Uticaj vodenog i metanolnog ekstrakta stabla morača (Foeniculum vulgare Miller) na kontraktilnost izolovanog tankog creva pacova
Sažetak
Uvod/Cilj. Morač (Foeniculum vulgare Miller, Apiaceae) se od davnina koristi u tradicionalnoj medicini kao karminativ. Ova studija je imala za cilj da istraži efekte vodenog i metanolnog ekstrakta stabla morača na aktivnost tankog creva. Metode. Relaksantna aktivnost vodenog i metanolnog ekstrakta morača ispitivana je in vitro pomoću tri eksperimentalna modela na izolovanom ileumu pacova: spontana aktivost, acetilholinom i kalijum-holridom (KCl)-indukovane kontrakcije. Kvalitativni sastav vodenog i metanolnog ekstrakta morača određivan je pomoću tečne hormotografije visokih performansi (HPLC). Rezultati. U prisustvu vodenog ekstrakta stabla morača u koncentraciji od 3 mg/mL maksimalna inhibicija spontanih kontrakcija izolovanog ileuma pacova je iznosila 35.05% ± 3.57%, dok je u prisustvu metanolnog ekstrakta stabla morača iste koncentracije maksimalna redukcija spontanih kontrakcija iznosila 48.91% ± 6.31%. Ekstrakti stabla morača proporcionalno primenjenoj koncentraciji značajno su inhibisali kontrakcije ileuma stimulisane acetilholinom i kalijum-hloridom (p < 0.01). U metanolnom ekstraktu morača utvrđeno je prisustvo 3-kafeoilhine kiseline, hlorogenske kiseline, 4-kafeoilhine kiseline, 1,3-dikafeoilhine kiseline, rutina, mikvelianina, kvercetin heterozida, 1,5-dikafeoilhine kiseline, 1,4-dikafeoilhine kiseline, apigenina i rozmarinske kiseline. U vodenom ekstraktu njihovo prisustvo je nađeno u tragovima. Zaključak. Rezultati ove studije potvrđuju da vodeni i metanolni ekstrakti stabla morača deluju spazmolitički na glatke mišiće tankog creva i mogu se koristiti za kontrolu intestinalne motorike.
Reference
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