The concentration of matrix metalloproteinase 9 in the tumor and peritumoral tissue as a prognostic marker in the breast cancer patients

Danijela M. Cvetković; Bojan Z. Milošević; Aleksandar M. Cvetković; Srdjan M. Ninković; Jovana V. Jovankić; Dalibor V. Jovanovic; Snežana D. Marković

doi:10.2298/VSP170313118C

Danijela M. Cvetković Danijela Cvetković, MSci Laboratory for Cell and Molecular Biology Department for Biology and Ecology Faculty of Science, University of Kragujevac Radoja Domanovica 12, 34000 Kragujevac, Serbia
Bojan Z. Milošević University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, Serbia
Aleksandar M. Cvetković University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, Serbia
Srdjan M. Ninković University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, Serbia
Jovana V. Jovankić University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, Serbia
Dalibor V. Jovanovic University of Kragujevac, Faculty of Medical Sciences, Department of Pathology, Kragujevac, Serbia
Snežana D. Marković University of Kragujevac, Faculty of Science, Institute of Biology and Ecology, Kragujevac, Serbia

DOI: https://doi.org/10.2298/VSP170313118C

Ključne reči: dojka, neoplazme;, bolest, progresija; matriks, metaloproteinaze 9;, rizik, procena;, tkiva.

Sažetak

Uvod/Cilj. Karcinom dojke je jedna od najčešćih malignih bolesti žena širom sveta. Tumorsko mikrookruženje predstavlja jedan od glavnih regulatora tumorogeneze. Istraživali smo ulogu koncentracije matriks metaloproteinaze 9 (MMP-9) u peritumorskom tkivu kao prognostičkog markera kod bolesnica sa karcinomom dojke. Metode. ELISA test je korišćen za određivanje koncentracije ukupne MMP-9 u uzorcima karcinomskog i peritumorskog tkiva kod bolesnica sa karcinomom dojke. Vršeno je poređenje proteinske ekspresije MMP-9 sa kliničko-patološkim parametrima. Rezultati. Peritumorsko tkivo na distanci od 3 cm od tumora produkovalo je više MMP-9 nego sam tumor. Odnos koncentracija MMP-9 u tumorskom i peritumorskom tkivu se znatno menjalo u korist peritumorskog tkiva sa porastom veličine tumora i obimom zahvaćenosti aksilarnih limfnih nodusa. U N0 stadijumu odnos koncentracija MMP-9 u tumorskom i peritumorskom tkivu bio je 1 : 1,44, dok je u N2 stadijumu bolesti taj odnos bio čak 1 : 26,5. Zaključak. Kod bolesnica sa karcinomom dojke čak i u ranom stadijumu postoje promene u koncentraciji MMP-9 u peritumorskom tkivu koje mogu poslužiti kao prognostički parametar u proceni agresivnosti bolesti. Analizom odnosa MMP-9 u tumorskom i peritumorskom tkivu možemo izdvojiti grupu bolesnica koja je pod povećanim rizikom od razvoja limfonodalnih metastaza. Statistički značajna razlika između koncentracija MMP-9 u peritumorskom tkivu i tkivu karcinoma postoji samo u slučaju metastatske bolesti, ali ne u M0 stadijumu, ukazujući na potrebu za ranom detekcijom još uvek nedijagnostikovanih metastaza kod takvih bolesnica.

Biografije autora

Danijela M. Cvetković, Danijela Cvetković, MSci Laboratory for Cell and Molecular Biology Department for Biology and Ecology Faculty of Science, University of Kragujevac Radoja Domanovica 12, 34000 Kragujevac, Serbia

MSci

Bojan Z. Milošević, University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, Serbia

MD, MSci

Aleksandar M. Cvetković, University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, Serbia

MD, PhD

Srdjan M. Ninković, University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, Serbia

MD, PhD

Jovana V. Jovankić, University of Kragujevac, Faculty of Medical Sciences, Department of Surgery, Kragujevac, Serbia

Msci

Dalibor V. Jovanovic, University of Kragujevac, Faculty of Medical Sciences, Department of Pathology, Kragujevac, Serbia

Msci

Snežana D. Marković, University of Kragujevac, Faculty of Science, Institute of Biology and Ecology, Kragujevac, Serbia

Proffesor PhD

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Koncentracija matriks metaloproteinaze 9 u tumoru i peritumorskom tkivu kao prognostički marker kod bolesnica sa karcinomom dojke

Sažetak

Biografije autora

Reference