Mikrograft masnog tkiva u kosturu od plazma-gela kombinovan sa faktorima rasta iz trombocita u regeneraciji kožnih nabora
Sažetak
Background: The dermal aging process and the formation of deep wrinkles are a biological involution that also involves the regeneration system of cells immersed in the extracellular matrix and the papillary dermis. The progressive loss of niches of adult stem cells (MSCs) is more evident after the first third of life; it increases the phenotypic expression and the characteristics of the tissue senescence process. The purpose of this study was to clinically demonstrate that in viable micrograft there may be an improvement of deep wrinkles and surrounding tissues.
Methods: This study involved 11 female patients who underwent the correction of deep dermal wrinkles through a suspension containing 0.8 mL of viable micrografts in a 5 mL plasma gel scaffold, obtained from the centrifugation of a 20 cc venous sample peripheral blood, gelled by heat in a dry steriliser and the buffy coat coming from the same venous sample, in order verify overtime the improvement of the interested anatomical area. Individual signs of wrinkles and the degree of correction obtained for each treatment and each area were objectively evaluated by using a 10-0 visual analog scale (VAS), Modified Vancouver scale and Berardesca’s scale.
Results: With this technique excellent results were obtained. In fact, wrinkles were improved, as well as surrounding tissues, even after 60 days, as shown by the Berardesca’s, VAS and Modified Vancouver scales.
Conclusion: This retrospective clinical evaluation allowed us to consider the excellent clinical results obtained with this method for the treatment of deep wrinkles and surrounding tissues, through a suspension of progenitors with MSCs derived from adipose tissue (ADSCa) in a not inflammatory plasma gel scaffold combined with buffy coat.
Reference
2. Pontieri-Russo-Frati, General Pathology, IV Edition, I Reprint, Piccin Editore, Pages 282 and 631
3. Yan-Hua Rong , Gun-An Zhang, Cheng Wang, Fang-Gang Ning , Quantification of type I and III collagen content in normal human skin in different age groups , Zhonghua Shao Shang Za Zhi. 2008 Feb;24(1):51-3.
4. Jelaska A, et al. ; Fibroblast heterogeneity in physiological conditions and fibrotic disease. Springer Semin Immunopathol. 1999. PMID: 10945032 Review.
5. Svolacchia F, De Francesco F, Trovato L, Graziano A, Ferraro GA. An innovative regenerative treatment of scars with dermal micrografts. J Cosmet Dermatol. 2016 Jan 30.doi: 10.1111/jocd.12212. [Epub ahead of print].
6. Tonnard P, Verpaele A, Peeters G, Hamdi M, Cornelissen M, Declercq H. Nanofat grafting: basic research and clinical applications. Plast Reconstr Surg 2013 Oct;132(4):1017-26.
7. Svolacchia F, Svolacchia L. Microfiltered vs only disaggregated mesenchymal stem cells from adipose tissue in regenerative medicine. Scr. Med . 2020;51(3):152-7
8. Fabiano Svolacchia, Lorenzo Svolacchia Dermal Regeneration with MilliGraft® Kit of Nanofat: the Micrograft of Adipose Tissue. A Clinical Assessment Study . Scr Med 2019;50(3):117-21.
9. Amanda L Maisel-Campbell , Aliaa Ismail , Kelly A Reynolds , Emily Poon , Linda Serrano , Solomiya Grushchak , Carmen Farid 2, Dennis P West , Murad Alam , A systematic review of the safety and effectiveness of platelet-rich plasma (PRP) for skin aging , Arch Dermatol Res. 2020 Jul;312(5):301-315. doi: 10.1007/s00403-019-01999-6. Epub 2019 Oct 18.
10. Richard R Gammon , Dana Devine , Louis M Katz , Eva Quinley , YanYun Wu , Kathleen Rowe , Anna Razatos , Kyungyoon Min , Stefan Reichenberg , Roxane Smith , Buffy coat platelets coming to America: Are we ready? , Transfusion. 2020 Nov 10. doi: 10.1111/trf.16184. Online ahead of print.
11. Pengcheng Xu , Yaguang Wu , Lina Zhou , Zengjun Yang , Xiaorong Zhang , Xiaohong Hu , Jiacai Yang , Mingying Wang , Binjie Wang , Gaoxing Luo 4 5, Weifeng He, Biao Cheng , Platelet-rich plasma accelerates skin wound healing by promoting re-epithelialization , Burns Trauma . 2020 Aug 14;8:tkaa028. doi: 10.1093/burnst/tkaa028. eCollection 2020.
12. Svolacchia F, De Francesco F, Trovato L, Graziano A, Ferraro GA. An innovative regenerative treatment of scars with dermal micrografts. J Cosmet Dermatol. 2016 Jan 30.doi: 10.1111/jocd.12212. [Epub ahead of print].
13. Hass R, Kasper C, Böhm S, Jacobs R. Different populations and sources of human mesenchymal stem cells (MSC): A comparison of adult and neonatal tissue-derived MSC. Cell Commun Signal. 2011 May 14;9:12. doi: 10.1186/1478-811X-9-12.
14. Chan AK, Heathman TR, Coopman K, Hewitt CJ. Multiparameter flow cytometry for the characterisation of extracellular markers on human mesenchymal stem cells. Biotechnol Lett 2014;36(4):731-41
15. F.Svolacchia , L. Svolacchia , Correction of defects in the flaws of Bone TissueFacial, through the use of Tricalcium Phosphate or Calcium Hydroxyapatite with Plasma Gel. A Clinical Assessment Study . J. Appl. Cosmetol. 35,57-65 (July/December 2017 )
16. Karamichos D 1 Hutcheon AE Zieske JD Transforming growth factor β3 regulates assembly of a non fibrotic matrix in a 3D corneal model. J Tissue Eng Regen Med 2011 Aug 5 8 e 228 38 doi 10 1002 /term 429 Epub 2011 May 23
17. Xiangnan Zhao, Yue Liu, Pingping Jia, Hui Cheng, Chen Wang, Shang Chen, Haoyan Huang, Zhibo Han, Zhong-Chao Han, Zongjin Li , Chitosan Hydrogel-loaded MSC-derived Extracellular Vesicles Promote Skin Rejuvenation by Ameliorating the Senescence of Dermal Fibroblasts , Stem Cell Research & Therapy
18. Croce MA, Dyne K, Boraldi F, Quaglino D Jr, Cetta G, Tiozzo R, Pasquali Ronchetti I. ‘Hyaluronan affects protein and collagen synthesis by in vitro human skin fibroblasts’ Tissue Cell. 2001 Aug;33(4):326-31.
19. Song HF, Chai JK, Lin ZH, Liu NF, Chen ML, Zhao YZ, et al. Expression of hyaluronic acid and its receptor in the process of wound healing in different skin tissues and its significance. Zhonghua Yi Xue Za Zhi 2003; 83:1070–4.
20. Jing Qiao 1, Na An 2, Xiangying Ouyang 3 , Quantification of growth factors in different platelet concentrates , Platelets. 2017 Dec;28(8):774-778. doi: 10.1080/09537104.2016.1267338. Epub 2017 Feb 21.
21. Bi HS, Zhang C, Nie FF, Pan BL, Xiao E. Basic and Clinical Evidence of an Alternative Method to Produce Vivo Nanofat. Chin Med J (Engl). 2018 Mar 5;131(5):588-593. doi: 10.4103/0366-6999.226074.
22. Lo Furno D, Tamburino S, Mannino G, Gili E, Lombardo G, Tarico MS, Vancheri C, Giuffrida R, Perrotta RE. Nanofat 2.0: experimental evidence for a fat grafting rich in mesenchymal stem cells. Physiol Res. 2017 Sep 22;66(4):663-671. Epub 2017 Apr 12.
23. Pontieri-Russo-Frati, General Pathology, IV Edition, I Reprint, Piccin Editore cap. 15 Pag. 399-445
24. Kessiena L et al. ‘Hyaluronan in wound healing: Rediscovering a major player.’ Wound Rep Reg 2014;22:579-593.
25. Berardesca E, Distante F, Anthoine P, Rabbiosi G, Aubert L. Clinical and instrumental evaluation of the activity of an anti-wrinkle product on cutaneous relief and photoaged skin. J Appl Cosmetol. 1997;15:69–75.
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