Expression of Kv4.2 and Kv4.3 potassium channels in human umbilical veins from normal, diabetic, and hypertensive pregnancies

Vladimir Djokić; Miloć Gostimirović; Jovana Rajković; Jelena Rakočević; Milica Labudović-Borović; Svetlana Janković; Jelena Stanišić; Milan Kostić; Miloš Djurić; Ljiljana Gojković-Bukarica

doi:10.2298/VSP211014005D

Vladimir Djokić University of Belgrade, Faculty of Medicine, Institute of Pharmacology, Clinical Pharmacology and Toxicology
Miloć Gostimirović University of Belgrade, Faculty of Medicine, Institute of Pharmacology, Clinical Pharmacology and Toxicology
Jovana Rajković University of Belgrade, Faculty of Medicine, Institute of Pharmacology, Clinical Pharmacology and Toxicology
Jelena Rakočević University of Belgrade, Faculty of Medicine, Institute of Histology and Embryology “Aleksandar Dj. Kostić”
Milica Labudović-Borović University of Belgrade, Faculty of Medicine, Institute of Histology and Embryology “Aleksandar Dj. Kostić”
Svetlana Janković University of Belgrade, Faculty of Medicine, Department of Obstetrics and Gynecology “Narodni front”
Jelena Stanišić Vinča Institute of Nuclear Sciences, Belgrade, Serbia
Milan Kostić Institut za nuklearne nauke Vinča, Beograd, Srbija
Miloš Djurić University of Belgrade, Faculty of Medicine, Institute of Pharmacology, Clinical Pharmacology and Toxicology
Ljiljana Gojković-Bukarica University of Belgrade, Faculty of Medicine, Institute of Pharmacology, Clinical Pharmacology and Toxicology

DOI: https://doi.org/10.2298/VSP211014005D

Ključne reči: dijabetes melitus;, ljudi;, hipertenzija;, trudnoća;, v.umbilicalis

Sažetak

Uvod/Cilj. Značajan niz dokaza ukazuje na to da su kanali Kv4.2 i Kv4.3 glavne komponente brzih prolazno-ispravljačkih struja kalijuma (struje tipa A). Pretpostavlja se da bi te struje mogle da budu uključene u održavanje membranskog potencijala ćelije, kao i u regulaciju propagacije i učestalosti akcionih potencijala. Međutim, vrlo malo se zna o prisustvu i funkciji struje tipa A u glatkim mišićima krvnih sudova čoveka, kao što je humana umbilikalna vena (HUV). S obzirom na njenu ključnu ulogu u adekvatnoj oksigenaciji fetusa, cilj rada bio je da se utvrdi da li su podtipovi kalijumovih kanala Kv4.2 i Kv4.3 prisutni u glatkim mišićima HUV-a i da se istraže potencijalne promene njihove ekspresije tokom patoloških stanja majke – gestacijskog dijabetesa melitusa (GDM) i arterijske hipertenzije (AH) izazvane trudnoćom (AHT). Metode. U istraživanje su uključene HUV sakupljene posle trudnoće zdravih porodilja, onih čije trudnoće su bile komplikovane GDM i AH. Svaka grupa sastojala se od 6 uzoraka HUV-a dobijenih iz 6 zdravih trudnoća, 6 trudnoća komplikovanih GDM i 6 trudnoća komplikovanih AH. Nakon farmakoloških analiza, urađene su imunohistohemijska (IH) analiza i Western blot. Rezultati. Primenom IH analize pokazan je sličan obrazac ekspresije obe podjedinice Kv4.2 i Kv4.3 kanala u glatkim mišićima HUV-a u svim grupama trudnica. Rezultati dobijeni Western blot-om bili su u skladu sa IH bojenjem. Ekspresija podjedinica Kv4.2 i Kv4.3 nije se značajno razlikovala između grupa trudnica. Zaključak. Ovo je prema našim saznanjima prva studija kojom je pokazano prisustvo Kv4.2 i Kv4.3 kalijumovih kanala u glatkim mišićima HUV-a zdravih porodilja, kao i onih porodilja čija je trudnoća bila praćena prisustvom GDM ili AHT. Ti kanali su najverovatnije glavne komponente brzih struja A-tipa, koji mogu biti relevantni za protok krvi majke i ploda i na taj način delovati protektivno za razvoj fetusa. Takođe, oni mogu predstavljati senzitivne pokazatelje hemodinamskih i/ili metaboličkih promena u lokalnoj fetomaternalnoj sredini.

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Ekspresija kalijumovih kanala Kv4.2 i Kv4.3 u humanim pupčanim venama zdravih trudnica, trudnica sa gestacijskim dijabetesom melitusom i trudnica sa gestacijskom hipertenzijom

Sažetak

Reference