Karakterizacija izolata Pectobacterium carotovorum subsp. carotovorum sa kupusa izolovanih tokom skorije pojave u Bosni i Hercegovini
Sažetak
U radu je identifikovan i okarakterisan prouzrokovač vlažne truleži kupusa u Semberiji.
Simptomi su se javljali na listu u vidu lezija vlažnog izgleda uz prisustvo specifičnog mirisa.
Bolest je zahvatila 20 do 30% useva. Izolacija patogena prouzrokovača bolesti je vršena na
hranljivom agaru (NA), King’s B i Kristal violet pektatnoj podlozi (CVP). Za dalja istraživanja
odabrano je osam krem-beličastih, okruglih i ispupčenih bakterijskih kolonija koje su stvarale
karakteristična udubljenja na CVP podlozi. Svi izolati su bili gram negativni, fakultativni anaerobi,
oksidaza negativni, katalaza pozitivni, nefluorescentni na King-ovoj podlozi B, negativni na
stvaranje levana i arginin dihidrolaze. Svi izolati su prouzrokovali vlažnu trulež na kupusu i
kriškama krompira 24 časa nakon inokulacije u uslovima visoke relativne vlažnosti. Reakcija
lančane reakcije polimeraze (PCR) je vršena u cilju preliminarne identifikacije izolata, primenom
tri para specifičnih prajmera: F0145/E2477 (specifični za Pectobacterium carotovorum subsp.
carotovorum), Br1f/L1r (specifični za P. carotovorum subsp. brasiliensis) i ECA1f/ECA2r (specifični
za P. atrosepticum). Kod svih izolata amplifikovani su produkti veličine 666 baznih parova
korišćenjem F0145/E2477 para prajmera, što je ukazalo na prisustvo bakterije P. carotovorum
subsp. carotovorum. Dalja genetička karakterizacija je vršena na osnovu analize sekvenci
konzervisanih gena gapA i mdh. BLAST analiza je potvrdila identifikaciju izolata na osnovu
homologije sa sojevima P. carotovorum subsp. carotovorum deponovanim u NCBI bazi i to
od 99.39% (Q. cover 100%, E. value 0.0) sa sojem M34 (Acc.no. KY047594) za sekvence gapA
gena i 100% (Q. cover 100%, E. value 0.0) sa Pcc t0437 (Acc.no. KC337296) za sekvence mdh
gena. Filogenetska analiza je pokazala genetičku homogenost izolata poreklom sa kupusa,
svrstavajući ih u jedan klaster.
Reference
Agrios, G.N. (2006). Bacterial soft rots (5th ed). San Diego, USA: Academic Press.
Aksoy, H.M., Ozturk, M., & Aktas, A. (2017). First report of Pectobacterium carotovorum subsp. carotovorum causing soft rot on white head cabbage in Turkey. Journal of Plant Pathology, 99(3). doi: http://dx.doi.org/10.4454/jpp.v99i3.3936
Anonimous, (1990). Bacterial soft rot of vegetables, fruits, and ornamentals. Report on plant disease (943). University of Illinois Extension. https://ipm.illinois.edu/diseases/rpds/943.pdf
Arsenijević, M., & Obradović, A. (1996). Occurrence of bacterial wilt and soft rot of seed cabbage plants (Brassica oleracea var. capitata L.) in Yugoslavia. Journal of Phytopathology, 144(6), 315-319. doi: https://doi.org/10.1111/j.1439-0434.1996.tb01535.x
Ausubel, F.M., Brent, R., Kingston, R.E., Moore, D.D., Seidman, J.G., Smith, J.A., & Struhl, K. (2003). Current protocols in molecular biology (vol. 1). New York, USA: John Wiley & Sons.
Avrova, A.O., Hyman, L.J., Toth, R.L., & Toth, I.K. (2002). Application of amplified fragment length polymorphism fingerprinting for taxonomy and identification of the soft rot bacteria Erwinia carotovora and Erwinia chrysanthemi. Applied and Environmental Microbiology, 68(4), 1499-1508. doi: 10.1128/aem.68.4.1499-1508.2002
Baghaee-Ravari, S., Rahimian, H., Shams-Bakhsh, M., Lopez-Solanilla, E., Antunez-Lamas, M., & Rodriguez-Palenzuela, P. (2011). Characterization of Pectobacterium species from Iran using biochemical and molecular methods. European Journal of Plant Pathology, 129(3), 413-425.
Bewick, T.A. (1994). Cabbage: uses and production. Fact Sheet (HS-712). University of Florida, Florida Cooperative Extension Service. https://www.oocities.org/habbage/cab.pdf
Bhat, K.A., Bhat, N.A., Masoodi, S.D., Mir, S.A., Zargar, M.Y., & Sheikh, P.A. (2010). Studies on status and host range of soft rot disease of cabbage (Brassica oleracea var capitata) in Kashmir Valley. Journal of Phytology, 2(10), 55-59.
Bhat, K.A., Bhat, N.A., Mohiddin, F.A., Mir, S.A., & Mir, M.R. (2012). Management of post-harvest Pectobacterium soft rot of cabbage (Brassica oleracea var capitata L.) by biocides and packing material. African Journal of Agricultural Research, 7(28), 4066-4074. doi: 7. 4066-4074. 10.5897/AJAR12.1197
Chiang, M.S., Chong, C., Landry, B.S., & Crete, R. (1993). Cabbage: Brassica oleracea subsp. capitata L. In G. Kalloo & B.O. Bergh (eds.), Genetic improvement of vegetable crops (pp 113-155). New York, USA: Pergamon Press.
Cigna, J., Dewaegeneire, P., Beury, A., Gobert, V., & Faure, D. (2017). A gapA PCR-sequencing assay for identifying the Dickeya and Pectobacterium potato pathogens. Plant
Disease, 101(7), 1278-1282. doi: https://doi.org/10.1094/PDIS-12-16-1810-RE
Czajkowski, R., Perombelon, M.C.M., Jafra, S., Lojkowska, E., Potrykus, M., Van Der Wolf, J.M., & Sledz, W. (2015). Detection, identification and differentiation of Pectobacterium and Dickeya species causing potato blackleg and tuber soft rot: a review. Annals of Applied Biology, 166(1), 18-38. doi: 10.1111/aab.12166
De Boer, S.H., & Ward, L.J. (1995). PCR detection of Erwinia carotovora subsp. atroseptica associated with potato tissue. Phytopathology, 85(8), 854-858. doi: 10.1094/Phyto-85-854
Dees, M.W., Lysoe, E., Rossmann, S., Perminow, J., & Brurberg, M.B. (2017). Pectobacterium polaris sp. nov., isolated from potato (Solanum tuberosum). International Journal of Systematic and Evolutionary Microbiology, 67(12), 5222-5229. doi: 10.1099/ijsem.0.002448
Duarte, V., De Boer, S.H., Ward, L.J., & De Oliveira, A.M. (2004). Characterization of atypical Erwinia carotovora strains causing blackleg of potato in Brazil. Journal of Applied Microbiology, 96(3), 535-545. doi: 10.1111/j.1365-2672.2004.02173.x
Gardan, L., Gouy, C., Christen, R., & Samson, R. (2003). Elevation of three subspecies of Pectobacterium carotovorum to species level: Pectobacterium atrosepticum sp. nov., Pectobacterium betavasculorum sp. nov. and Pectobacterium wasabiae sp. nov. International Journal of Systematic and Evolutionary Microbiology, 53(2), 381-391. doi: 10.1099/ijs.0.02423-0
Kettani-Halabi, M., Terta, M., Amdan, M., El Fahime, M., Bouteau, F., & Ennaji, M.M. (2013). An easy, simple inexpensive test for the specific detection of Pectobacterium carotovorum subsp. carotovorum based on sequence analysis of the pmrA gene. BMC Microbiology, 13(1), 176. doi: 10.1186/1471-2180-13-176
Khayi, S., Cigna, J., Chong, T.M., Quetu-Laurent, A., Chan, K.G., Helias, V., & Faure, D. (2016). Transfer of the potato plant isolates of Pectobacterium wasabiae to Pectobacterium parmentieri sp. nov. International Journal of Systematic and Evolutionary Microbiology, 66(12),
-5383. doi: 10.1099/ijsem.0.001524
Khayi, S., Des Essarts, Y.R., Quetu-Laurent, A., Moumni, M., Helias, V., & Faure, D. (2015). Genomic overview of the phytopathogen Pectobacterium wasabiae strain RNS 08.42. 1A suggests horizontal acquisition of quorumsensing genes. Genetica, 143(2), 241-252. doi: 10.1007/s10709-014-9793-2
Li, X., Ma, Y., Liang, S., Tian, Y., Yin, S., Xie, S., & Xie, H. (2018). Comparative genomics of 84 Pectobacterium genomes reveals the variations related to a pathogenic lifestyle. BMC Genomics, 19(1), 889. doi: 10.1186/s12864-018-5269-6
Li, L., Yuan, L., Shi, Y., Xie, X., Chai, A., Wang, Q., & Li, B. (2019). Comparative genomic analysis of Pectobacterium carotovorum subsp. brasiliense SX309 provides novel insights into its genetic and phenotypic features. BMC Genomics, 20(1), 486. doi: 10.1186/s12864-019-5831-x
Ma, B., Hibbing, M.E., Kim, H.S., Reedy, R.M., Yedidia, I., Breuer, J. … Charkowski, A.O. (2007). Host range and molecular phylogenies of the soft rot enterobacterial genera Pectobacterium and Dickeya. Phytopathology, 97(9), 1150-1163. doi: 10.1094/PHYTO-97-9-1150
Mauzey, S.J., Koike, S.T., & Bull, C.T. (2011). First report of bacterial blight of cabbage (Brassica oleracea var. capitata) caused by Pseudomonas cannabina pv. alisalensis in California. Plant Disease, 95(1), 71. doi: 10.1094/PDIS-09-10-0642
Mitrović, P. (1997). Cabbage pathogens. Novi Sad, Yugoslavia: Faculty of Agriculture. Retrieved from: http://agris.fao.org/agris-search/search.do?recordID=YU19970133030
Mansfield, J., Genin, S., Magori, S., Citovsky, V., Sriariyanum, M., Ronald, P., … Foster, G.D. (2012). Top 10 plant pathogenic bacteria in molecular plant pathology. Molecular Plant Pathology, 13(6), 614-629. doi: 10.1111/j.1364-3703.2012.00804.x
Moleleki, L.N., Onkendi, E.M., Mongae, A., & Kubheka, G.C. (2013). Characterisation of Pectobacterium wasabiae causing blackleg and soft rot diseases in South Africa. European Journal of Plant Pathology, 135(2), 279-288. doi: 10.1007/s10658-012-0084-4
Nabhan, S., Wydra, K., Linde, M., & Debener, T. (2012a). The use of two complementary DNA assays, AFLP and MLSA, for epidemic and phylogenetic studies of pectolytic enterobacterial strains with focus on the heterogeneous species Pectobacterium carotovorum. Plant Pathology, 61(3), 498-508. doi: 10.1111/j.1365-3059.2011.02546.x
Nabhan, S., De Boer, S.H., Maiss, E., & Wydra, K. (2012b). Taxonomic relatedness between Pectobacterium carotovorum subsp. carotovorum, Pectobacterium carotovorum subsp. odoriferum and Pectobacterium carotovorum subsp. brasiliense subsp. nov. Journal of Applied Microbiology, 113(4), 904-913. doi: 10.1111/j.1365-2672.2012.05383.x
Nabhan, S., De Boer, S.H., Maiss, E., & Wydra, K. (2013). Pectobacterium aroidearum sp. nov., a soft rot pathogen with preference for monocotyledonous plants. International Journal of Systematic and Evolutionary Microbiology, 63(7), 2520-2525. doi: 10.1099/ijs.0.046011-0
Nazerian, E., Sijam, K., Mior Ahmad, Z.A., & Vadamalai, G. (2011). First report of cabbage soft rot caused by Pectobacterium carotovorum subsp. carotovorum in Malaysia. Plant Disease, 95(4), 491. doi: 10.1094/PDIS-09-10-0683
Onkendi, E.M,. & Moleleki, L.N. (2014). Characterization of Pectobacterium carotovorum subsp. carotovorum and brasiliense from diseased potatoes in Kenya. European Journal of Plant Pathology, 139(3), 557-566. doi: 10.1007/s10658-014-0411-z
Oskiera, M., Kałużna, M., Kowalska, B., & Smolinska, U. (2017). Pectobacterium carotovorum subsp. Odoriferum on cabbage and Chinese cabbage: identification, characterization and taxonomic relatedness of bacterial soft rot causal agents. Journal of Plant Pathology, 99(1), 149-160. doi: http://dx.doi.org/10.4454/jpp.v99i1.3831
Pasanen, M., Laurila, J., Brader, G., Palva, E.T., Ahola, V., van der Wolf, J... & Pirhonen, M. (2013). Characterisation of Pectobacterium wasabiae and Pectobacterium carotovorum subsp. carotovorum isolates from diseased potato plants in Finland. Annals of Applied Biology, 163(3), 403-19. doi: https://doi.org/10.1111/aab.12076
Perombelon, M.C.M., & Kelman, A. (1980). Ecology of the soft rot Erwinias. Annual Review of Phytopathology, 18, 361-387. doi: https://doi.org/10.1146/annurev.py.18.090180.002045
Popović, T., Jelušić, A., Milovanović, P., Janjatović, S., Budnar, M., Dimkić, I., & Stanković, S. (2017). First report of Pectobacterium atrosepticum, causing bacterial soft rot on calla lily in Serbia. Plant Disease, 101(12), 2145. doi: https://doi.org/10.1094/PDIS-05-17-0708-PDN
Popović, T., Jelušić, A., Dimkić, I., Stanković, S., Poštić, D., Aleksić, G., & Veljović Jovanović, S. (2019a). Molecular characterization of Pseudomonas syringae pv. coriandricola and biochemical changes attributable to the pathological response on its hosts carrot, parsley, and parsnip. Plant Disease, 103(2), 3072-3082. doi: https://doi.org/10.1094/PDIS-03-19-0674-RE
Popović, T., Mitrović, P., Jelušić, A., Dimkić, I., Marjanović-Jeromela, A., Nikolić, I., & Stanković, S. (2019b). Genetic diversity and virulence of Xanthomonas campestris pv. campestris isolates from Brassica napus and six Brassica oleracea crops in Serbia. Plant Pathology, 68(8), 1448-1457. doi: https://doi.org/10.1111/ppa.13064
Rafiei, S., Khodakaramian, G., & Baghaee-Ravari, S. (2015). Characterization of Pectobacterium species isolated from vegetable crops in north-west of Iran. African Journal of
Agricultural Research, 10(46), 4258-4267. doi: https://doi.org/10.5897/AJAR2015.9551
Rajeh, O.N., & Khlaif, H. (2000). Soft rot disease of vegetables in Jordan: host range, reaction of some potato cultivars to the infection and effect of planting date. Dirasat Agricultural Sciences, 27(2), 149-157.
Sarfraz, S., Riaz, K., Oulghazi, S., Cigna, J., Sahi, S.T., Khan, S.H., & Faure, D. (2018). Pectobacterium punjabense sp. nov., isolated from blackleg symptoms of potato plants in Pakistan. International Journal of Systematic and Evolutionary Microbiology, 68(11), 3551-3556. doi: 10.1099/ijsem.0.003029
Schaad, N.W., Jones, J.B., & Chun. W. (eds.) (2001). Laboratory guide for identification of plant pathogenic bacteria (3rd ed.). St. Paul, Minnesota, USA: American Phytopathological Society Press.
Shirshikov, F.V., Korzhenkov, A.A., Miroshnikov, K.K., Kabanova, A.P., Barannik, A.P., Ignatov, A.N., & Miroshnikov, K.A. (2018). Draft genome sequences of new genomospecies “Candidatus Pectobacterium maceratum” strains, which cause soft rot in plants. Genome Announcements, 6(15), e00260-18. doi: 10.1128/genomeA.00260-18
Tambong, J.T. (2019). Taxogenomics and systematics of the genus Pantoea. Frontiers in Microbiology, 10, 2463. doi: https://doi.org/10.3389/fmicb.2019.02463
Tamura, K., Dudley, J., Nei, M., & Kumar, S. (2011). MEGA software (version 5): Molecular evolutionar genetics analysis. Arizona State University: Center for Evolutionary Functional Genomics Biodesign Institute.
Vlajić, S., Maširević, S., Barać, R., Iličić, R., Gvozdanović-Varga, J., & Božić, V. (2017). Bolesti kupusa tokom 2016. godine. In: XXII Savetovanje o biotehnologiji, Zbornik radova 1, 309-314.
Waleron, M., Misztak, A., Waleron, M., Franczuk, M., Jonca, J., Wielgomas, B. … & Waleron, K. (2019). Pectobacterium zantedeschiae sp. nov. a new species of a soft rot pathogen isolated from Calla lily (Zantedeschiae spp.). Systematic and Applied Microbiology, 42(3), 275-283. doi: 10.1016/j.syapm.2018.08.004
Waleron, M., Misztak, A., Waleron, M., Franczuk, M., Wielgomas, B., & Waleron, K. (2018). Transfer of Pectobacterium carotovorum subsp. carotovorum strains isolated from potatoes grown at high altitudes to Pectobacterium peruviense sp. nov. Systematic and Applied Microbiology, 41(2), 85-93. doi: 10.1016/j.syapm.2017.11.005
Watanabe, K.N., & Pehu, E. (1997). Plant biotechnology and plant genetic resources for sustainability and productivity. Texas, USA: Landes Company and Accademic Press.
Yishay, M., Burdman, S., Valverde, A., Luzzatto, T., Ophir, R., & Yedidia, I. (2008). Differential pathogenicity and genetic diversity among Pectobacterium carotovorum ssp. carotovorum isolates from monocot and dicot hosts support early genomic divergence within this taxon. Environmental Microbiology, 10(10), 2746-2759. doi: 10.1111/j.1462-2920.2008.01694.x
Zaczek-Moczydłowska, M.A., Fleming, C.C., Young, G.K., Campbell, K., & O’Hanlon, R. (2019). Pectobacterium and Dickeya species detected in vegetables in Northern Ireland. European Journal of Plant Pathology, 154(3), 635-647. doi: 10.1007/s10658-019-01687-1
Zeigler, D.R. (2003). Gene sequences useful for predicting relatedness of whole genomes in bacteria. International Journal of Systematic and Evolutionary Microbiology, 53(6), 1893-1900. doi: 10.1099/ijs.0.02713-0
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