Efekti hiperbarične oksigenacije na kardiodinamiku i oksidacioni stres kod pacova sa sepsom

  • Aleksandar Jevtić Institute for Orthopedic Surgery “Banjica”, Belgrade, Serbia
  • Vladimir Živković University of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia
  • Milica Milinković University of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia
  • Željko Mijailović University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, Serbia
  • Nevena Draginić University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, Serbia
  • Marijana Andjić University of Kragujevac, Faculty of Medical Sciences, Department of Pharmacy, Kragujevac, Serbia
  • Andjela Milojević Šamanović Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia
  • Sergey Bolevich 1st Moscow State Medical University IM Sechenov, Department of Human Pathology, Moscow, Russian Federation
  • Vladimir Jakovljević University of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia
DOI: https://doi.org/10.2298/VSP191026142J
Ključne reči: hiperbarička oksigenacija, stres, oksidativni, sepsa, srce, pacovi

Sažetak


Uvod/Cilj. Disfunkcija na nivou ćelije, tkiva ili organa, koja može imati za posledicu smrtni ishod, javlja se usled metaboličkih promena i poremećaja regulacije transkripcije gena i mikro- i makro cirkulacije. Cilj ove studije bio je da se proceni uticaj hiperbarične oksigenacije (HBO) na izolovano srce, kao i na oksidacioni status pacova sa sepsom. Metode. Istraživanjem su obuhvaćeni mužjaci Wistar albino pacova klasifikovani u tri grupe: prva grupa je bila kontrolna grupa (CTRL), drugu grupu su činile životinje izložene samo sepsi bez HBO tretmana (grupa Sepsa), dok su u trećoj grupi životinje tretirane HBO nakon indukcije sepse (grupa Sepsa + HBO). Za indukciju sepse korišćen je model fekalnog peritonitisa (3 mL/kg fekalne suspenzije, intraperitonealno). Posle indukcije sepse, pacovi su bili izloženi dva puta dnevno (tokom 12 sati) HBO tretmanu sa 2,8 apsolutnih atmosfera (ATA) tokom 90 minuta u periodu od 3 dana. 72 h nakon potvrđivanja sepse, životinje su žrtvovane, a srca su retrogradno perfundovana na Langendorfovom aparatu, pri postepenom povećanju koronarnog perfuzionog pritiska (CPP = 40–120 cm H2O). Sledeći parametri srčane funkcije su kontinuirano mereni: maksimalna i minimalna stopa promene pritiska u levoj komori (dp/dt max, dp/dt min); sistolni i dijastolni pritisak leve komore (SLVP i DLVP) i srčana frekvenca (HR). Koronarni protok (CF) je meren floumetrijski. Određivani su sledeći markeri oksidacionog stresa: nitriti (NO2−), superoksid anjon radikal (O2−), vodonik peroksid (H2O2), indeks lipidne peroksidacije (TBARS), aktivnost superoksid dismutaze (SOD) i katalaze (CAT) i nivo redukovanog glutationa (GSH). Rezultati. Nije bilo značajne razlike u dp/dt max, dp/dt min, SLVP i HR između grupa. CF je bio statistički značajno veći (p < 0,01) u grupi sa sepsom. Vrednosti svih srčanih oksidacionih markera bile su niže u grupi sepsa + HBO (p < 0,05), dok su sistemski pro-oksidacioni i antioksidacioni parametri bili nepromenjeni. Zaključak. Naši rezultati su pokazali da HBO tretman nije bio povezan sa poboljšanom funkcijom srca i koronarnom perfuzijom, dok je ostvario obećavajući korisne efekte na oksidacioni status u srcu pacova.

Biografija autora

Vladimir Živković, University of Kragujevac, Faculty of Medical Sciences, Department of Physiology, Kragujevac, Serbia

 

 

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Objavljeno
2021/08/24
Broj časopisa
Vol. 78 Br. 8 (2021): Vojnosanitetski pregled
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Originalni članak