške bili u opsegu od 0.010-0.040 mg/kg.

Biografija autora

Bojana D. Špirović Trifunović, Univerzitet u Beogradu, Poljoprivredni fakultet, Nemanjina 6, 11080 Beograd
Institut za fitomedicinu, Katedra za pesticide, stručni saradnik

Reference

Anastassiades M., Lehotay S. J., Štajnbaher D., & Schenck F.J. (2003). Fast and easy multiresidue method employing acetonitrile extraction/partitioning and “dispersive solidphase extraction” for the determination of pesticide residues in produce. Journal of AOAC International, 86(2), 412-431.

Baša, Č.H., & Gregorčič A. (2006). Validation of the method for the determination of dithiocarbamates and thiuram disulphide on apple, lettuce, potato, strawberry and tomato matrix. Acta Chimica Slovenica, 53, 100-104.

Baša, Č.H., Velikonja B.Š., & Gregorčič A. (2009). Pesticide Residues in Agricultural Products of the Slovene Origin Found in 2007. Acta Chimica Slovica, 56, 484-493.

Bolofo R.N., & Johnson C.T., (1988). The identification of “Isicakathi” and its medicinal use in Transkei. Bothalia, 18(1), 125-130.

Commission Regulation (EU) No 212/2013 of 11 March 2013 replacing Annex I to Regulation (EC) No 396/2005 of the European Parliament and of the Council as regards additions and modifications with respect to the products covered by that Annex (2013). Official Journal of the European Union, L 68/30-52. Retrieved from https://www.fsai.ie/uploadedFiles/Legislation/Food_Legisation_Links/Pesticides_Residues_in_food/Reg212_2013.pdf (Accession date April 29, 2015).

Council Directive 96/23/EC concerning the performance of analytical methods and the interpretation of results (2002). Official Journal of the European Communities SANCO, Commission decision 2002/657/EC. Retrieved from http://ec.europa.eu/food/food/chemicalsafety/residues/workdoc_2002_en.pdf (Accession date April29, 2015.

Dreassi E., Zanfini A., Zizzari A.T., La Rosa C., Botta M., & Corbini G. (2010). Lc/Esi/Ms/Ms determination of postharvest fungicide residues in citrus juices, LWTFood Science and Technology. 43(9), 1301-1306.

Ebato M., & Yonebayashi K. (2005). Method for estimating competitive adsorption of herbicides on soils. Journal of Pesticide Science 30(3), 220-224.

Hutchings A. (1989). A survey and analysis of traditional medicinal plants as used by the Zulu, Xhosa and Sotho. Bothalia, 19(1), 112-123.

Jovanović-Radovanov K., Radojević R., & Petrović D. (2012). Weed control methods in chamomile production in Serbia. In Proceedings of the Seventh Conference on Medicinal and Aromatic Plants of Southeast European Countries (435-441). Retrieved from http://www.amapseec.org/Proceedings%20of%20VII%20CMAPSEEC.pdf

Lozano A., Rajski L., Belmonte-Valles N., Uclés A., Uclés S., Mezcua M., & Fernández-Alba A. R. (2012). Pesticide analysis in teas and chamomile by liquid chromatography and gas chromatography tandem mass spectrometry using a modified QuEChERS method: Validation and pilot survey in real samples. Journal of Chromatography A. 1268, 109-122.

Momčilović B., Ivičić N., Bošnjak I., Stanić G., Ostojić Z., & Hrlec G. (1999). “Više nije bolje” - Prilog procjeni toksikološkog rizika olova i kadmija te herbicida linuron, fluazifop-p-butyl i cycloxydim u osušenom cvijetu kamilice (Chamomilla recutita L. Rauschert). Arhiv za higijenu rada i toksikologiju/Archives of Industrial Hygiene and Toxicology 50(2), 201-210.

Pizzutti I.R., de Kok A., Zanella R., Adaime M.B., Hiemstra M., Wickert C., & Prestes O.D. (2007). Method validation for the analysis of 169 pesticides in soya grain, without clean up, by liquid chromatography-tandem mass spectrometry using positive and negative electrospray ionization. Journal of Chromatography A., 1142(2), 123-136.

Pravilnik o maksimalno dozvoljenim količinama ostataka sredstava za zaštitu bilja u hrani i hrani za životinje i o hrani za životinje za koju se utvrđuju maksimalno dozvoljene količine ostataka sredstava za zaštitu bilja (2014). Službeni glasnik Repulike Srbije/Official Gazette of RS, 29, 2014. Retrieved from http://www.uzb.minpolj.gov.rs/index.php?option=com_content&view=article&id=147%3A2010-11-28-14-28-09&Itemid=12&lang=sr (Accession date April, 29, 2015).

Regulation (EC) No 396/2005 of the European Parliament and of the Council of 23 February 2005 on maximum residue levels of pesticides in or on food and feed of plant and animal origin and amending Council Directive 91/414/EEC (2005). Official Journal of the European Union, L 70. Retrieved from http://eur-lex.europa.eu/legal-content/EN/ALL/?uri=CELEX:32005R0396 (Accession date April 29. April, 2015).

Singh O., Khanam Z., Misra N., & Srivastava M.K. (2011). Chamomile (Matricaria chamomilla L.): An overview. Pharmacognosy Review, 5(9), 82-95. doi:10.4103/0973-7847.79103, pmid:22096322

Soler C., & Picó Y. (2007). Recent trends in liquid chromatography-tandem mass spectrometry to determine pesticides and their metabolites in food. Trends Analitical

Chemistry 26(2), 103-115.

Stevanović D., Vrbničanin S., Jevđović R. (2007). Weeding of cultivated chamomile in Serbia (Proceedings of I International Symposium on Chamomile Research, Development and Production). Acta Horticulturae, 749, 149-155.

Vieira R.F., Bizzo H.R., & Deschamps C.(2010). Genetic resources of aromatic plants from Brazil. Israel Journal of Plant Science, 58(3-4), 263-271.

Vuković G., Bursić V., Lazić S., & Špirović B. (2012a). Matrix effect of apple, cherry and peach on pesticide residues analysis. In Proceedings of a Golden Jubilee Meeting of the Serbian Chemical Society (CD) (pp 12-15). Belgrade: SCS.

Vuković G., Shtereva D., Bursić V., Mladenova R., & Lazić S. (2012b). Application of GC–MSD and LC–MS/MS for the determination of priority pesticides in baby foods in Serbian market. LWT–Food Science and Technology, 49, 312-319.

Objavljeno
2015/07/30
Kako citirati
Špirović Trifunović, B. D., Vuković, G. L., Jovanović–Radovanov, K. D., Bursić, V. P., & Meseldžija, M. U. (2015). Određivanje linurona u kamilici LC-MS/MS tehnikom i QuEChERS metodom ekstrakcije. Pesticides and Phytomedicine / Pesticidi I Fitomedicina, 30(2). https://doi.org/10.2298/pif.v30i2.8198
Broj časopisa
Vol. 30 Br. 2 (2015): Pesticidi i fitomedicina
Rubrika
Originalni naučni članak
Autori koji objavljuju u ovom časopisu pristaju na sledeće uslove:

  1. Autori zadržavaju autorska prava i pružaju časopisu pravo prvog objavljivanja rada i licenciraju ga "Creative Commons Attribution licencom" koja omogućava drugima da dele rad, uz uslov navođenja autorstva i izvornog objavljivanja u ovom časopisu.

  2. Autori mogu izraditi zasebne, ugovorne aranžmane za neekskluzivnu distribuciju članka objavljenog u časopisu (npr. postavljanje u institucionalni repozitorijum ili objavljivanje u knjizi), uz navođenje da je članak izvorno objavljen u ovom časopisu.

  3. Autorima je dozvoljeno i podstiču se da postave objavljeni članak onlajn (npr. u institucionalni repozitorijum ili na svoju internet stranicu) pre ili tokom postupka prijave rukopisa, s obzirom da takav postupak može voditi produktivnoj razmeni ideja i ranijoj i većoj citiranosti objavljenog članka (Vidi Efekti otvorenog pristupa).