HIF-1α and SOX2 expression in cervical squamous cell carcinoma

Svetlana V. Milenković; Tatjana Terzić; Boris Vraneš; Ivan Soldatović

doi:10.2298/VSP171213043M

Svetlana V. Milenković Clinical Center of Serbia, Pathology Department, Belgrade, Serbia
Tatjana Terzić University of Belgrade, Faculty of Medicine, Institute of Pathology, Belgrade, Serbia
Boris Vraneš Clinical Center of Serbia, Clinic for Gynecology and Obstetrics, Belgrade, Serbia
Ivan Soldatović University of Belgrade, Faculty of Medicine, Institute for Medical Statistics and Informatics, Belgrade, Serbia

DOI: https://doi.org/10.2298/VSP171213043M

Ključne reči: materica, neoplazme, neoplazme, određivanje stadijuma, prognoza, hipoksija, biološki pokazatelji

Sažetak

Apstrakt

Uvod/Cilj. Hipoksija je jedna od bitnih promena koja se dešava u mikrookolini tumora. Zapaženo je da u populaciji kancerskih ćelija postoje pluripotentne kancerske stem ćelije koje utiču na rast tumora i njihovu rezistenciju na terapiju. Cilj rada je bio da se kod bolesnica sa skvamoznim karcinomom (squamous cell carcinoma – SCC) grlića materice ispita ekspresija faktora 1 alfa indukovanog hipoksijom (hypoxia-inducible factor-1 alpha – HIF-1α), endogenog markera hipoksije i SOX2, markera pluriponentnih stem ćelija koje postoje u normalnim tkivima odraslog čoveka. Metode. U ispitivanje je bilo uključeno 90 žena sa invazivnim SCC grlića materice podeljenih u dve grupe – 60 žena u grupi A, sa tumorima stadijuma FIGO IB1 < 20 mm (bez metastaza u limfnim nodusima) i 30 žena u grupi B, sa tumorima FIGO I–II (sa pozitivnim limfonodalnim statusom). Osnovni kliničkopatološki parametri su bili određeni standardnom patohistološkom analizom, a ekspresija HIF-1α i SOX2 imunohistohemijskim ispitivanjem. Rezultati. Između grupa A i B utvrđena je značajna razlika u ekspresiji HIF-1α (p = 0,024), ali ne i u ekspresiji SOX2 (p = 0,566). Ekspresija HIF-1α bila je značajno viša u grupi sa limfonodalnim metastazama i invazijom limfovaskularnih prostora (p < 0,001), ali nije bila povezana sa veličinom tumora (p = 0,291) niti jačinom limfocitnog odgovora (p = 0,940). Gradus tumora je značajno uticao na ekspresiju HIF-1α (p = 0,013). Ekspresija SOX2 nije značajno korelisala ni sa jednim utvrđenim kliničkopatološkim parametrom tumora. Zaključak. Značajna razlika u ekspresiji HIF-1α između grupa sa i bez metastaza u limfnim nodusima kod invazivnog SCC grlića materice mogla bi izdvojiti HIF-1α kao parametar loše prognoze bolesti. Nije utvrđen prognostički značaj SOX2, niti značajna uzajamna korelacija ekspresije HIF-1α i SOX2.

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Ekspresija HIF-1α i SOX2 kod skvamoznih karcinoma grlića materice

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