Altered high-density lipoprotein particle structure and antioxidant capacity in preeclampsia

Gorica Banjac; Daniela Ardalić; Petar Cabunac; Jasmina Ivanišević; Jelena Vekić; Željko Miković; Aleksandra Stefanović

doi:10.2298/VSP210413010B

Gorica Banjac Gynecology and Obstetrics Clinic “Narodni Front”, Belgrade, Serbia
Daniela Ardalić Gynecology and Obstetrics Clinic “Narodni Front”, Belgrade, Serbia
Petar Cabunac Gynecology and Obstetrics Clinic “Narodni Front”, Belgrade, Serbia
Jasmina Ivanišević University of Belgrade, Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade, Serbia
Jelena Vekić University of Belgrade, Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade, Serbia
Željko Miković Gynecology and Obstetrics Clinic “Narodni Front”, Belgrade, Serbia
Aleksandra Stefanović University of Belgrade, Faculty of Pharmacy, Department of Medical Biochemistry, Belgrade, Serbia

DOI: https://doi.org/10.2298/VSP210413010B

Ključne reči: antioksidansi, lipoproteini hdl, preeklampsija, trudnoća, komplikacije

Sažetak

Uvod/Cilj. Jedna od komplikacija koja se može javiti tokom trudnoće je razvoj preeklampsije (PE). Glavne karakteristike ovog stanja su visok krvni pritisak i vrlo često znaci oštećenja bubrega ili drugih organa. Ovo stanje pogađa 5–7% svih trudnica i jedan je od glavnih faktora morbiditeta i mortaliteta trudnica i fetusa ili novorođenčadi. Cilj ovog rada bio je ispitivanje strukturnih i funkcionalnih modifikacija lipoproteinskih čestica visoke gustine (high-density lipoprotein – HDL) u trudnoćama sa visokim rizikom (TVR) za razvoj PE. Metode. U longitudinalnoj prospektivnoj studiji učestvovala je ukupno 91 trudnica sa TVR od razvoja PE. Od ukupnog broja trudnica, kod njih 71, PE se nije razvila do kraja trudnoće i ova grupa je označena kao grupa bez PE (BPE). Kod preostalih 20 žena sa TVR se razvila PE do porođaja, i one su svrstane u grupu PE. Krv je uzimana za analizu na kraju svakog trimestra i pred porođaj. Raspodela HDL čestica je određivana metodom vertikalne elektroforeze u 3–31% gradijentu poliakrilamidnog gela. Antioksidativni kapacitet HDL čestica je određivan na osnovu aktivnosti enzima paraoksonaze 1 (PON1) vezanog za HDL. Aktivnost PON1 u serumu je određivana metodom kinetičke spektrofotometrije. Rezultati. Rezultati su pokazali da se udeo HDL_2b čestica značajno povećao u drugom trimestru (p < 0,05) i ostao je povećan do kraja trudnoće u grupi BPE. Aktivnost PON1 bila je značajno veća u trećem trimestru (p < 0,05) kod ove grupe trudnica. Udeo HDL_3a čestica se značajno smanjio u drugom trimestru u PE grupi trudnica (p < 0,05) i ostao je snižen do kraja trudnoće. Aktivnost PON1 enzima u PE gripi se nije menjala u toku trudnoće. Zaključak. Dislipidemija u trudnoći može biti posledica različitih modifikacija HDL čestica. Strukturne i funkcionalne modifikacije HDL čestica, kao jedan od adaptivnih mehanizama, kod trudnica kod kojih se razvila PE, nisu adekvatne i kao takve gube svoju ateroprotektivnu ulogu.

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Izmenjena struktura i antioksidativni kapacitet lipoproteinskih čestica visoke gustine u preeklampsiji

Sažetak

Reference