Prednosti i nedostaci biodentina: zadovoljavajuća mehanička svojstva i radiološka vidljivost koja ne zadovoljava ISO standard
Sažetak
Uvod/Cilj. U stomatologiji, koncept primene inertnih materijala u cilju reparacije tkiva zamenjen je strategijom pronalaženja bioaktivnih materijala koji pozitvno deluju na humana tkiva. Cilj studije bio je karakterizacija fizičko-hemijskih osobina komercijalno dostupnog dentalnog cementa na bazi kalcijum silikata i kalcijum karbonata, biodentina (Septodont, Francuska). Metode. Ispitivanje materijala obuhvatilo je merenja radiografskog kontrasta, skening elektronsku mikroskopiju i radiografsku disperzivnu analizu, kvašenje, Furier-ovu transformocionu infracrvenu spektroskopiju, čvrstinu, mikro- i nano-poroznost, vremena vezivanja, pH vrednosti i oslobađanja kalcijumovih jona. Ćelije (mezenhimalne stem ćelije kostne srži miša – BMSCs) su uzgajane na površini biodentina sa ciljem da se ispita njegovo ponašanje u biološkim uslovima. Rezultati. Radiološki kontrast cementa (2.8 mm Al) bio je niži od ISO standarda za materijale namenjene punjenju kanala korena. Cement se sastojao od finog praha sa česticama male veličine i oblika koji se iz ovalnog menjao u kockasti nakon potapanja u simuliranu telesnu tečnost. Biodentin je pokazao dobre mikromehaničke osobine i nisku poroznost sa prosečnom veličinom pora 92 μm. Kvašenje (Ca = 41°), oslobađanje jona kalcijuma (0,098 μg/cm²) i pH rastvora gde je čuvan (9.07), pokazali su zadovoljavajuće karakteristike. BMSCs u neposrednom kontaktu sa cementnim česticama zadržale su vijabilnost što je ukazalo na dobru biokompatibilnost biodentina. Zaključak. Biodentin je ispoljio dobre mehaničke i fizičko-hemijske karakteristike, ali bez zadovoljavajućeg radiografskog kontrasta.
Reference
Jokanović V, Čolović B, Marković D, Petrović M, Soldatović I, Anto-nijević D, et al. Extraordinary biological properties of a new calcium hydroxyapatite/poly(lactide-co-glycolide)-based scaf-fold confirmed by in vivo investigation. Biomed Tech (Berl) 2017; 62(3): 295−306.
Ćetenović B, Čolović B, Vasilijić S, Prokić B, Pašalić S, Jokanović V, et al. Nanostructured endodontic materials mixed with differ-ent radiocontrast agents-biocompatib. J Mater Sci Mater Med 2018; 29(12): 190.
Li Q, Hurt AP, Coleman NJ. The Application of 29Si NMR Spectroscopy to the Analysis of Calcium Silicate-Based Ce-ment using Biodentine as an Example. J Funct Biomater 2019; 10(2): pii: E25.
Weber MT, Hannig M, Pötschke S, Höhne F, Hannig C. Applica-tion of plant extracts for the prevention of dental erosion: an in situ/in vitro study. Car Res 2015; 49(5): 477–87.
Mohamed DA, Fayyad DM. The effect of different bioactive materials on the odontogenic differentiation potential of den-tal pulp stem cells using two different culture mediums. Tanta Dent J 2017; 14(3): 120–8.
Kamal EM, Nabih SM, Obeid RF, Abdelhameed MA. The repar-ative capacity of different bioactive dental materials for direct pulp capping. Dent Med Probl 2018; 55(2): 147−52.
Cetenovic B, Prokic B, Vasilijic S, Dojcinovic B, Magic M, Jokanovic V, et al. Biocompatibility Investigation of New Endodontic Materials Based on Nanosynthesized Calcium Silicates Com-bined with Different Radiopacifiers. J Endod 2017; 43(3): 425−32.
Medigovic I, Antonijevic D. In vitro radiographic density of den-tal posts measured by digital radiography. Oral Radiol 2014; 30: 9–12.
Careddu R, Duncan HF. How does the pulpal response to Bio-dentine and ProRoot mineral trioxide aggregate compare in the laboratory and clinic? Br Dent J 2018; 225: 743−9.
Antonijevic D, Medigovic I, Zrilic M,Jokic B, Vukovic Z, Todorovic Lj. The influence of different radiopacifying agents on the ra-diopacity, compressive strength, setting time, and porosity of Portland cement. Clin Oral Invest 2014; 18(6): 1597–604.
Ilić D, Antonijević Dj, Biočanin V, Čolović B, Danilović V, Komlev V, et al. Physico-chemical and biological properties of dental calcium silicate cements - literature review. Hemijska industri-ja 2019; 73(5): 281−94.
Liedke GS, Spin-Neto R, da Silveira HE, Wenzel A. Radiograph-ic diagnosis of dental restoration misfit: a systematic review. J Oral Rehabil 2014; 41(12): 957–67.
Tuğ Kılkış B, Er K, Taşdemir T, Yildirim M, Taskesen F, Tü-mkaya L, et al. Neurotoxicity of various root canal sealers on rat sciatic nerve: an electrophysiologic and histopathologic study. Clin Oral Investig 2015; 19(8): 2091–100.
Liedke GS, Spin-Neto R, Vizzotto MB, Da Silveira PF, Silveira HE, Wenzel A. Diagnostic accuracy of conventional and digi-tal radiography for detecting misfit between the tooth and res-toration in metal-restored teeth. J Prosth Dent 2015; 113(1): 39–47.
Goracci C, Juloski J, Schiavetti R, Mainieri P, Giovannetti A, Vichi A, Ferrari M. The influence of cement filler load on the radio-pacity of various fibre posts ex vivo. Int Endod J 2015; 48(1): 60−7.
Ochoa-Rodríguez VM, Tanomaru-Filho M, Rodrigues EM, Guerreiro-Tanomaru JM, Spin-Neto R, Faria G. Addition of zirconium oxide to Biodentine increases radiopacity and does not alter its physicochemical and biological properties. J Appl Oral Sci 2019; 27: e20180429.
Asgary S, Motazedian HR, Parirokh M, Eghbal MJ, Kheirieh S. Twenty years of research on mineral trioxide aggregate: A sci-entometric report. Iran Endod J 2013; 8(1): 1–5.
Choi Y, Park SJ, Lee SH, Hwang YC, Yu MK, Min KS. Biologi-cal effects and washout resistance of a newly developed fast-setting pozzolan cement. J Endod 2013; 39(4): 467–72.
Gandolfi MG, Taddei P, Siboni F, Modena E, Ginebra MP, Prati P. Fluoride-containing nanoporous calcium-silicate MTA ce-ments for endodontics and oral surgery: Early fluorapatite formation in a phosphate-containing solution. Int Endod J 2011; 44(10): 938–49.
Marciano MA, Duarte MAH, Camilleri J. Dental discoloration caused by bismuth oxide in MTA in the presence of sodium hypochlorite. Clin Oral Invest 2015; 19(9): 2201–9.
Kaur M, Singh H, Dhillon JS, Batra M, Saini M. MTA versus Bi-odentine: Review of literature with a comparative analysis. J Clin Diagn Res 2017; 11(8): ZG01−5.
Sauia TS, Gomes BP, Pinheiro ET, Zaia AA, Ferraz CC, Souza-Filho JF. Microleakage evaluation of intraorifice sealing mate-rials in endodontically treated teeth. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006; 102(2): 242−6.
Camilleri J. Characterization and hydration kinetics of tricalci-um silicate cement for use as a dental biomaterial. Dent Mater 2011; 27(8): 836–44.
De Souza ET, Nunes Tameirão MD, Roter JM, De Assis JT, De Almeida Neves A, De-Deus GA. Tridimensional quantitative porosity characterization of three set calcium silicate-based repair cements for endodontic use. Micro Res Tech 2013; 76(10): 1093–8.
Popović BM, Prokić B, Prokić BB, Jokanović V, Danilović V, Živ-ković S. Histological evaluation of direct pulp capping with novel nanostructural materials based on active silicate cements and biodentine on pulp tissue. Acta Vet (Beograd) 2013; 63: 347–60.
Grazziotin-Soares R, Nekoofar MH, Davies TE, Bafail A, Alhad-dar E, Hübler R et al. Effect of bismuth oxide on white miner-al trioxide aggregate: Chemical characterization and physical properties. Int Endod J 2014; 47(6): 520–33.
Elnaghy AM. Influence of acidic environment on properties of Biodentine and white mineral trioxide aggregate: A compara-tive study. J Endod 2014; 40(7): 953–7.
Chang SW, Lee SY, Ann HJ, Kum KY, Kim EC. Effects of cal-cium silicate endodontic cements on biocompatibility and mineralization-inducing potentials in human dental pulp cells. J Endod 2014; 40(8): 1194–200.
Li M, Guo D, Ma T, Zhang G, Shi Y, Zhang X. High fracture toughness in a hierarchical nanostructured zirconium. Mat Sci Eng A-Struct 2014; 606: 330–3.
Bosso-Martelo R, Guerreiro-Tanomaru JM, Viapiana R, Berbert FL, Duarte MA, Tanomaru-Filho M. Physicochemical properties of calcium silicate cements associated with microparticulate and nanoparticulate radiopacifiers. Clin Oral Invest 2016; 20(1): 83−90.
Rakocevic Z. Osnovi radiologije dento-maksilofacijalne regije. Principi i tehnike. Belgrade: Balkanski stomatološki forum; 1998. (Serbian)
Camilleri J, Sorrentino F, Damidot D. Investigation of the hydra-tion and bioactivity of radiopacified tricalcium silicate ce-ment, Biodentine and MTA Angelus. Dent Mater 2013; 29(5): 580–93.
Tanalp J, Karapınar-Kazandağ M, Dölekoğlu S, Baybora Kayahan M. Comparison of the radiopacities of different root-end fill-ing and repair materials. ScientificWorldJournal. 2013; 2013: 594950.
Kaup M, Schäfer E, Dammaschke T. An in vitro study of differ-ent material properties of Biodentine compared to ProRoot MTA. Head Face Med 2015; 11: 16.
Farrugia C, Lung CYK, Schembri Wismayer P, Arias-Moliz MT, Camilleri J. The Relationship of Surface Characteristics and Antimicrobial Performance of Pulp Capping Materials. J En-dod 2018; 44(7): 1115−20.
International Organization for Standardization. ISO 6876, Dental root canal sealing materials. 2nd ed. Geneva: 36. In-ternational Organization for Standardization; 2001.
Saghiri MA, Gutmann JL, Orangi J, Asatourian A, Sheibani N. Radiopacifier particle size impacts the physical properties of tricalcium silicate-based cements. J Endod 2015; 41(2): 225–30.
Grech L, Mallia B, Camilleri J. Investigation of the physical properties of tricalcium silicate cement-based root-end filling materials. Dent Mat 2013; 29(2): e20–8.
Kisić D, Nenadović M, Štrbac S, Adnadjević B, Rakočević Z. Effect of UV/ozone treatment on the nanoscale surface properties of gold implanted polyethylene. Appl Surf Sci 2014; 307: 311–8.
Kumari S, Mittal A, Dadu S, Dhaundiyal A, Abraham A, Yendrembam B. Comparative evaluation of physical and chem-ical properties of calcium silicate-based root-end filling mate-rials (mineral trioxide aggregate and biodentine): An in vitro study. Indian J Dent Sci 2018; 10(4): 197−202.
Laurent P, Camps J, About I. Biodentine(TM) induces TGF-β1 release from human pulp cells and early dental pulp minerali-zation. Int Endod J 2012; 45(5): 439−48.
Zou Q, Li Y, Zhang L, Zuo Y, Li J, Li X. Characterization and cytocompatibility of nano-hydroxyapatite/chitosan bone ce-ment with the addition of calcium salts. J Biomed Mater Res B Appl Biomater 2009; 90(1): 156−64.
Rasimick BJ, Shah RP, Musikant BL, Deutsch AS. Radiopacity of endodontic materials on film and a digital sensor. J Endod 2007; 33(9): 1098−101.