Primena kalcijum-hidroksiapatita i faktora rasta u endodontskoj terapiji
Sažetak
Uvod/Cilj. Hidroksiapatit (HAp) je jedna od najčešće korišćenih kalcijum fosfatnih biokeramika koja ispoljava osteokonduktivna svojstva. Faktori rasta direktno indukuju morfološku i funkcionalnu diferencijaciju neodontoblasta. Cilj rada je bio ispitivanje efikasnosti biomaterijala na bazi HAp u kombinaciji sa faktorom rasta-β1 (TGF-β1) u stvaranju novog dentina i zatvaranju apeksa kanala korena na zubima eksperimentalnog modela. Metode. Kao eksperimentalni animalni model korišćeni su zubi glodara (zečeva). Nakon uklanjanja pulpe zuba pulpekstirpatorom kod vitalne pulpektomije, u apeksnom delu na nivou fiziološkog apikalnog foramena, lentulo spiralom aplikovan je biomaterijal. Eksperiment je obavljen u opštoj anesteziji. Životinje su održavane u životu 3, 6 i 12 meseci posle tretmana. Ekstrahirani zubi su analizirani pomoću scanning elektronske mikroskopije. Rezultati. Scanning elektronskom mikroskopijom dokazano je da je broj zuba sa novostvorenim dentinom i apeksnom opturacijom kanala veći 12 meseci nakon tretmana. Zaključak. Utvrđeno je da je u eksperimentalnim grupama tretiranim biomaterijalom, sa ili bez TGF-β1, došlo do apeksne opturacije kanala korena zuba novostvorenim dentinom.
Reference
Teodorović N. Hydroxyapatite-based ceramic materials in endo-dontic therapy of dental root canals. Monograph. Belgrade: University of Belgrade, Faculty of Dentistry; 2004. (Serbian)
Tronstad L. Tissue reactions following apical plugging of the root canal with dentin chips in monkey teeth subjected to pulpectomy. Oral Surg Oral Med Oral Pathol 1978; 45(2): 297‒304.
Tеоdorović N. Research in adhesive performances of three ca-nals sealers – SEM stady. 7th Congress of the Balcan Stomato-logical Society (BaSS); 2002 March 28‒30; Kushadasi, Turkie 2002.
Petrović V. Modalities of use of hydroxyapatite in apexogenesis [dissertation]. Belgrade: University of Belgrade, Faculty of Dentistry; 2007. (Serbian)
Taha NA, Abdulkhader SZ. Full Pulpotomy with Biodentine in Symptomatic Young Permanent Teeth with Carious Expo-sure. J Endod 2018; 44(6): 932‒7.
Gollmer L. The Use of Dentin Debris as a Root Canal Filling. Int J Orthod 1937; 23: 101–2.
Dianat O, Mashhadi Abas F, Paymanpour P, Eghbal MJ, Had-dadpour S, Bahrololumi N. Endodontic repair in immature dogs’ teeth with apical periodontitis: blood clot vs plasma rich in growth factors scaffold. Dent Traumatol 2017; 33(2): 84‒90.
Jacobsen EL, Bery PF, BeGole EA. The effectiveness of apical dentin plugs in sealing endodontically treated teeth. J Endod 1985; 11(7): 289‒93.
Popović-Bajić M. Impact of amelogenin, growth factors and new nanostructural materials based on calcium silicate cements on pulp regeneration [dissertation]. Belgrade: University of Bel-grade, Faculty of Dentistry; 2015. (Serbian)
Besinis A, van Noort R, Martin N. Remineralization potential of fully demineralized dentin infiltrated with silica and hydroxy-apatite nanoparticles. Dent Mater 2014; 30(3): 249‒62.
Jalan AL, Warhadpande MM, Dakshindas DM. A comparison of human dental pulp response to calcium hydroxide and Bio-dentine as direct pulp-capping agents. J Conserv Dent 2017; 20(2): 129‒33.
Hegde S, Sowmya B, Mathew S, Bhandi SH, Nagaraja S, Dinesh K. Clinical evaluation of mineral trioxide aggregate and bio-dentine as direct pulp capping agents in carious teeth. J Con-serv Dent 2017; 20(2): 91‒5.
Li Z, Cao L, Fan M, Xu Q. Direct pulp capping with calcium hydroxide or mineral trioxide aggregate: A meta-analysis. J Endod 2015; 41(9): 1412‒7.
Bimstein E, Rotstein I. Cvek pulpotomy - revisited. Dent Traumatol 2016; 32(6): 438‒42.
Teodorovic, N. Use of ceramic biomaterials in dental root canal treatment canals [dissertation]. Belgrade: University of Bel-grade, Faculty of Dentistry; 1998. (Serbian)
Djordjevic M. Comparative study of hydroxyapatie and calcium hydroxyde as the materials for direct and indirect pulp capping [dissertation]. Belgrade: University of Belgrade, Faculty of Dentistry; 2004. (Serbian)
Melih I. Experimental and clinical evaluation of adhesion of different materials for the root canal obturation [dissertation]. Pančevo: Faculty of Dentistry, University of Economics Academy in Novi Sad; 2015. (Serbian)
Brizuela C, Ormeño A, Cabrera C, Cabezas R, Silva CI, Ramírez V, et al. Direct Pulp Capping with Calcium Hydroxide, Min-eral Trioxide Aggregate, and Biodentine in Permanent Young Teeth with Caries: A Randomized Clinical Trial. J Endod 2017; 43(11): 1776‒80.
Pissiotis E, Spangberg LS. Biological evaluation of collagen gels containing calcium hydroxide and hydroxyapatite. J Endod 1990; 16(10): 468‒73.
Grossman L. Short-cuts in endodontic practice: are they worth the risks? Oral Health 1976; 66(12): 9‒10.
Sugawara A, Chow LC, Takagi S, Chohayeb H. In vitro evalua-tion of the sealing ability of a calcium phosphate cement when used as a root canal sealer-filler. J Endod 1990; 16(4): 162‒5.
Mongiorgi R, Prati C, Bertocchi G, Monti S. Chemistry and struc-ture of a new canal sealer showing a dynamical behaviour. Boll Soc Ital Biol Sper 1993; 69(6): 415‒22.
Melin M, Joffre-Romeas A, Farges JC, Couble ML, Magloire H, Bleicher F. Effects of TGFbeta1 on dental pulp cells in cul-tured human tooth slices. J Dent Res 2000; 79(9): 1689‒96.
Santos SCNDS, Sigurjonsson ÓE, Custódio CA, Mano JFCDL. Blood Plasma Derivatives for Tissue Engineering and Regen-erative Medicine Therapies. Tissue Eng Part B Rev 2018; 24(6): 454‒62.
Verstraete FJM, Osofsky A. Dentistry in pet rabbits. Comp Cont Educ Pract 2005; 27(9): 671–84.
Tziafa C, Koliniotou-Koumpia E, Papadimitriou S, Tziafas D. Den-tinogenic responses after direct pulp capping of miniature swine teeth with Biodentine. J Endod 2014; 40(12): 1967‒71.
Orhan EO, Maden M, Senguüven B. Odontoblast-like cell num-bers and reparative dentine thickness after direct pulp capping with platelet-rich plasma and enamel matrix derivative: a his-tomorphometric evaluation. Int Endod J 2012; 45(4): 317‒25.
Laurent P, Camps J, About I. Biodentine(TM) induces TGF-β1 release from human pulp cells and early dental pulp minerali-zation. Int Endod J 2012; 45(5): 439‒48.
Girish K, Mandava J, Chandra RR, Ravikumar K, Anwarullah A, Athaluri M. Effect of obturating materials on fracture re-sistance of simulated immature teeth. J Conserv Dent 2017; 20(2): 115‒9.
Teodorović N, Martinović Ž. Significance of apico-coronal tech-nique of dental root canal preparation in endodontic therapy using hydroxyapatite-based sealants. Vojnosanit Pregl 2005; 62(6): 447‒52. (Serbian)
Zhang L, Li QL, Cao Y, Wang Y. Regenerating a monoblock to obturate root canalsvia a mineralising strategy. Sci Rep 2018; 8(1): 13356