Postmortalne vrednosti serotonina u cerebrospinalnoj tečnosti kao marker porekla smrti

  • Irina Damjanjuk University of Belgrade, Faculty of Medicine, Institute of Forensic Medicine, Belgrade, Serbia
  • Vesna Popović University of Belgrade, Faculty of Medicine, Institute of Forensic Medicine, Belgrade, Serbia
  • Vera Lukić University of Belgrade, Faculty of Medicine, Institute of Forensic Medicine, Belgrade, Serbia
  • Ivan Soldatović University of Belgrade, Faculty of Medicine, Institute of Medical Statistics and Informatics, Belgrade, Serbia
  • Zoran Mihailović University of Belgrade, Faculty of Medicine, Institute of Forensic Medicine, Belgrade, Serbia
  • Tatjana Atanasijević University of Belgrade, Faculty of Medicine, Institute of Forensic Medicine, Belgrade, Serbia
DOI: https://doi.org/10.2298/VSP170221049D
Ključne reči: autopsija, biološki pokazatelji, smrt, uzrok, cerebrospinalna tečnost, hromatografija, tečna, smrt, medicina, sudska, serotonin

Sažetak


Uvod/Cilj. Serotonin [5-hidroksitriptamin (5-HT)], kao neu­rotransmiter u centralnom nervnom sistemu (CNS), uključen je u regulaciju autonomnih i kognitivnih funkcija, obrade sen­zornih informacija, motorne aktivnosti, emocija, raspoloženja i gotovo svakog oblika ponašanja. U sudskomedicinskoj is­trazi, 5-HT je proučavan u različitim telesnim tečnostima u odnosu na uzrok smrti, a posebno u vezi sa samoubistvima, zloupotrebom droga ili kao marker akutnog odgovora na stres. Cilj ove studije bio je utvrđivanje nivoa 5-HT u cere­brospinalnoj tečnosti, kao markera njegove centralne aktivno­sti tokom procesa umiranja, u smrtima različitog porekla, a posebno u slučajevima gde su žrtve bile svesne stresnog događaja. Metode. Istraživanje je sprovedeno na postmortal­nim uzorcima 81 likvora uzetih tokom obdukcije. Koncentra­cije 5-HT su određivane u odnosu na prirodno i nasilno (za­desno, samoubilačko i ubilačko) poreklo smrti. Nakon pri­preme, uzorci su analizirani metodom tečne hromatografije sa tandem masenom spektrometrijom. Rezultati. Vrednosti 5-HT su bile značajno više u slučajevima nasilnih smrti u od­nosu na prirodne smrti (U = 519,000; p < 0.05). Utvrđene su razlike u srednjim vrednostima 5-HT između različitih uzroka smrti (usled trovanja, povređivanja dejstvom tupine, šiljka i oštrice, a niže u hipotermiji i srčanoj smrti). Vrednosti 5-HT značajno su se razlikovale među grupama (χ2 = 13,354; p = 0.001, sa tendencijom sniženja sa godinama. Nisu utvrđene razlike u odnosu na pol, dužinu agonije i svesnost o nastupajućem smrtnom ishodu. Vrednosti su imale tendenciju rasta sa dužinom postmortalnog intervala, ali se to nije poka­zalo statistički značajnim. Zaključak. Serotonin bi mogao biti koristan postmortalni biohemijski marker u razlikovanju pri­rodnih i nasilnih smrti, uprkos velikim individualnim varijaci­jama.

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Objavljeno
2021/08/19
Broj časopisa
Vol. 77 Br. 1 (2020): Vojnosanitetski pregled
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Originalni članak