Morfometrijska analiza kolagena i inflamatornih ćelija u periodontalnoj bolesti

  • Ranko Golijanin Department of Dentistry, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia; Faculty of Dental Medicine, University of East Sarajevo, Foča, Republic of Srpska, Bosnia and Herzegovina
  • Bojan Kujundžić Faculty of Dental Medicine, University of East Sarajevo, Foča, Republic of Srpska, Bosnia and Herzegovina
  • Zoran Milosavljević Department of Histology and Embriology, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia
  • Dragan R Milovanović Department of Pharmacology and Toxicology,Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia; Clinical Center “Kragujevac”, Kragujevac, Serbia
  • Zlatibor Andjelković Department oh Histology, Medical Faculty University of Pristina, Kosovska Mitrovica, Serbia
  • Miroslav Obrenović Faculty of Medicine, University of East Sarajevo, Foča, Republic of Srpska, Bosnia and Herzegovina
  • Radivoje Nikolić Department of Surgery, Faculty of Medical Sciences, University of Kragujevac, Kragujevac, Serbia; Clinical Center “Kragujevac”, Kragujevac, Serbia
DOI: https://doi.org/10.2298/4098
Ključne reči: peridontal diseases||, ||periodontalne bolesti, gingiva||, ||gingiva, histological techniques||, ||histološke tehnike, collagen||, ||kolagen, macrophages||, ||makrofagi, plasma cells||, ||plazma ćelije,

Sažetak


Uvod/Cilj. Parodontopatija utiče na tkivo desni i potpornog aparata zuba i dovodi do njegovog propadanja. Cilj ovog istraživanja bio je da se istaknu i precizno odrede histološke promene u tkivu desni. Metode. Korišćeni su uzorci biopsija gingiva kod 53 zdrave  osobe i osoba sa parodontopatijom. Izvršena je klinička gradacija oboljenja. Uzorci tkiva su fiksirani i rutinski obrađeni. Preparati debljine 5 μm bojeni su hematoksilin-eozin metodom, histohemijski po Van-Gizonu za sadržaj kolagena, Špicerovom metodom za mastocite i imunohemijski sa anti-CD68 i anti-CD38 za obeležavanje makrofaga i plazmocita. Morfometrijska analiza je izvršena upotrebom M42 test sistema. Rezultati. Dok je bolest napredovala, volumenska gustina kolagena i broj fibroblasta u teškim slučajevima smanjili su se skoro dva puta u odnosu na kontrolu, ali su uočene značajne razlike u ispitivanim grupama. Broj mastocita povećao se skoro dva puta, dok je sadržaj makrofaga bio i do tri puta veći u teškoj parodontopatiji u odnosu na zdravu gingivu. Međutim, relativni udeo tih ćelija ostao je oko 6% u svim slučajevima. Porast broja plazma ćelija bio je najizrazitiji (preko 8 puta) u odnosu na kontrolu, ali ponovo, varijacije u okviru ispitivanih grupa bile su veoma visoke. Zaključak. Destrukcija gingivalnog tkiva izazvana zapaljenskim procesom dovodi do značajnih promena u gustini kolagena i broju ćelija vezivnog tkiva. Iako inflamatorne ćelije dominiraju sa napredovanjem oboljenja, velike varijacije u okviru istih ispitivanih grupa sugerišu promenljivost patološkog procesa.

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Objavljeno
2015/07/08
Broj časopisa
Vol. 72 Br. 3 (2015)
Rubrika
Originalni članak