In vitro cytotoxicity assessment of the 3D printed polymer based epoxy resin intended for use in dentistry

Tatjana Puškar; Branka Trifković; Daniela Djurović Koprivica; Vesna Kojić; Ana Jevremović; Siniša Mirković; Dominic Eggbeer

doi:10.2298/VSP170721127P

Tatjana Puškar University of Novi Sad, Faculty of Medicine, Department of Dentistry, Novi Sad, Serbia
Branka Trifković University of Belgrade, Faculty of Dentistry, Clinic for Prosthodontics, Belgrade, Serbia
Daniela Djurović Koprivica University of Novi Sad, Faculty of Medicine, Department of Dentistry, Novi Sad, Serbia
Vesna Kojić University of Novi Sad, Faculty of Medicine, Oncology Institute of Vojvodina, Sremska Kamenica, Serbia
Ana Jevremović University Business Academy in Novi Sad, Faculty of Dentistry, Pančevo, Serbia
Siniša Mirković University of Novi Sad, Faculty of Medicine, Department of Dentistry, Novi Sad, Serbia
Dominic Eggbeer Cardiff Metropolitan University, National Centre for Product Design and Development Research, Cardiff, United Kingdom

DOI: https://doi.org/10.2298/VSP170721127P

Ključne reči: ćelije, kultura;, stomatološki materijali;, smole, sintetičke;, materijali, testiranje;, štampanje, trodimenzionalno.

Sažetak

Uvod/Cilj. Malo je objavljenih dokaza o citotoksičnosti 3D štampanih polimernih materijala za upotrebu u stomatologiji, bez obzira na njihovu sve širu primenu u medicini. Stereolitografija (SLA) jedan je od najvažnijih 3D procesa koji se primenjuje za 3D štampu, ali postoji samo mali broj materijala na bazi smola za koje je dokazano da su pogodni za medicinsku primenu. Cilj ove studije je bio da se ispita, in vitro, citotoksični efekat 3D štampanog polimera kako bi se utvrdila mogućnost za njegovu upotrebu u stomatologiji i srodnim medicinskim oblastima, kao što su hirurške dentalne vođice, okluzalni splintovi i ortodontski aparati. Metode. Da bi se ispitala citotoksičnost 3D štampanog polimera na bazi epoksi smole, Accura^® ClearVue ™ (3D-Sistems, USA), korišćene su dve ćelijske kulture: fibroblasti miša L929 i humani fibroplasti pluća MRC-5. Vijabilnost ćelija utvrđena je Mosmannovim kolorimetrijskim testom (MTT) i testom difuzije agara (ADT). Rezultati. Direktan kontakt testiranog materijala ispitan pomoću ADT pokazao je da materijal nije imao citotoksičan efekat ni na jednu ćelijsku kulturu. Testitrani materijal je imao blag citotoksični efekat posle 5, 7 i 21 dana ekstrakcije eluata primenom MTT na obe ćelijske linije. Citotoksičnost je rasla sa produženjem vremena ekstrakcije eluata. Zaključak. 3D štampani polimer na bazi epoksi smole, Accura^® ClearVue ™ (3D-Sistems, USA) se može smatrati pogodnim za izradu hirurških dentalnih implantnih vođica sa tačke gledišta njegovog citotoksičnog uticaja. Zbog pokazanih blagih citotoksičnih efekata nakon dužih ekstrakcionih perioda eluata potrebna su dalja istraživanja u oblasti biokompatibilnosti materijala da bi se taj polimer mogao koristiti za izradu okluzalnih splintova i ortodontskih aparata.

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In vitro procena citotoksičnosti 3D štampanog polimera na bazi epoksi smole namenjenog za upotrebu u stomatologiji

Sažetak

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