Antibakterijski efekti novih endodontskih materijala na bazi kalcijum silikata

  • Dijana Trišić University of Belgrade, Faculty of Dental Medicine, Belgrade, Serbia
  • Bojana Ćetenović University of Belgrade, Institute for Nuclear Sciences “Vinča“, Belgrade, Serbia
  • Nemanja Zdravković University of Belgrade, Faculty of Veterinary Medicine, Belgrade, Serbia
  • Tatjana Marković University of Belgrade, Institute for Medicinal Plants Research “Dr Josif Pančić”, Belgrade, Serbia
  • Biljana Dojčinović University of Belgrade, Institute of Chemistry, Technology and Metallurgy, Belgrade, Serbia
  • Vukoman Jokanović University of Belgrade, Institute for Nuclear Sciences “Vinča“, Belgrade, Serbia
  • Dejan Marković University of Belgrade, Faculty of Dental Medicine, Belgrade, Serbia
DOI: https://doi.org/10.2298/VSP161231130T
Ključne reči: zub, bolesti pulpe;, zub, lečenje korenskog kanala;, kalcijum silikat;, kalcijum hidroksid;, antiinfektivi.

Sažetak


Uvod/Cilj. Osnovni cilj endodonskog lečenja je eliminacija patološki izmenjenog tkiva, eliminacija infekcije korensko kanala i njegovo hermetičko trodimenzionalno zatvaranje. Cilj istraživanja je bio da se proceni antibakterijska aktivnost novih endodontskih nano-strukturiranih materijala na bazi visoko aktivnih kalcijum silikata albo-mineral polyoxide carbonate aggregate (ALBO-MPCA) i calcium silicates (CS) u odnosu na mineral trioxide aggregate (MTA+) i UltraCal XS (CH). Metode. Testirana je antibakterijska aktivnost materijala protiv Staphylo­coccus aureus (ATCC 25923) i Enterococcus faecalis (ATCC 14506), kao i kliničkih izolata: Rothia dentocariosa, Enterococcus faecalis, Staphylococcus aureus, Streptococcus anginosus i Streptococcus vestibularis pomoću agar difuzionog testa. Merenja pH vrednosti obavljena su korišćenjem pH metra. Ukupan iznos oslobođenih jona određivan je pomoću ICP-OES. Rezultati. Svi testirani materijali pokazali su najbolji antibakterijski efekat nakon 1 h od inkubacije. Nakon 3 h i 24 h od inkubacije, antibakterijski efekat svih testiranih materijala bio je sličan. Agar difuzioni test pokazao je da materijali ALBO-MPCA, CS i MTA+ ispoljavaju slične zone inhibicije rasta (p > 0.05) osim u slučaju Staphylococcus aureus, gde je materijal ALBO-MPCA pokazao bolje antibakterijsko dejstvo nego MTA+ nakon 3 h i 24 h od inkubacije (p < 0.05). Nakon 24 h od inkubacije, zone inhibicije su bile najizraženije u slu­čaju materijala CS protiv Staphylococcus aureus (16.67 ± 2.34 mm), zatim ALBO-MPCA (14.67 ± 1.21 mm), a najslabije u slučaju CS protiv Enterococcus faecalis (6.50±1.76 mm). Materijal CH ispoljio je najveću pH vrednost, zatim ALBO-MPCA, CS i MTA+. Zaključak. Ispoljeni antibakterijski efekti ukazuju na to da materijali na bazi visoko aktivnih kalcijum silikata mogu da predstavljaju efikasnu zamenu za MTA u terapiji zuba sa nezavršenim rastom korena u jednoj poseti, te se stoga preporučuju za dalja klinička ispitivanja.

Reference

Vera J, Siqueira JF Jr, Ricucci D, Loghin S, Fernández N, Flores B et al. One-versus two-visit endodontic treatment of teeth with apical periodontitis: a histobacteriologic study. J Endod 2012; 38(8): 1040‒52.

Endo MS, Ferraz CC, Zaia AA, Almeida JF, Gomes BP. Quanti-tative and qualitative analysis of microorganisms in root-filled teeth with persistent infection: monitoring of the endodontic retreatment. Eur J Dent 2013; 7(3): 302‒9.

Peciuliene V, Reynaud AH, Balciuniene I, Haapasalo M. Isolation of yeasts and enteric bacteria in root-filled teeth with chronic apical periodontitis. Int Endod J 2001; 34(6): 429‒34.

Heyder M, Kranz S, Völpel A, Pfister W, Watts DC, Jandt KD et al. Antibacterial effect of different root canal sealers on three bacterial species. Dent Mater 2013; 29(5): 542‒9.

Mohammadi Z, Dummer PM. Properties and applications of cal-cium hydroxide in endodontics and dental traumatology. Int Endod J 2011; 44(8): 697‒730.

Evens M, Davies JK, Sundqvist G, Fridor D. Mechanisms in-volved in the resistance of Enterococcus faecalis to calcium hydroxide. Int Endod J 2001; 35(3): 221‒8.

Cetenovic B, Markovic D, Petrovic B, Peric T, Jokanovic V. Use of mineral trioxide aggregate in the treatment of traumatized teeth in children–Two case reports. Vojnosanit Pregl 2013; 70(8): 781‒4.

Camilleri J, Sorrentino F, Damidot D. Investigation of the hydra-tion and bioactivity of radiopacified tricalcium silicate ce-ment, Biodentine and MTA Angelus. Dent Mater 2013; 29(5): 580‒93.

Sisli SN, Ozbas H. Comparative Micro-computed Tomo-graphic Evaluation of the Sealing Quality of ProRoot MTA and MTA Angelus Apical Plugs Placed with Various Tech-niques. J Endod 2017; 43(1): 147‒51.

Tanomaru-Filho M, Tanomaru JM, Barros DB, Watanabe E, Ito IY. In vitro antimicrobial activity of endodontic sealers MTA-based cements and Portland cement. J Oral Sci 2007; 49(1): 41‒5.

Flores-Ledesma A, Barceló Santana F, Bucio L, Arenas-Alatorre JA, Faraji M, Wintergerst AM. Bioactive materials improve some physical properties of a MTA-like cement. Mater Sci Eng C Mater Biol Appl 2017; 71: 150‒5.

Duarte MA, Alves de Aguiar K, Zeferino MA, Vivan RR, Ordi-nola-Zapata R, Tanomaru-Filho M et al. Evaluation of the pro-pylene glycol association on some physical and chemical prop-erties of mineral trioxide aggregate. Int Endod J 2012;45(6): 1-6.

Khalil I, Naaman A, Camilleri J. Properties of Tricalcium Sili-cate Sealers. J Endod 2016; 42(10): 1529‒35.

Jokanovic V, Colovic B, Mitric M, Markovic D, Cetenovic B. Syn-thesis and properties of a new endodontic material based on nanostructured highly active calcium-silicates and calcium carbonates. Int J Appl Ceram Techol 2014; 11(1): 57‒64.

Opacic-Galic V, Petrovic V, Zivkovic S, Jokanovic V, Nikolic B, Knezevic-Vukcevic J, et al. New nanostructural biomaterials based on active silicate systems and hydroxyapatite: character-ization and genotoxicity in human peripheral blood lympho-cytes. Int Endod J 2012; 46(6): 506‒16.

Leonardo MR, Silva LA, Tanomaru-Filho M, Bonifacio KC, Ito IY. In vitro evaluation of antimicrobial activity of sealers and pastes used in endodontics. J Endod 2000; 26(7): 391‒4.

Zhang H, Pappen FG, Haapasalo M. Dentin enhances the anti-bacterial effect of Mineral Trioxide Aggregate and BioAggre-gate. J Endod 2009; 35(2): 221‒4.

Eldeniz AU, Hadimli HH, Ataoglu H, Orstavik D. Antibacterial effect of selected root end filling materials. J Endod 2006; 32(4): 345‒9.

Vivan RR, Zapata RO, Zeferino MA, Bramante CM, Bernardineli N, Garcia RB, et al. Evaluation of the physical and chemical properties of two commercial and three experimental root-end filling materials. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 110(2): 250‒6.

Begue WJ, Kline RM. The use of tetrazolium salts in bioauto-graphic procedures. J Chromatogr 1972; 64(1): 182‒4.

Lovato KF, Sedgley CM. Antibacterial activity of endosequence root repair material and ProoRoot MTA against clinical iso-lates of Enterococcus faecalis. J Endod 2011; 37(11): 1542‒6.

Mohammadi Z, Giardiono L, Palazzi F, Shalavi S. Antibacterial activity of a new mineral trioxide aggregate-based root canal sealer. Int Dent J 2012; 62(2): 70‒3.

Kim D, Kim E. Antimicrobial effect of calcium hydroxide as an intracanal medicament in root canal treatment: a literature review-Part I. In vitro studies. Restor Dent Endod 2014; 39(4): 241‒52.

McHung CP, Zhang P, Michalek S, Eleazer PD. pH required to kill Enterococcus faecalis in vitro. J Endod 2004; 30(4); 218‒9.

Zancan RF, Vivan RR, Milanda Lopes MR, Weckwerth PH, de Andrade FB, Ponce JB, et al. Antimicrobial Activity and Physi-cochemical Properties of Calcium Hydroxide Pastes Used as Intracanal Medication. J Endod 2016; 42(12): 1822‒8.

Kim RJ, Kim MO, Lee KS, Lee DY, Shin JH. An in vitro evalu-ation of the antibacterial properties of three mineral trioxide aggregate (MTA) against five oral bacteria. Arch Oral Biol 2015; 60(10): 1497‒502.

Bhavana V, Chaitanya KP, Gandi P, Patil J, Dola B, Reddy RB. Evaluation of antibacterial and antifungal activity of new cal-cium-based cement (Biodentine) compared to MTA and glass ionomer cement. J Conserv Dent 2015; 18(1): 44‒6.

Özyürek Tand, Demiryürek EÖ. Comparison of the antimicrobi-al activity of direct pulp-capping materials: Mineral trioxide aggregate-Angelus and Biodentine. J Conserv Dent 2016; 19(6): 569‒72.

Asgary S, Kamrani FA. Antimicrobial effect of five different root canal sealing materials. J Oral Sci 2008; 50(4): 469‒4.

Holt DM, Watts D, Beeson TJ, Kirkpatrick TC, Rutledge RE. The anti-microbial effect against enterococcus faecalis and the compressive strength of two types of mineral trioxide aggre-gate mixed with sterile water or 2% chlorhexidine liquid. J Endod 2007; 33(7): 844‒7.

Sipert CR, Hussne RP, Nishiyama CK, Torres SA. In vitro anti-microbial activity of Fill Canal, Sealapex, Mineral Trioxide Aggregate, Portland cement and EndoRez. Int Endod J 2005; 38(8): 539‒43.

Ribeiro CS, Scelza MF, Hirata JR, de Oliveira LM. The antimi-crobial activity of gray-colored mineral trioxide aggregate (GMTA) and white-colored MTA (WMTA) under aerobic and anaerobic conditions. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2010; 109(6): e109‒12.

Yasuda Y, Kamaguchi A, Saito T. In vitro evaluation of the an-timicrobial activity of a new resin-based endodontic sealer against endodontic pathogens. J Oral Sci 2008; 50(3): 309‒13.

Miyagak DC, de Carvalho EM, Robazza CR, Chavasco JK, Levora-to GL. In vitro evaluation of the antimicrobial activity of en-dodontic sealers. Braz Oral Res 2006; 20(4): 303‒6.

Mukherjee A, Sadiq IM, Prathna TC, Chandrasekaran N. Antimi-crobial activity of aluminium oxide nanoparticles for potential clinical applications. Formatex Res Center 2012; 1: 245‒51.

D'Amato RF, Thornsberry C. Calcium and Magnesium in Mueller-Hinton Agar and Their Influence on Disk Diffusion Susceptibility Results. Curr Microbiol 1972; 2(3): 135‒8.

Objavljeno
2021/02/10
Broj časopisa
Vol. 76 Br. 4 (2019): Vojnosanit pregl
Rubrika
Originalni članak