Redox status of pregnant women with thrombophilia

Aleksandra S. Dimitrijević; Jovana V. Bradić; Vladimir I. Živković; Aleksandra B. Dimitrijević; Mirjana L. Milojević Čorbić; Janko M. Djurić; Dragan V. Vasiljević; Vladimir Lj. Jakovljević

doi:10.2298/VSP200908001D

Aleksandra S. Dimitrijević Institute of Public Health Kragujevac, Kragujevac, Serbia
Jovana V. Bradić University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, Serbia
Vladimir I. Živković University of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia
Aleksandra B. Dimitrijević University Clinical Center Kragujevac, Kragujevac, Serbia
Mirjana L. Milojević Čorbić University of Kragujevac, Faculty of Medical Sciences, Kragujevac, Serbia
Janko M. Djurić University Clinical Center Kragujevac, Kragujevac, Serbia
Dragan V. Vasiljević Institute of Public Health Kragujevac, Kragujevac, Serbia
Vladimir Lj. Jakovljević Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia

DOI: https://doi.org/10.2298/VSP200908001D

Ključne reči: antioksidansi, oksidoredukcija, trudnoća, trudnoća, tromesečja, trombofilija

Sažetak

Uvod/Cilj. Imajući u vidu da uloga oksidacionog stresa u patogenezi trombofilije u trudnoći nije u potpunosti razjašnjena, cilj rada je bio da se proceni redoks status trudnica sa trombofilijom. Metode. Studija je obuhvatila 120 trudnica koje su bile podeljene u dve grupe: grupu trudnica sa trombofilijom (n = 60) i grupu sa trudnoćom bez pridruženih bolesti (zdravim) (n = 60). Uzorci krvi za biohemijske analize su sakupljani na kraju prvog, drugog i trećeg trimestra trudnoće. U plazmi su određivane koncentracije vodonik peroksida (H₂O₂), nitrita (NO₂^-) i indeks lipidne peroksidacije meren kao reaktivne supstance tiobarbiturne kiseline (RSTBK). U lizatu eritrocita određivan je nivo redukovanog glutationa (GSH), aktivnosti superoksid dizmutaze (SOD) i katalaze (CAT). Rezultati. U grupi žena sa trombofilijom, vrednosti NO₂^- bile su povećane u prvom i trećem trimestru u poređenju sa zdravim trudnicama (p < 0,05). Viši nivoi RSTBK i H₂O₂ primećeni su kod žena sa trombofilijom u prvom trimestru, u poređenju sa zdravim trudnicama (p < 0,05). Vrednosti SOD i CAT bile su niže kod žena sa trombofilijom u trećem, a GSH u prvom trimestru trudnoće, u odnosu na kontrolnu grupu (p < 0,05). Zaključak. Naši rezultati ukazuju na to da je kod trudnica sa trombofilijom na početku trudnoće produkcija prooksidacionih parametara povećana, smanjuje se sa progresijom trudnoće i na kraju trudnoće dostiže vrednosti koje se beleže kod zdravih trudnica. Generalno posmatrano, trombofilija je bila povezana sa pogoršanjem antioksidacionog kapaciteta – aktivnosti SOD i CAT su bile niže u trećem, a nivo GSH u prvom trimestru, u poređenju sa njihovim vrednostima kod zdravih trudnica.

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Redoks status trudnica sa trombofilijom

Sažetak

Reference