Production of immunoregulatory cytokines in clinically asymptomatic periapical lesions depends on the lesions size

Miloš Duka; Dragan Gazivoda; Milan Marković; Ivana Majstorović; Željka Tatomirović; Dušan Mihajlović; Miodrag Čolić

doi:10.2298/VSP170208036D

Miloš Duka Military Medical Academy, Clinic for Stomatology, Belgrade, Serbia; University of Defence in Belgrade, Faculty of Medicine of the Military Medical Academy, Belgrade
Dragan Gazivoda Military Medical Academy, Clinic for Stomatology, Belgrade, Serbia; University of Defence in Belgrade, Faculty of Medicine of the Military Medical Academy, Belgrade
Milan Marković University of Defence in Belgrade, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbi
Ivana Majstorović Military Medical Academy, Institute for Medical Research, Belgrade, Serbia
Željka Tatomirović Military Medical Academy, Institute for Pathology, Belgrade, Serbia; University of Defence in Belgrade, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia
Dušan Mihajlović Military Medical Academy, Institute for Medical Research, Belgrade, Serbia; University of Defence in Belgrade, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia
Miodrag Čolić University of East Sarajevo, Faculty of Medicine, Republic of Srpska, Foča, Bosnia and Herzegovina

DOI: https://doi.org/10.2298/VSP170208036D

Ključne reči: periapeksne bolesti, citokini, mikroorganizmi, imunski faktori, zapaljenje

Sažetak

Apstrakt

Uvod/Cilj. Razvoj periapeksnih lezija (PLs) prolazi kroz složenu interakciju između patogenih mikroorganizama iz kanala zuba i imunskih mehanizama domaćina. U ovim procesima proinflamacijski citokini stimulišu zapaljenske reakcije i destrukciju kostiju dok anti-inflamacijski citokini sa imunoregulacijskim svojstvima imaju suprotan efekat. Način kako je taj balans kontrolisan je i dalje predmet brojnih studija. Cilj ovog rada bio je da se ispita da li lokalna produkcija interleukina (IL)-10, IL-27 i transformišućeg faktora rasta beta (TGF-β) u humanim asimptomatskim PLs zavisi od njihove veličine i kako nivo ovih citokina korelira sa ćelijskim sastavom PLs. Metode. Istraživanje je sprovedeno na 30 PLs koje su ekstrahovane u Klinici za stomatologiju VMA. Lezije su podeljene na grupu malih lezija (n = 12) i grupu velikih lezija (n = 18). Inflamatorne ćelije izolovane iz lezija (PL-ICs) kultivisane su u toku 24 časa u medijumu za ćelijske kulture sa dodatkom forbol miristat acetata i Ca²⁺jonofora. Citospin preparati obojeni su pomoću imunocitohemijskih metoda korišćenjem monoklonskih antitela prema ćelijskim subpopulacijama. Nivo citokina u supernatantima ćelijskih kultura određen je ELISA metodom. Za statističku obradu podataka korišćen je Studentov t-test i Spearman-ov test korelacije. Vrednosti razlika p < 0,05 smatrane su statistički značajnim. Rezultati. Nivoi IL-10 i TGF-β su bili statistički značajno viši u supernatantima PL-ICs velikih lezija (p < 0,01, odnosno p < 0,05), za razliku od IL-27 čiji su nivoi bili veći u kulturama malih lezija (p < 0,05). Iako je ukupan broj PL-ICs i procenat mononuklearnih fagocita bio viši u velikim lezijama (p < 0,01, odnosno p < 0,05), njihov osnovni ćelijski sastav nije se bitnije razlikovao između grupa. Procenat B ćelija/plazma ćelija (CD19/CD38⁺ćelija), CD8⁺ T ćelija i CD14⁺ćelija je bio veći u velikim lezijama (p < 0,005; p < 0,05; p < 0,05), za razliku od procenta ukupnih CD3⁺T ćelija koji bio je veći u malim lezijama (p < 0,05). Nisu nađene korelacije između nivoa ispitivanih citokina i ćelijskog sastava/fenotipa PL-ICs. Zaključak. Ova studija ukazuje na to da IL-10, IL-27 i TGF-β najverovatnije imaju različitu ulogu u suzbijanju neželjenih imunskih/inflamacijskih reakcija u asimptomatskim PLs, zavisno od ekstezivnosti patološkog procesa, procenjivanog na osnovu veličine lezije.

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Zavisnost produkcije imunoregulatornih citokina u klinički asimptomatskim periapeksnim lezijama od veličine lezije

Sažetak

Reference