Therapeutic Efficacy of Plant-Based Hydrogels in Burn Wound Healing: Focus on Satureja Montana L. and Origanum Vulgare L.

Grigory A Demyashkin; Mikhail A Parshenkov; Alibek A Tokov; Tatiana P Sataieva; Ludmila A Shevkoplyas; Bekeev S Said; Gasanbekov N Shamil

doi:10.5937/scriptamed56-56050

Grigory A Demyashkin Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119048, Trubetskaya St., 8/2, Moscow, Russia https://orcid.org/0000-0001-8447-2600
Mikhail A Parshenkov Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University) https://orcid.org/0009-0004-7170-8783
Alibek A Tokov Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119048, Trubetskaya St., 8/2, Moscow, Russia https://orcid.org/0009-0002-2583-9374
Tatiana P Sataieva Department of Microbiology, Virology and Immunology, Crimean Federal University Named after V.I.Vernadsky, Order of the Red Banner of Labor Medical Institute Named after. S. I. Georgievsky, 295006, Lenina Blvd, 5/7, Simferopol, Russia https://orcid.org/0000-0001-6451-7285
Ludmila A Shevkoplyas Department of Microbiology, Virology and Immunology, Crimean Federal University Named after V.I.Vernadsky, Order of the Red Banner of Labor Medical Institute Named after. S. I. Georgievsky, 295006, Lenina Blvd, 5/7, Simferopol, Russia https://orcid.org/0000-0003-1166-4585
Bekeev S Said Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119048, Trubetskaya St., 8/2, Moscow, Russia https://orcid.org/0000-0002-1841-4653
Gasanbekov N Shamil Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119048, Trubetskaya St., 8/2, Moscow, Russia https://orcid.org/0009-0007-3545-3965

DOI: https://doi.org/10.5937/scriptamed56-56050

Keywords: Burns, Satureja, montana L., Origanum, vulgare L., Plant extracts, Inflammation, Regeneration

Abstract

Background/Aim: Burn injuries present a critical issue for healthcare systems worldwide. They often lead to numerous complications, including persistent inflammation, impaired healing and heightened infection risks. The growing challenge of antibiotic resistance further complicates treatment, making bacterial infections harder to control and wound healing less effective. Existing treatment methods are often limited, highlighting the need for alternative approaches. Aim of this study was to analyse influence of hydrolate-based gels derived from Satureja montana L. and Origanum vulgare L. on the healing of burn wounds.

Methods: New Zealand white rabbits (n = 25) with standardised Grade IIIa thermal burns were assigned to treatment groups receiving hydrolate-based gels (Satureja montana L. or Origanum vulgare L.), conventional therapy (Betadine (povidone-iodine) and Levomecol (methyluracil, chloramphenicol)), or no treatment. Inflammatory responses were tracked by collecting blood samples at the study’s begin, on day 3 and at one- and two-weeks post-burn, with subsequent ELISA analysis. Histological evaluation of tissue regeneration and inflammatory response was conducted using haematoxylin and eosin staining on days 3, 7 and 14.

Results: By day 14, Satureja montana L. demonstrated the most effective reduction in interleukin 6 (IL-6), tumour necrosis factor alpha (TNF-α) and C-reactive protein (CRP) levels, nearing baseline values. This biochemical improvement correlated with histological findings, showing advanced epithelial regeneration, reduced inflammatory cell infiltration and clearer dermal-epidermal boundaries. The results highlight Satureja montana L. as the most effective treatment in both inflammation control and tissue repair compared to Origanum vulgare L. and conventional therapy.

Conclusion: Hydrolate-based gels from Satureja montana L. and Origanum vulgare L. represent a promising option for adjunctive burn wound therapy. The Satureja montana L. group demonstrated significant improvements in inflammation control and tissue regeneration, while Origanum vulgare L. showed results comparable to standard therapy.

Author Biographies

Grigory A Demyashkin, Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119048, Trubetskaya St., 8/2, Moscow, Russia

Doctor of Medical Sciences, Head of the Pathomorphology Department, National Medical Research Centre of Radiology, Moscow, Russia; Head of the Laboratory of Histology and Immunohistochemistry, ITMB, Sechenov University, Moscow, Russia

Mikhail A Parshenkov, Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University)

Researcher at Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya St., 8/2, Moscow 119048, Russia

Alibek A Tokov, Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119048, Trubetskaya St., 8/2, Moscow, Russia

Researcher at Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Trubetskaya St., 8/2, Moscow 119048, Russia

Tatiana P Sataieva, Department of Microbiology, Virology and Immunology, Crimean Federal University Named after V.I.Vernadsky, Order of the Red Banner of Labor Medical Institute Named after. S. I. Georgievsky, 295006, Lenina Blvd, 5/7, Simferopol, Russia

Head of the Department of Microbiology, Virology and Immunology, Crimean Federal University Named after V.I.Vernadsky, Order of the Red Banner of Labor Medical Institute Named after. S. I. Georgievsky

Ludmila A Shevkoplyas, Department of Microbiology, Virology and Immunology, Crimean Federal University Named after V.I.Vernadsky, Order of the Red Banner of Labor Medical Institute Named after. S. I. Georgievsky, 295006, Lenina Blvd, 5/7, Simferopol, Russia

Senior teacher of the Department of Microbiology, Virology and Immunology, Crimean Federal University Named after V.I.Vernadsky, Order of the Red Banner of Labor Medical Institute Named after. S. I. Georgievsky, Simferopol, Russia

Bekeev S Said, Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119048, Trubetskaya St., 8/2, Moscow, Russia

Phd researcher at the Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia

Gasanbekov N Shamil, Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), 119048, Trubetskaya St., 8/2, Moscow, Russia

Student at the Laboratory of Histology and Immunohistochemistry, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia

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