Uporedna histohemijska i morfometrijska analiza mišićnih vlakana psoasnog mišića kod osoba oba pola tokom starenja
Sažetak
Uvod/Cilj. Veličina psoasnog mišića smanjuje se sa starenjem i histopatološka analiza (HPA) postmortalnog materijala takođe pokazuje značajne promene u njemu u zavisnosti od godina života. Cilj istraživanja bio je da se utvrdi prisustvo i distribucija mišićnih vlakana tipa I i II psoasnog mišića kod osoba različitih starosnih kategorija i različitog pola. Metode. Materijal su činili tkivni uzorci desnog bedrenoslabinskog mišića 30 odraslih kadavera (18 muških i 12 ženskih), starosti od 30 do 90 godina, raspoređenih u tri starosne grupe. Materijal je dobijen sa Instituta za sudsku medicinu, Medicinskog fakulteta Univerziteta u Nišu, Srbija. Hematoksilin-eozin (H&E) bojenje je korišćeno u HPA mišićnih ćelija. Rezultati. Vrednosti astereoloških parametara (area, perimetar i Feretov dijametar) mišićnih vlakana tipa I i tipa II bile su veće kod kadavera muškog pola u poređenju sa kadaverima ženskog pola, ali bez statistički značajne razlike. Na osnovu histohemijske i morfometrijske analize zaključeno je da nakon navršene 70. godine života dolazi do gubitka mišićnih vlakana tipa II, što je kod kadavera ženskog pola bilo izraženije. Zaključak. Sa starenjem, gubitak mišićnih vlakana tipa II, kao i atrofija mišićnih vlakana tipa I i tipa II pokazuju sličnu dinamiku kod oba pola.
Reference
Petrosyan K, Tamayo R, Joseph D. Sensitivity of a novel biotin-free detection reagent (Powervision+y) for immunohisto-chemistry. J Histotechnol 2002; 25(4): 247‒50.
Rosenberg IH. Sarcopenia: origins and clinical relevance. J Nutr 1997; 127(5Suppl): 990S‒991S.
Imamura K, Aschida H, Ischikawa T, Fuji M. Human major pso-as muscle and sacrospinalis muscle in relation to age: a study by computed tomografy. J Gerontol 1983; 38(6): 678‒81.
Shimokawa I, Yu BP, Higami Y, Ikeda T. Morphometric analysis of somatotrophs: effects of age and dietary restriction. Neuro-biol Aging 1996; 17(1): 79‒86
Hasegawa M. On the myofibrous organization of human iliop-soas muscle (psoas major muscle and ilacus muscle). J Showa Med Assoc 1987; 47(6): 833‒42.
Brown M. Change in fiber size, not number, in ageing sceletal muscle. Age Ageing 1987; 16(4): 244‒8.
Junqueira LC, Carneiro J. Basic histology – text and atlas. 11th ed. New York, NY: McGraw-Hill; 2005.
Michael R, Gordon K, Wojciech P. Histology: a text and atlas. 4th ed. Philadelphyia, Pennsylvania, USA: Lippincott Williams and Wilkins; 2002. p. 646‒50.
Dubowitz V, Sewrey CA. Muscle Biopsy. A Modern Approach. Philadelphia, PA; Saunders Elsevier; 2007.
Brooke MH, Kaiser KK. Muscle fiber types: how many and what kind? Arch Neurol 1970; 23(4): 369‒79.
Lexell J, Taylor CC, Sjöström M. What is the cause of the ageing atrophy? Total number, size and proportion of different fiber types studied in whole vastus lateralis muscle from 15- to 83-year-old men. J Neurol Sci 1988; 84(2‒3): 275‒94.
Kobayashi K. Myofibrous organization in human quadriceps femoris muscle. J Showa Med Assoc 1991; 51(2): 186‒96.
Roth SM, Ivey FM, Martel GF, Lemmer JT, Hurlbut DE, Siegel EL, et al. Muscle size responses to strenght training in young and older men and women. J Am Geriatr Soc 2001; 49(11): 1428‒ 33.
Solomon AM, Bouloux PM. Modifying muscle mass - the endo-crine perspective. J Endocrinol 2006; 191(2): 349‒60.
Horstman AM, Dillon EL, Urban RJ, Sheffield-Moore M. The role of androgens and estrogens on healthy aging of longevity. J Gerontol A Biol Sci Med Sci 2012; 67(11): 1140‒52.
Bennigton JL, Krupp M. Morfometric analysis of muscle. In: Heffner RR, editor. Muscle pathology. New York: Churchill Livngstone; 1984: p. 43‒71.
Lemmer JT, Hurlbut DE, Martel GH, Tracy BL, Ivey FM, Metter EJ, et al. Age and gender responses to strenght training and detraing. Med Sci Sports Exerc 2000; 32(8): 1505‒12.
Koopman R. Dietary protein and exercise training in ageing. Proc Nutr Soc 2011; 70(1): 104‒13.
Borst SE. Interventions for sarcopenia and muscle weakness in older people. Age Ageing 2004; 33(6): 548‒55.
Nilwik R, Snijders T, Leenders M, Groen BB, van Kranenburg J, Verdijk LB, et al. The decline in skeletal muscle mass with ag-ing is mainly attributed to a reduction in type II muscle fiber size. Exp Gerontol 2013; 48(5): 492‒8.
Arbanas J, Klasan GS, Nikolić M, Jerković R, Miljanović I, Malnar D. Fibre type composition of the human psoas major muscle with regard to the level of its origin. J Anat 2009; 215(6): 636‒41.
Arbanas J, Klasan GS, Nikolić M, Cvijanović O, Malnar D. Im-munohistochemical analysis of the human psoas major muscle with regards to the body side and aging. Coll Antropol 2010; 34(Suppl 2): 169‒73.
Volpi E, Nazemi R, Fujita S. Muscle tissue changes with aging. Curr Opin Clin Nutr Metab Care 2004; 7(4): 405‒10.
Rolland Y, Czerwinski S, Abellan Van Kan G, Morley JE, Cesari M, et al. Sarcopenia: its assessment, etiology, pathogenesis, consequences and future perspectives. J Nutr Health Aging 2008; 12(7): 433‒50.
Drey M. Sarcopenia - pathophysiology and clinical relevance. Wien Med Wochenchr 2011; 161(17‒18): 402‒8.
Antić VM, Stefanović N, Jovanović I, Antić M, Milić M, Krstić M, et al. Morphometric analysis of somatotropic cells of the ade-nohypophysis and muscle fibers of the psoas muscle in the process of aging in humans. Ann Anat 2015; 200: 44‒53.
Baumgartner RN, Waters DL, Gallagher D, Morley JE, Garry PJ. Predictors of skeletal muscle mass in elderly men and women. Mech Ageing Dev 1999; 107(2): 123‒36.