Quantification of mast cells in different stages of periodontal disease

Dragan Marjanović; Zlatibor Anđelković; Zlata Brkić; Goran Videnović; Meliha Šehalić; Vladimir Matvijenko; Snežana Leštarević; Nadica Đorđević

doi:10.2298/VSP141222030M

Dragan Marjanović Dental Clinic, Faculty of Medicine, University of Kosovska Mitrovica, Kosovska Mitrovica, Serbia
Zlatibor Anđelković Institute for Histology and Embryology, Faculty of Medicine, University of Kosovska Mitrovica, Kosovska Mitrovica, Serbia
Zlata Brkić Dental Clinic, Military Medical Academy, Belgrade, Serbia; §Faculty of Medicine of the Military Medical Academy, University of Defence, Belgrade, Serbia
Goran Videnović Dental Clinic, Faculty of Medicine, University of Kosovska Mitrovica, Kosovska Mitrovica, Serbia
Meliha Šehalić Dental Clinic, Faculty of Medicine, University of Kosovska Mitrovica, Kosovska Mitrovica, Serbia
Vladimir Matvijenko Dental Clinic, Faculty of Medicine, University of Kosovska Mitrovica, Kosovska Mitrovica, Serbia
Snežana Leštarević Dental Clinic, Military Medical Academy, Belgrade, Serbia
Nadica Đorđević Dental Clinic, Faculty of Medicine, University of Kosovska Mitrovica, Kosovska Mitrovica, Serbia

DOI: https://doi.org/10.2298/VSP141222030M

Keywords: periodontal diseases, disease progression, mast cells,

Abstract

Background/Aim. Mast cells are mononuclear cells originating from bone marrow. They produce various biologically active substances, which allow them to actively participate in immune and inflammatory processes associated with periodontal disease. The study focused on distribution and density of mast cells in healthy gingiva as well as in different stages of periodontal disease. Methods. The material used for this purpose was gingival biopsies taken from 96 patients classified into 4 groups: healthy gingiva, gingivitis, initial and severe periodontal disease. Toluidine blue staining according to Spicer was utilized for identifying mast cells. Results. Basing on our study, the density of mast cells in the gingival tissue increases with the progression of the infection, which means they are more numerous in gingivitis compared to healthy gingiva, as well as in periodontal disease compared to gingivitis. Conclusion. Increase in the number of mast cells in the infected gingiva can be correlated with an increased influx of inflammatory cells from blood circulation into the gingival stroma, as well as with the collagen lysis, since these cells produce substances with collagenolytic potential. Based on the distribution of mast cells, it could be concluded that in the evolution of periodontal disease there are significant dynamic alterations in migration and localization of these cells.

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