Uticaj različitih materijala koji oblažu hidroksiapatit koštanog nosača na uspeh regeneracije koštanih defekta kalvarije zeca: histomorfometrijska i histološka analiza

  • Sanja Milutinović-Smiljanić University of Belgrade, Faculty of Dental Medicine, Department of General and Oral Histology and Embryology, Belgrade, Serbia
  • Djordje Antonijević University of Belgrade, Institute for Nuclear Science “Vinča”, Laboratory for Atomic Physics, Belgrade, Serbia
  • Milutin Mićić University of Belgrade, Faculty of Medicine, Laboratory for Anthropology, Belgrade, Serbia
  • Vladimir Biočanin Stomatološki fakultet, Poslovna akademija, Pančevo, Srbija
  • Nikola Sjerobabin Klinički centar "Dragiša Mišović", Univerzitet u Beogradu, Srbija
  • Božana Petrović University of Belgrade, Institute for Nuclear Science “Vinča”, Laboratory for Atomic Physics, Belgrade, Serbia
  • Vesna Danilović University of Belgrade, Faculty of Dental Medicine, Department of General and Oral Histology and Embryology, Belgrade, Serbia
  • Vukoman Jokanović University of Belgrade, Institute for Nuclear Science “Vinča”, Laboratory for Atomic Physics, Belgrade, Serbia
DOI: https://doi.org/10.2298/VSP210513072M
Ključne reči: kost, regeneracija;, histološke tehnike;, materijali, testiranje;, polimeri;, zečevi.

Sažetak


Uvod/Cilj. Materijali koji se u današnje vreme koriste za nadoknadu koštanog tkiva ne dovode do kompletne regeneracije, zbog čega se ispituju novi. Uprkos mnogobrojnim pokušajima da se poboljša regeneracija koštanog tkiva, još uvek nije pronađen materijal koji ispunjava sve kriterijume. Cilj rada bio je da se utvrdi uticaj poli(laktid-ko-glikolida) (PLGA) i polietilenimina (PEI), kao premaza za oblaganje hidroksiapatita (HAP), na regenerativni potencijal koštanog tkiva u defektu kalvarije zeca. Metode. Kod 19 zečeva „zrelog” skeleta načinjeni su defekti kalvarije dijametra 6 mm. Defekti su potom ispunjeni jednim od sledećih materijala: HAP obložen PLGA (HAP + PLGA), HAP obložen PEI (HAP + PEI) i goveđi HAP – Bio-Oss® (pozitivna kontrola). Prazni defekti su predstavljali negativnu kontrolu. Inflamacijska reakcija tkiva domaćina je ispitana histološkom analizom. Formiranje nove kosti je procenjivano histomorfometrijskom analizom. Analizirani su uzorci dobijeni 3, 6 i 9 nedelja nakon implantacije. Rezultati. Tri nedelje nakon implantacije, uočena je tendencija boljeg zarastanja u HAP + PLGA grupi, bez statistički značajne razlike između ispitivanih grupa (p > 0.05). Međutim, 6 i 9 nedelja nakon implantacije, primećeno je značajno formiranje koštanog tkiva u korist HAP + PLGA grupe (p < 0,05). Oblaganje HAP sa PLGA dovelo je do boljeg koštanog zarastanja u poređenju sa HAP+PEI i Bio-Oss®. Zaključak. U pogledu stimulisanja osteogeneze u rekonstruktivnoj hirurgiji kostiju, PLGA je pokazao veći potencijal prekrivanja defekta od PEI.

Biografija autora

Sanja Milutinović-Smiljanić, University of Belgrade, Faculty of Dental Medicine, Department of General and Oral Histology and Embryology, Belgrade, Serbia

dr medicinskih nauka, docent

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Objavljeno
2022/12/02
Broj časopisa
Vol. 79 Br. 10 (2022): Vojnosanitetski pregled
Rubrika
Originalni članak