Izolacija, karakterizacija i osjetljivost na antibiotike izolata stafilokoka sa posebnim osvrtom na meticilin-rezistentni Staphylococcus aureus iz nosa zdravstvenih radnika u tercijarnom zdravstvenom centru

S. aureus colonisation in healthcare workers

  • Mukul Chaurasia Senior Demonstrator
  • Neha Agrawal Senior Demonstrator
  • Ankita Chourasia Guest Faculty
  • Monica Bhatnagar Professor
  • Geeta Parihar Senior Professor
  • Vijaylatha Rastogi Professor
  • Amit Tak Project Scientist C (Medical)
Ključne reči: Obrazac osjetljivosti antibiotika, Zdravstveni radnici, Staphylococcus aureus otporan na meticilin, Minimalna inhibitorna koncentracija, Nozokomijalne infekcije

Sažetak


Background: Staphylococcus aureus (S. aureus) and its resistant form methicillin- resistant S. aureus (MRSA) is one of the most common nosocomial pathogens causing a wide range of infections in humans. The anterior nares are the main ecological niche for S. aureus. Nasal carriage of S. aureus acts as an important reservoir of infection among the colonised healthcare workers and they transmit the infection to the community. The aim of the present study was to estimate the nasal colonisation of S. aureus (with special reference to MRSA) in healthcare workers (doctors and nursing staff) and its antibiotic susceptibility pattern.

Methods: A descriptive study was planned in the Department of Microbiology, JLN Medical College, Ajmer (Rajasthan, India) after due approval from the institutional ethics committee. A total of 170 healthcare workers of either sex aged between 18 to 60 years were screened for S. aureus. Identification was done using standard microbiological techniques, by studying their morphology, colony and biochemical characteristics. MRSA was detected by cefoxitin disc diffusion test, oxacillin disc diffusion test, minimum inhibitory concentration (MIC) of oxacillin by E-test and oxacillin screen agar test. The observations were described in proportions and Chi-squared test was used to find independence. Statistical significance was considered at 5 %.

Results: Among 170 samples, 159 (93.53 %) samples (50 doctors and 109 nursing staff) had staphylococci colonisation. Among these 159 isolates, 34 (21.38 %) were S. aureus. Further, 8 (5.03 %) S. aureus isolates were resistant to both cefoxitin and oxacillin and had oxacillin MIC values ≥ 4 µg/mL and were considered MRSA. All the MRSA were detected in the nursing staff (males: 5.50 %, females: 1.83 %). All S. aureus and MRSA isolates were found sensitive to linezolid, vancomycin and mupirocin (minimum inhibitory concentration ≤ 4 µg/mL).

Conclusion: Screening and treatment of healthcare workers colonised with MRSA should be an important component of hospital infection control policy. These measures will prevent spread of infection to patients and the community and thereby reduce the morbidity, mortality and healthcare costs associated with nosocomial infections.

Biografije autora

Mukul Chaurasia, Senior Demonstrator

Department of Microbiology, JLN Medical College , Ajmer, Rajasthan, India

Neha Agrawal, Senior Demonstrator

Department of Microbiology, JLN Medical College, Ajmer, Rajasthan, India

Ankita Chourasia, Guest Faculty

Department of Microbiology, Maharshi Dayanand Sarawati University, Ajmer, Rajasthan, India

Monica Bhatnagar, Professor

Department of Microbiology, Maharshi Dayanand Saraswati Univerity, Ajmer, Rajasthan, India

Geeta Parihar, Senior Professor

Department of Microbiology, JLN Medical College, Ajmer, Rajasthan, India

Vijaylatha Rastogi, Professor

Department of Microbiology, JLN Medical College, Ajmer, Rajasthan, India

Amit Tak, Project Scientist C (Medical)

ICMR- National Centre for Disease Informatics and Research, Bengaluru, Karnataka, India

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2021/06/29
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