Correlation between conjunctival scraping cytology and other clinical dry eye metrics in determination of dry eye related inflammation

Bojana M Dačić Krnjaja; Jelena Potić; Danijela Raonić; Milenko Stojković

doi:10.2298/VSP171109185D

Bojana M Dačić Krnjaja University of Belgrade, Faculty of Medicine, Belgrade, SerbiaClinic for Eye Diseases, Clinical Center of Serbia, Belgrade, Serbia
Jelena Potić Clinic for Eye Diseases, Clinical Center of Serbia, Belgrade, Serbia
Danijela Raonić Clinical Centre of Montenegro, ‡Clinic for Eye Disease, Podgorica, Montenegro
Milenko Stojković University of Belgrade, Faculty of Medicine, Belgrade, SerbiaClinic for Eye Diseases, Clinical Center of Serbia, Belgrade, Serbia

DOI: https://doi.org/10.2298/VSP171109185D

Keywords: dry eye syndromes, conjunctiva, cytological techniques, sensitivity and specificity, diagnostic tests, routine, diagnosis, differential

Abstract

Abstract

Background/Aim. New and improved definition of dry eye disease (DED) emphasized that hiperosmolarity and inflammation with initial tear film instability play etiological role. The aim of this study was to explore relation of some commonly used clinical tests to dry eye disease (DED) related inflammation measured by conjunctival scraping cytology. Methods. We examined 100 subjects, 80 of them having DED. We performed Schirmer without anesthesia (Schirmer I), Fluorescein Tear Break Up Time (FTBUT), Rose Bengal (RB), Lid Parallel Conjunctival Folds (LIPCOF), Tear Meniscus Height (TMH) and Tear Ferning (TF) and compared the values to scraping scores of tarsal conjunctiva. Results. FTBUT had the best sensitivity (93.6%). The highest specificity was found with RB (93.2%), but it was also high with Schirmer I, TF and FTBUT (respectively 89.8%, 84.5%, 78.0%). RB and FTBUT had the highest correlation with conjunctival scraping score (r = 0.707, p < 0.001; r = -0.507, p < 0.001). Conclusion. In our study, FTBUT, though often used in many combinations of the DED tests, showed a remarkably high sensitivity and specificity on its own, as well as good correlation with DED related inflammation detected with conjunctival scraping cytology.

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