Disturbance of oxidative balance in the first trimester of spontaneous abortions

Zorica Grujić; Ilija Grujić; Mirjana Bogavac; Aleksandra Nikolić; Radoslav Mitić; Zoran Stajić

doi:10.2298/VSP150321123G

Zorica Grujić Department of Obstetrics and Gynecology, Clinical Center of Vojvodina, Novi Sad, Serbia;Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
Ilija Grujić Private Gynecological Hospital “Femina”, Novi Sad, Serbia
Mirjana Bogavac Department of Obstetrics and Gynecology, Clinical Center of Vojvodina, Novi Sad, Serbia;Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
Aleksandra Nikolić Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
Radoslav Mitić Institute of Pharmacology, Faculty of Medicine, University of Priština, Kosovska Mitrovica, Seriba
Zoran Stajić Division of Cardiology, Department of Internal Medicine, Institute of Health Protection of the Ministry of Inferior, Belgrade, Serba

DOI: https://doi.org/10.2298/VSP150321123G

Keywords: oxidative stress, abortion, spontaneous, lipid peroxidation, superoxide dismutase, catalase, glutathione, glutathione peroxidase, gravidity,

Abstract

Background/Aim. Pregnancy is defined as a condition of increased oxidative stress. The aim of this research was to determine the intensity of pro-oxidative processes and the content of GSH, as well as antioxidative enzymes: superoxide dismutase (SOD), catalase (CAT), gluthatione peroxidase (GSH-Px), and the total antioxidative status (TAS) in patients with spontaneous abortions. Methods. A total of 120 patients were involved in the research (70 spontaneous abortions and 50 healthy pregnancies). The patients were divided into groups: 35 patients with incomplete and complete spontaneous abortion (group S), 35 patients with missed abortion (group M) and a control group of 50 healthy pregnancies (group N), all of them being in the first trimester of pregnancy. The intensity of lipid proxidation (LPx) was determined with a modified thyobarbituric acid method. The GSH content in erythrocytes was determined by the method based on the amount of non-protein sulfhydryl residues using the Ellman's reagens. The following antioxidative parameters in the blood were measured: SOD – by the method with xanthine oxidase-using commercial RANSOD sets; CAT – by the method of Aebi (the enzyme activity was measured by monitoring the decomposition of H₂O₂ at 240 nm); GSH-Px was determined using hydrogen peroxide as a substrate. The TAS was determined using the ferric reducing autioxidant potential (FRAP) method. Results. The highest average value of LPx was recorded in the spontaneous abortion group (48.03 pmoL/mg Hgb), and the lowest value was recorded in the control group (26.06 pmoL/mg Hgb). A statistically significant positive correlation between LPx and CAT in the group of patients with missed abortion was also noted (p < 0.05, r = 0.37). There was a statistically highly significant difference (p < 0.001) in SOD and in CAT activitices between the examined patients (groups S and N) and the control group (Student’s t-test and ANOVA). The highest average value of TAS was recorded in the group S (710.39 µmol/L), while the value in the group M was 277.66 µmol/L. The average value of TAS in the control group was 452.12 µmol/L. Student’s t-test showed a statistically highly significant difference in the values of TAS between the examined patients (groups S and M) and the control group. Conclusion. Determination of the value of pro-oxidative and antioxidative parameters in patients with spontaneous abortion can be the indicator of condition of fetoplacental unit and these analyses can be included in the protocol of the rutine perinatal diagnostics.

Author Biographies

Zorica Grujić, Department of Obstetrics and Gynecology, Clinical Center of Vojvodina, Novi Sad, Serbia;Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia

No

Mirjana Bogavac, Department of Obstetrics and Gynecology, Clinical Center of Vojvodina, Novi Sad, Serbia;Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia

Department of Obstetrics and Gynecology

Aleksandra Nikolić, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia

Department of Obstetrics and Gynecology-Urgent laboratory

Radoslav Mitić, Institute of Pharmacology, Faculty of Medicine, University of Priština, Kosovska Mitrovica, Seriba

Institute of Pharmacology

Zoran Stajić, Division of Cardiology, Department of Internal Medicine, Institute of Health Protection of the Ministry of Inferior, Belgrade, Serba

Department of Internal Medicine

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