Redox status of pregnant women with thrombophilia

Aleksandra S. Dimitrijević; Jovana V.  Bradić; Vladimir I. Živković; Aleksandra B. Dimitrijević; Mirjana L. Milojević Čorbić; Janko M. Djurić; Dragan V. Vasiljević; Vladimir Lj. Jakovljević

doi:10.2298/VSP200908001D

Aleksandra S. Dimitrijević Institute of Public Health Kragujevac, Kragujevac, Serbia
Jovana V. Bradić University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, Serbia
Vladimir I. Živković University of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia
Aleksandra B. Dimitrijević University Clinical Center Kragujevac, Kragujevac, Serbia
Mirjana L. Milojević Čorbić University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia
Janko M. Djurić University Clinical Center Kragujevac, Kragujevac, Serbia
Dragan V. Vasiljević Institute of Public Health Kragujevac, Kragujevac, Serbia
Vladimir Lj. Jakovljević Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia

DOI: https://doi.org/10.2298/VSP200908001D

Keywords: antioxidants, oxidation-reduction, pregnancy, pregnancy trimesters, thrombophilia

Abstract

Background/Aim. Since the role of oxidative stress in the pathogenesis of thrombophilia in pregnancy has still not been clarified, the aim of the study was to assess the redox status of pregnant women with thrombophilia. Methods. The study involved 120 pregnant women divided into two groups: pregnant women with thrombophilia (n = 60) and women with normal pregnancy (n = 60). Blood samples for biochemical analysis were collected at the end of the first, second, and third trimester of pregnancy. Concentrations of hydrogen peroxide (H₂O₂), nitrites (NO₂^-), and the index of lipid peroxidation measured as thiobarbituric acid reactive substances (TBARS) were measured in plasma. Levels of reduced glutathione (GSH), activities of superoxide dismutase (SOD) and catalase (CAT) were measured in erythrocytes. Results. In women with thrombophilia, NO₂^- values were increased in the first and third trimester compared to healthy pregnant women (p < 0.05). The higher levels of TBARS and H₂O₂ were noticed in women with thrombophilia in the first trimester compared to healthy pregnant women (p < 0.05). The values of SOD and CAT were lower in women with thrombophilia in the third and GSH in the first trimester compared to the control group (p < 0.05). Conclusion. Our results suggest an increased generation of prooxidants in thrombophilia at the beginning of gestation, which declines as gestation progresses and reaches similar values as in normal pregnancy at the end of pregnancy. Generally viewed, pregnant women with thrombophilia was associated with impaired antioxidant capacity – activities of SOD and CAT were lower in the third and GSH in the first trimester compared to their values in healthy pregnant women.

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