Uticaj rizobakterija i useva na klijanje i rani porast viline kosice (Cuscuta campestris Yunk.)
Sažetak
Za ispitivanje uticaja rizobakterija (Planth Growth Promoting Rhizobacteria - PGPR) na
klijanje semena i stope klijanja viline kosice (Cuscuta campestris Yunk.), korišćene su bakterijske kulture: Bacillus licheniformis (MO1), B. pumilus (MO2) i B. amyloliquefaciens (MO3) izolovane iz stajnjaka; B. megatherium ZP6 (MO4) iz rizosfere kukuruza; Azotobacter chroococcum Ps1 (MO5) i Pseudomonas fluorescens (MO6). Svakodnevno u periodu od deset dana brojana su proklijala semena. Takođe, za praćenje uticaja semena biljaka domaćina na klijanje semena i početni porast klijanaca viline kosice u mraku i pri tretmanu belom svetlošću korišćena su semena četiri biljke domaćina (lubenica, crvena detelina, lucerka i šećerna repa). Proklijala semena su prebrojavana svakodnevno u periodu od deset dana, a poslednjeg dana su izmerene dužine klijanaca.
Dobijeni rezultati ukazuju da su tretmani MO3, MO4 i MO6 ispoljili inhibitorni (15%, 65%
i 52%), a tretmani MO1 (Bacillus licheniformis), MO2 (B. amyloliquefaciens) i MO5 (Azotobacter chroococcum Ps1) stimulativni efekat (3%, 3% i 19%) na klijanje semena viline kosice. Takođe, rezultati dobijeni sa semenima biljaka domaćina ukazuju da je za podsticanje klijanja semena viline kosice, osim prisustva domaćina (73-79%, kontrola 80%), kao inicijalni faktor značajna i svetlost (83-95%, kontrola 95%).
Reference
Ahmad, F., Ahmad, I., & Khan, M.S. (2008). Screening of free-living rhizospheric bacteria for their multiple plant growth promoting activities. Microbiological Research, 163(2), 173-181.
Benvenuti, S., Pompeiano, A., Macchia, M., & Miele, S. (2002). Orobanche seed bank dynamics in tobacco by using a germination stimulant. In 12th European Weed Research Society Symposium, Wageningen (pp 380-381). Dordrecht, The Netherlands: Academic Publishers.
Bhat, J.M., & Alagawadi, A.R. (1998). Seed borne nature of Azotobacter chroococcum in chilli (Capsicum annum) and its role in seed germination and plant growth. Hindustan Antibiotic Bulletin, 40, 20-30.
Carrillo-Castaneda, G., Juarez Munos, J., Peralta-Videa, J.R., Gomez, E., Tiemannb, K.J., Duarte-Gardea, M., & Gardea-Torresdey, J.L. (2002). Alfalfa growth promotion by bacteria grown under iron limiting conditions. Advances in Environmental Research, 6(3), 391-399.
Egamberdiyeva, D. (2007). The effect of plant growth promoting bacteria on growth and nutrient uptake of maize in two different soils. Applied Soil Ecology, 36(2-3), 184-189.
Funk, H.T., Berg, S., Krupinska, K., Maier, U.G., & Krause, K. (2007). Complete DNA sequences of the plastid genomes of two parasitic flowering plant species, Cuscuta reflexa and Cuscuta gronovii. BMC Plant Biology 7, 45. doi 10.1186/1471-2229-7-45.
Garcia, M.A., Costea, M., Kuzmina, M., & Stefanović, S. (2014). Phylogeny, character evolution, and biogeography of Cuscuta (dodders; Convolvulaceae) inferred from coding plastid and nuclear sequences. American Journal of Botany, 101(4), 670–690. pmid:24688058
Gutierrez-Manero, F.J., Ramos-Solano, B., Probanza, A., Mehouachi, J., Tadeo, F.R., & Talon, M. (2001). The plant-growth-promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiolically active gibberellins. Physiologia Plantarium, 111(2), 206-211. doi:10.1034/j.1399-3054.2001.1110211.x
Haidar, M.A. (2003). Characterization of the interaction between cryptochromes and phytochromes in blue light-induced coiling and prehaustoria development of dodder (Cuscuta campestris) seedlings. Annals of Applied Biology, 143(1), 57-62.
Haidar, M.A., Orr, G.L., & Westra, P. (1997). Effects of light and mechanical stimulation on coiling and prehaustoria formation in Cuscuta spp. Weed Research, 37(4), 219-228. doi:10.1046/j.1365-3180.1997.d01-36.x
Kloepper, J.W., Lifshitz, R., & Zablotowicz, R.M. (1989). Freeliving bacterial inocula for enhancing crop productivity. Trends in Biotechnology, 7(2), 39-43.
Maguire, J. (1962). Speed of germination aid in selection and evaluation for seedling emergence and vigour. Crop Science, 2, 176-177.
Martinez-Toledo, M.V., Gonzalez-Lopez, J., de la Rubia T., Moreno, J., & Ramos-Cormenzana, A. (1988). Effect of inoculation with Azotobacter chroococcum on nitrogenase activity of Zea mays roots grown in agricultural soils under aseptic and non-sterile conditions. Biology and Fertility of Soils, 6(2), 170-173.
McNeal, J.R., Arumugunathan, K., Kuehl, J.V., Boore, J.L., & de Pamphilis, C.W. (2007a). Systematics and plastid genome evolution of the cryptically photosynthetic parasitic plant genus Cuscuta (Convolvulaceae). BMC Biology 5, 55. doi 10.1186/1741-7007-5-55.
McNeal, J.R., Kuehl, J.V., Boore, J.L., & de Pamphilis, C.W. (2007b). Complete plastid genome sequences suggest strong selection for retention of photosynthetic genes in the parasitic plant genus Cuscuta. BMC Plant Biology 7, 57. doi 10.1186/1471-2229-7-57.
Miche, L., Bouillant, M.L., Rohr, R., Salle, G., & Bally, R. (2000). Physiological and cytological studies on the inhibition of Striga seed germination by the plant growth-promoting bacterium Azospirillum brasilense. European Journal of Plant Pathology, 106(4), 347-351.
Orr, G.L., Haidar, M.A., & Orr, D.A. (1996). Small seed dodder (Cuscuta planiflora) phototropism toward farred when in white light. Weed Science, 44(2), 233-240.
Ping, L., & Boland, W. (2004). Signals from the underground: bacterial volatiles promote growth in Arabidopsis. Trends in Plant Science, 9(6), 263-266.
Rodriguez, H., & Fraga, R. (1999). Phosphate solubilizing bacteria and their role in plant growth promotion. Biotechnology Advances, 17(4-5), 319-339.
Rueda-Puente, E.O., Garcia-Hernandez, J.L., Preciado-Rangel, P., Murillo-Amador, B., Tarazon-Herrera, M.A., Flores-Hernandez, A. … Troyo-Dieguez, E. (2007). Germination of Salicornia bigelovii ecotypes under stressing conditions of temperature and salinity and ameliorative effects of plants growth-promoting bacteria. Journal of Agronomy and Crop Science, 193(3), 167-176.
Runyon, J.B., Mescher, M.C., & Moraes, C.D. (2006). Volatile chemical cues guide host location and host selection by parasitic plants. Science, 313, 1964-1967.
Ryu, C.M., Farag, M.A., Hu, C.H., Reddy, M.S., Wei, H.X., Pare, P.W., & Kloepper, J.W. (2003). Bacterial volatiles promote growth in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 100(8), 4927-4932.
Saric, M., Vrbnicanin, S., Bozic, D., & Raicevic, V. (2009). Effect of plant growth-promoting rhizobacteria on the germination of Cuscuta campestris Yunck. In Proceedings of 10th World Congress of Parasitic Plants, Kusadasi (p 73). Kusadasi, Turkey: IPPS and Ege University.
Shishido, M., Massicotte, H.B., & Chanway, C.P. (1996). Effect of plant growth promoting Bacillus strains on pine and spruce seedling growth and mycorrhizal infection. Annals of Botany, 77(5), 433-441.
Sturz, A.V., & Nowak, J. (2000). Endophytic communities of rhizobacteria and the strategies required to create yield enhancing associations with crops. Applied Soil Ecology, 15(2), 183-190.
Tada, Y., Sugai, M., & Furuhashi, K. (1996). Haustoria of Cuscuta japonica, a holoparasitic flowering plant, are induced by the cooperative effects of far-red light and tactile stimuli. Plant Cell Physiology, 37(8), 1049-1053.
Vail, S.L., Dailey, O.D., Blanchard, E.J., Pepperman, A.B., & Riopel, J.L. (1990). Terpenoid precursors of strigol as a seed germination stimulant of broomrape (Orobanche ramosa) and witchweed (Striga asiatica). Journal of Plant Growth Regulation 9, 77-83.
Vrbničanin, S., Božić, D., Sarić, M., Pavlović, D., & Raičević, V. (2011): Effect of plant growth promoting rhizobacteria on Ambrosia artemisiifolia L. seed germination. Pesticidi i fitomedicina/Pesticides and Phytomedicine, 26(2), 141-146.
Vrbničanin, S., Jovanović, Lj., Božić, D., Raičević, V., & Pavlović, D. (2008a). Germination of Iva xanthifolia, Amaranthus retroflexus and Sorghum halepense under media with microorganisms. Journal of Plant Diseases and Protection, 21 (Special issue), 297-302.
Vrbničanin, S., Jovanović, Lj., Božić, D., Pavlović, D., & Raičević, V. (2008b). Effect of growth-promoting bacteria on germination of Datura stramonium L., Abutilon theophrasti Medik., Onopordon acanthium L. and Verbascum thapsus L. In Book of abstracts, 5th International Weed Science Congress, Vancouver, Canada, 127.
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