Therapeutic Efficacy of Plant-Based Hydrogels in Burn Wound Healing: Focus on Satureja Montana L. and Origanum Vulgare L.

Grigory A Demyashkin; Mikhail A Parshenkov; Alibek A Tokov; Tatiana P Sataieva; Ludmila A Shevkoplyas; Bekeev S Said; Gasanbekov N Shamil

doi:10.5937/scriptamed56-56050

Grigory A Demyashkin Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija https://orcid.org/0000-0001-8447-2600
Mikhail A Parshenkov Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija https://orcid.org/0009-0004-7170-8783
Alibek A Tokov Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija https://orcid.org/0009-0002-2583-9374
Tatiana P Sataieva Odeljenje za mikrobiologiju, virusologiju i imunologiju, Krimski federalni univerzitet V.I. Vernadski, Medicinski institut S.I. Georgijevski, nosilac Ordena Crvene zastave rada, 295006, Lenjinov bulevar 5/7, Simferopolj, Rusija https://orcid.org/0000-0001-6451-7285
Ludmila A Shevkoplyas Odeljenje za mikrobiologiju, virusologiju i imunologiju, Krimski federalni univerzitet V.I. Vernadski, Medicinski institut S.I. Georgijevski, nosilac Ordena Crvene zastave rada, 295006, Lenjinov bulevar 5/7, Simferopolj, Rusija https://orcid.org/0000-0003-1166-4585
Bekeev S Said Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija https://orcid.org/0000-0002-1841-4653
Gasanbekov N Shamil Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija https://orcid.org/0009-0007-3545-3965

DOI: https://doi.org/10.5937/scriptamed56-56050

Sažetak

Background/Aim: Burn injuries present a critical issue for healthcare systems worldwide. They often lead to numerous complications, including persistent inflammation, impaired healing and heightened infection risks. The growing challenge of antibiotic resistance further complicates treatment, making bacterial infections harder to control and wound healing less effective. Existing treatment methods are often limited, highlighting the need for alternative approaches. Aim of this study was to analyse influence of hydrolate-based gels derived from Satureja montana L. and Origanum vulgare L. on the healing of burn wounds.

Methods: New Zealand white rabbits (n = 25) with standardised Grade IIIa thermal burns were assigned to treatment groups receiving hydrolate-based gels (Satureja montana L. or Origanum vulgare L.), conventional therapy (Betadine (povidone-iodine) and Levomecol (methyluracil, chloramphenicol)), or no treatment. Inflammatory responses were tracked by collecting blood samples at the study’s begin, on day 3 and at one- and two-weeks post-burn, with subsequent ELISA analysis. Histological evaluation of tissue regeneration and inflammatory response was conducted using haematoxylin and eosin staining on days 3, 7 and 14.

Results: By day 14, Satureja montana L. demonstrated the most effective reduction in interleukin 6 (IL-6), tumour necrosis factor alpha (TNF-α) and C-reactive protein (CRP) levels, nearing baseline values. This biochemical improvement correlated with histological findings, showing advanced epithelial regeneration, reduced inflammatory cell infiltration and clearer dermal-epidermal boundaries. The results highlight Satureja montana L. as the most effective treatment in both inflammation control and tissue repair compared to Origanum vulgare L. and conventional therapy.

Conclusion: Hydrolate-based gels from Satureja montana L. and Origanum vulgare L. represent a promising option for adjunctive burn wound therapy. The Satureja montana L. group demonstrated significant improvements in inflammation control and tissue regeneration, while Origanum vulgare L. showed results comparable to standard therapy.

Biografije autora

Grigory A Demyashkin, Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija

Doktor medicinskih nauka, rukovodilac Odeljenja za patomorfologiju, Nacionalnog medicinskog istraživačkog centra za radiologiju, Moskva, Rusija; rukovodilac Laboratorije za histologiju i imunohistohemiju, ITMB, Sečenov Univerzitet, Moskva, Rusija

Mikhail A Parshenkov, Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija

Istraživač u Laboratoriji za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), Trubecka ul., 8/2, Moskva 119048, Rusija

Alibek A Tokov, Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija

Istraživač u Laboratoriji za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), Trubecka ul., 8/2, Moskva 119048, Rusija

Tatiana P Sataieva , Odeljenje za mikrobiologiju, virusologiju i imunologiju, Krimski federalni univerzitet V.I. Vernadski, Medicinski institut S.I. Georgijevski, nosilac Ordena Crvene zastave rada, 295006, Lenjinov bulevar 5/7, Simferopolj, Rusija

Šef Odeljenja za mikrobiologiju, virusologiju i imunologiju, Krimski federalni univerzitet V.I. Vernadski, Medicinski institut S.I. Georgijevski, nosilac Ordena Crvene zastave rada

Ludmila A Shevkoplyas, Odeljenje za mikrobiologiju, virusologiju i imunologiju, Krimski federalni univerzitet V.I. Vernadski, Medicinski institut S.I. Georgijevski, nosilac Ordena Crvene zastave rada, 295006, Lenjinov bulevar 5/7, Simferopolj, Rusija

Viši predavač Odeljenja za mikrobiologiju, virusologiju i imunologiju, Krimski federalni univerzitet V.I. Vernadski, Medicinski institut S.I. Georgijevski, nosilac Ordena Crvene zastave rada, Simferopolj, Rusija

Bekeev S Said, Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija

Doktorand na Laboratoriji za histologiju i imunohistohemiju, Prvi moskovski državni medicinski univerzitet I.M. Sečenov (Sečenov univerzitet), Moskva, Rusija

Gasanbekov N Shamil, Laboratorija za histologiju i imunohistohemiju, Prvi Moskovski državni medicinski univerzitet I.M. Sečenova (Sečenov Univerzitet), 119048, Trubecka ul., 8/2, Moskva, Rusija

Student na Laboratoriji za histologiju i imunohistohemiju, Prvi moskovski državni medicinski univerzitet I.M. Sečenov (Sečenov univerzitet), Moskva, Rusija

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