BCL10 aberacije i aktivacija p65 gena NF-kappa B puta su odsutne ili retke u primarnom MALT limfomu želuca

  • Jelena Hajder Clinic for Hematology and Oncology, Medical Center “Bežanijska kosa”, Belgrade, Serbia
  • Dragomir Marisavljević Clinic for Hematology and Oncology, Medical Center “Bežanijska kosa”, Belgrade, Serbia; Faculty of Medicine, University of Belgrade, Belgrade, Serbia
  • Nataša Stanisavljević Clinic for Hematology and Oncology, Medical Center “Bežanijska kosa”, Belgrade, Serbia
  • Biljana Mihaljević Faculty of Medicine, University of Belgrade, Belgrade, Serbia; Clinic for Hematology, Clinical Center of Serbia, Belgrade, Serbia
  • Vladimir Kovčin Clinic for Hematology and Oncology, Medical Center “Bežanijska kosa”, Belgrade, Serbia
  • Olivera Marković Clinic for Hematology and Oncology, Medical Center “Bežanijska kosa”, Belgrade, Serbia
  • Radmila Živković Clinic for Hematology and Oncology, Medical Center “Bežanijska kosa”, Belgrade, Serbia
DOI: https://doi.org/10.2298/2389
Ključne reči: lymphoma, b-cell, marginal zone||, ||limfom, malt, stomach neoplasms||, ||želudac, neoplazme, immunohistochemistry||, ||imunohistohemija, gene expression||, ||geni, ekspresija, signal transduction||, ||signali, transdukcija,

Sažetak


Uvod/Cilj. Limfomi limfnog tkiva pridruženog mukozi (MALT) čine 5–17% svih non-Hodgkin limfoma (NHL). Molekularna patogeneza MALT limfoma nije razjašnjena. Cilj ove studije bio je da se imunohistohemijski utvrdi prisustvo nuklearne koekspresije BCL10 i NF-kappaB (NF-κB) u tumorskim ćelijama želudačnog MALT limfoma kao i njihov uticaj na patogenezu i ishod bolesti. Metode. Analizirani su klinički podaci iz istorija bolesti 35 bolesnika sa novodijagnostikovanim želudačnim MALT limfomom i uzorci tkiva biopsije želuca bojeni su imunohistohemijskom metodom na ekspresiju BCL10 i NF-κB. Rezultati. Prosečna starost kod 35 bolesnika sa dijagnozom želudačnog MALT limfoma iznosila je 63,5 godina (muškarci/žene = 21/14). Simptomi su bili prisutni kod 23/35 (65,7%) bolesnika sa gubitkom težine kao najčešćim simptomom. Želudačni MALT limfom bio je najčešće lokalizovan u korpusu i korpusu i antrumu (45,7% i 31,2%). Infekcija H. pylori bila je potvrđena kod 20 od 30 (66,7%) bolesnika. Bolesnici su lečeni imunohemioterapijom (10 bolesnika, 28,5%), hirurški (9 bolesnika, 25,8%), kombinacijom hirurgije i hemioterapije (14 bolesnika, 40%) i suportivno (2 bolesnika, 5,7%). Kompletna remisija bila je postignuta kod 13 (37,1%) bolesnika, a parcijalna remisija kod dva (5,7%) bolesnika. Kod 16 (45,7%) bolesnika došlo je do progresije bolesti (p < 0,001). Citoplazmatska ekspresija BCL10 bila je nađena kod 19 (54,3%) tumorskih ćelija. Nuklearna ekspresija nije uočena ni u jednom uzorku. Citoplazmatska ekspresija NF-κB bila je prisutna u 22 (65,7%) uzorka, ali nuklearna ekspresija nije potvrđena ni u jednom uzorku. Zaključak. Nuklearna ekspresija (aktivacija) p65 subjedinice NF-κB puta i BCL10 nije otkrivena u uzorcima želudačnog MALT limfoma. Korelacija nuklearne koekspresije BCL10 i NF-κB u tumorskim ćelijama želudačnog MALT limfoma nije utvrđena. Ovi rezultati ukazuju da su neki drugi mehanizmi i signalni putevi aktivni u limfogenezi želudačnog MALT limfoma i da inhibicija apoptoze nije glavni i jedini mehanizam tumorogeneze.

Biografija autora

Jelena Hajder, Clinic for Hematology and Oncology, Medical Center “Bežanijska kosa”, Belgrade, Serbia
hematology

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Objavljeno
2015/04/24
Broj časopisa
Vol. 71 Br. 11 (2014)
Rubrika
Originalni članak