Poremećaj redoks ravnoteže kod žena sa spotanim pobačajima u prvom trimestru trudnoće

  • Zorica Grujić Department of Obstetrics and Gynecology, Clinical Center of Vojvodina, Novi Sad, Serbia;Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
  • Ilija Grujić Private Gynecological Hospital “Femina”, Novi Sad, Serbia
  • Mirjana Bogavac Department of Obstetrics and Gynecology, Clinical Center of Vojvodina, Novi Sad, Serbia;Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
  • Aleksandra Nikolić Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
  • Radoslav Mitić Institute of Pharmacology, Faculty of Medicine, University of Priština, Kosovska Mitrovica, Seriba
  • Zoran Stajić Division of Cardiology, Department of Internal Medicine, Institute of Health Protection of the Ministry of Inferior, Belgrade, Serba
DOI: https://doi.org/10.2298/VSP150321123G
Ključne reči: oxidative stress||, ||stres, oksidativni, abortion, spontaneous||, ||abortus, spontani, lipid peroxidation||, ||lipidi, peroksidacija, superoxide dismutase||, ||peroksid dismutaza, catalase||, ||katalaza, glutathione||, ||glutation, glutathione peroxidase||, ||glutation peroksidaza, gravidity||, ||trudnoća.,

Sažetak


Uvod/Cilj. Trudnoća se definiše kao stanje povišenog oksidativnog stresa. Cilj rada bio je da se odredi intenzitet prooksidativnih procesa i sadržaja glutationa (GSH), kao i antioksidativnih enzima: superoksid dismotaze (SOD), katalaze (CAT), glutation peroksidaze (GSH-Px) i totalnog antioksidativnog statusa (TAS) kod žena sa spontanim pobačajem. Metode. U istraživanje je bilo uključeno 120 žena (70 sa spontanim pobačajem i 50 zdravih trudnica). Žene su bile podeljene u grupe: 35 žena sa nekompletnim i kompletnim spontanim pobačajem (grupa S), 35 žena sa missed abortusom (grupa M) i kontrolna grupa od 50 zdravih trudnica (grupa N) u prvom trimestru trudnoće. Intenzitet lipidne peroksidacije (Lpx) određivan je modifikovanom metodom sa tiobarbiturnom kiselinom. Sadržaj GSH u eritrocitima određivan je na osnovu količine neproteinskih sulfhidrilnih ostataka pomoću Ellman-ovog reagensa. Od antioksidativnih parametara u krvi vršeno je određivanje: SOD – metodom sa ksantin oksidazom, komercijalnim setovima RANSOD; CAT – Aebi-jevom metodom (aktivnost enzima merila se praćenjem razlaganja H2O2 na 240 nm); aktivnost GSH-Px određivana je pomoću vodonik-peroksida kao supstrata. TAS je određivan sa ferric reducing antioxidant potential (FRAP) metodom. Resultati. Najveća prosečna vrednost LPx zabeležena u grupi žena sa spontanim pobačajem (grupa S), 48,03 pmoL/mg Hgb, a najniža vrednost zabeležena je u kontrolnoj grupi (26,06 pmoL/ mg Hgb). Takođe, zapaženo je postojanje pozitivne korelacije između LPx i CAT (p < 0,05, r = 0,37) u grupi žena sa missed abortusom (grupa M). Postoji statistički visokoznačajna razlika (p < 0,001) u vrednostima SOD i CAT između žena u grupama S i M i kontrolne grupe (t-test i ANOVA). Najviša prosečna vrednost TAS zabeležena je u grupi S (710,39 µmol/L), dok je vrednost u grupi M bila 277,66 µmol/L. Vrednosti TAS u kontrolnoj grupi zdravih trudnica iznosila je 452,12 µmol/L. Vrednosti t-testa i ANOVA pokazuju da postoji statistički visokoznačajna razlika u vrednostima TAS između žena sa abortusom (grupe S i M) i kontrolne grupe. Zaključak. Određivanje vrednosti prooksidativnih i antioksidativnih parametara kod žena sa spontanim pobačajem može biti pokazatelj stanja fetoplacentarne jedinice i ove analize u budućnosti mogu biti uvrštene u protokol rutinske prenatalne dijagnostike.

Biografije autora

Zorica Grujić, Department of Obstetrics and Gynecology, Clinical Center of Vojvodina, Novi Sad, Serbia;Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
No
Mirjana Bogavac, Department of Obstetrics and Gynecology, Clinical Center of Vojvodina, Novi Sad, Serbia;Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
Department of Obstetrics and Gynecology
Aleksandra Nikolić, Faculty of Medicine, University of Novi Sad, Novi Sad, Serbia
Department of Obstetrics and Gynecology-Urgent laboratory
Radoslav Mitić, Institute of Pharmacology, Faculty of Medicine, University of Priština, Kosovska Mitrovica, Seriba
Institute of Pharmacology
Zoran Stajić, Division of Cardiology, Department of Internal Medicine, Institute of Health Protection of the Ministry of Inferior, Belgrade, Serba

kardiolog / interventni i neinterventni

Reference

Gupta S, Agarwal A, Banerjee J, Alvarez JG. The role of oxidative stress in spontaneous abortion and recurrent pregnancy loss: A systematic review. Obstet Gynecol Surv 2007; 62(5): 335−47.

Grujić Z, Grujić I, Bogavac M, Nikolić A. The role of oxidative stress in spontaneous abortions.11th World Congress of Perinatal Medicine; Moscow, Russia; 2013 June 19-23; Abstracts. J Perinatal Med 2013; 41(Suppl 1): 525.

Tang C, Liang J, Qian J, Jin L, Du M, Li M, et al. Opposing role of JNK-p38 kinase and ERK1/2 in hydrogen peroxide-induced oxidative damage of human trophoblast-like JEG-3 cells. Int J Clin Exp Pathol 2014; 7(3): 959−68.

Hubel CA, Roberts JM, Taylor RN, Musci TJ, Rogers GM, McLaughlin MK. Lipid peroxidation in pregnancy: New perspectives on preeclampsia. Am J Obstet Gynecol 1989; 161(4): 1025−34.

Little RE, Gladen BC. Levels of lipid peroxides in uncomplicated pregnancy: A review of the literature. Reprod Toxicol 1999; 13(5): 347−52.

Sugino N, Takiguchi S, Umekawa T, Heazell A, Caniggia I. Oxidative stress and pregnancy outcome: A workshop report. Placenta 2007; 28: 48−50.

Burton GJ, Jauniaux E. Placental oxidative stress: from miscarriage to preeclampsia. J Soc Gynecol Investig 2004; 11(6): 342−52.

Grujic Z, Grujic I, Bogavac M. Oxidative stress and spontaneous abortion. J Matern Fetal Neonatal Med 2014; 27(S1): 1−437.

Sugino N, Shimamura K, Takiguchi S, Tamura H, Ono M, Nakata M, et al. Changes in activity of superoxide dismutase in the human endometrium throughout the menstrual cycle and in early pregnancy. Hum Reprod 1996; 11(5): 1073−8.

Sugino N, Nakamura Y. Change in activities od SOD and LP in corpus luteum during pregnancy in rats. J Reprod Fertil 1993; 97: 347−51

Đorđević BV, Pavlović DD, Kocić MG. Biochemistry free radicals.Niš: Sirius-Print; 2000. p. 5−25. (Serbian)

Nikolić Đorđević A. Biochemical markers of adverse pregnancy outcomes in fetal blood. Yugoslav Med Biochem 2006; 25(4): 391−6.

Morley S, Shillito J, Tang T. Preventing miscarriage of unknown aetiology. Obstet Gynecol 2013; 15: 99−105.

Slater TF. Overview of methods used for detecting lipid peroxidation. Meth Enzymol 1984; 105: 283−93.

Kapetanovic IM, Mieyal JJ. Inhibition of acetaminophen-induced hepatotoxicity by phenacetin and its aloxy analogs. J Pharmacol Exp Ther 1979; 209(1): 25−30.

McCord JM, Fridovich I. Superoxide dismutase: An enzymic function for erythrocuprein (hemocuprein). J Biol Chem 1969; 244(22): 6049−55.

Aebi H. Catalase in vitro. Meth Enzymol 1984; 105: 121−6.

Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clin Med 1967; 70(1): 158−69.

Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of "antioxidant power": The FRAP assay. Anal Biochem 1996; 239(1): 70−6.

Đukić M. Lipid peroxidation induced by free radicals. In: Đukić M, editor. Oxidative stress-clinical diagnostical significance.Belgrade: Mono and Manjana; 2008. p. 3−19. (Serbian)

Smith L. Cholesterol oxidation. In: Pelfrey VC, editor. Membrane lipid oxidation. Boca Ration, FL: CRC Press; 1990. p. 130−54.

Kanias T, Wong K, Acker JP. Determination of lipid peroxidation in desiccated red blood cells. Cell Preserv Technol 2007; 5(3): 165−74.

Ozkaya O, Sezik M, Kaya H. Serum malondialdehyde, erythrocyte glutathione peroxidase and erythrocyte superoxide dismutase levels in women with early spontaneous abortions accompanied by vaginal bleeding. Med Sci Monit 2008; 14(1): 47−51.

Simşek M, Naziroğlu M, Simşek H, Cay M, Aksakal M, Kumru S. Blood plasma levels of lipoperoxides, glutathione peroxidase, beta carotene, vitamin A and E in women with habitual abortion. Cell Biochem Funct 1998; 16(4): 227−31.

Sane AS, Chokshi SA, Mishra VV, Barad DP, Shah VC, Nagpal S. Serum lipoperoxides in induced and spontaneous abortions. Gynecol Obstet Invest 1991; 31(3): 172−5.

Canadanovic-Brunet JM, Djilas SM, Cetkovic GS, Tumbas VT, Mandic AI, Canadanovic VM. Antioxidant activities of different Teucrium montanum L. Extracts. Int J Food Sci Technol 2006; 41(6): 667−73.

Rudov A, Balduini W, Carloni S, Perrone S, Buonocore G, Albertini MC. Involvement of miRNAs in placental alterations mediated by oxidative stress. Oxid Med Cell Longev 2014; 2014: 103068.

Wernerman J, Luo JL, Hammarqvist F. Glutathione status in critically-ill patients: Possibility of modulation by antioxidants. Proc Nutr Soc 1999; 58(3): 677−80.

Blanco-Muñoz J, Aguilar-Garduño C, Gamboa-Avila R, Rodríguez-Barranco M, Pérez-Méndez O, Huesca-Gómez C, et al. Association between PON1 genetic polymorphisms and miscarriage in Mexican women exposed to pesticides. Sci Total Environ 2013; 449: 302−8.

Biri A, Kavutcu M, Bozkurt N, Devrim E, Nurlu N, Durak I. Investigation of free radical scavenging enzyme activities and lipid peroxidation in human placental tissues with miscarriage. J Soc Gynecol Investig 2006; 13(5): 384−8.

Prokopenko VM, Partsalis GK, Pavlova NG, Burmistrov SO, Arutyunyan AV. Glutathione-dependent system of antioxidant defense in the placenta in preterm delivery. Bull Exp Biol Med 2002; 133(5): 442−3.

Sugino N, Nakata M, Kashida S, Karube A, Takiguchi S, Kato H. Decreased superoxide dismutase expression and increased concentrations of lipid peroxide and prostaglandin F(2alpha) in the decidua of failed pregnancy. Mol Hum Reprod 2000; 6(7): 642−7.

Zachara BA, Dobrzyński W, Trafikowska U, Szymański W. Blood selenium and glutathione peroxidases in miscarriage. BJOG 2001; 108(3): 244−7.

Aksoy AN, Aksoy H, Ozturk N, Bulut C. Erythrocyte TAO and TBARS levels in patients who suffered missed miscarriage. Turk J Med Sci 2009; 39(1): 1−5.

Panzan MQ, Mattar R, Maganhin CC, Simoes RS, Rossi AG, da Motta AE, et al. Evaluation of FAS and caspase-3 in the endometrial tissue of patients with idiopathic infertility and recurrent pregnancy loss. Eur J Obstet Gynecol Rep Biol 2012; 167(1): 12−6.

Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol 2005; 3(1): 28.

Milašinović LJ, Hrabovski I, Grujić Z, Bogavac M, Nikolić A. Biochemical and physiological characteristics of neonates born to mothers with diabetes during gestation. J Med Biochem 2012; 31(1): 47−52.

Deveer R, Deveer M, Engin-Üstün Y, Akbaba E, Uysal S, Sarikaya E, et al. Role of oxidative stress on vaginal bleeding during the first trimester of pregnant women. Int J Fertil Steril 2014; 7(4): 271−4.

Objavljeno
2017/03/13
Broj časopisa
Vol. 73 Br. 11 (2016)
Rubrika
Originalni članak