Različiti nivoi citokina IL-1β i IL-6 u gingivalnoj tečnosti tokom početne faze ortodontskog pomeranja zuba kod dece i mladih

  • Amila Dz. Vujačić Univerzitet u Kosovskoj Mitrovici,Medicinski Fakultet ,Odsek Stomatologija
  • Aleksandra Konić Institute for Medical Research, University of Belgrade, Belgrade, Serbia
  • Jasna Pavlović Department of Dentistry, Faculty of Medicine, University of Priština/Kosovska Mitrovica, Kosovska Mitrovica, Serbia
  • Vera Todorović Faculty of Stomatology, University of Busines Academy of Novi Sad, Pančevo, Serbia
  • Vladanka Vukičević Department of Dentistry, Faculty of Medicine, University of Priština/Kosovska Mitrovica, Kosovska Mitrovica, Serbia
  • Danimir Jevremović Faculty of Stomatology, University of Busines Academy of Novi Sad, Pančevo, Serbia
  • Nadežda Milošević-Jovčić Institute for Medical Research, University of Belgrade, Belgrade, Serbia
DOI: https://doi.org/10.2298/VSP150425178V
Ključne reči: tooth movement||, ||zub, pomeranje, child||, ||deca, adolescent||, ||adolescenti, adult||, ||odrasle osobe, interleukin-6||, ||interleukin-6, interleukin-1 beta||, ||interleukin-1 beta, gingival cervicular fluid||, ||gingivalna sulkusna tečnost, acute-phase proteins||, ||proteini akutne faze,

Sažetak


Uvod/Cilj. Postoji malo podataka o razlikama u nivou citokina u gingivalnoj tečnosti (GT) kod dece i odraslih u toku ortodontskog pomeranja zuba (OPZ). Cilj ove studije bio je da se ispitaju koncentracije citokina IL-1b i IL-6 u GT kod dece i odraslih u toku rane faze OPZ. Metode. Ispitavanje je obuhvatilo dve grupe ispitanika – 10 dece i 10 odraslih osoba, kod kojih je postavljen ortodontski separator između drugog premolara i prvog molara na jednoj strani, a suprotna strana je služila kao kontrolna. Uzorci GT uzimani su i sa lečenih i sa kontrolnih zuba i to pre, 24 h, 72 h i 168 h nakon postavljanja separatora. Nivo citokina određivan je enzyme-linked immunosorbent assay (ELISA) metodom. Rezultati. Praćenje koncentracije IL-1b i IL-6 u GT u toku rane faze OPZ pokazalo je dva pika vrednosti za oba citokina u obe grupe ispitanika – 24 h i 168 h od postavljanja separatora. Međutim, porast vrednosti IL-1b bio je statistički značajno veći kod dece nakon 168 h u GT lečenih zuba u odnosu na kontrolne, kao i u GT lečenih zuba dece u odnosu na lečene zube odraslih ispitanika. Što se tiče koncentracije IL-6 u GT, ona je kod dece bila statistički značajno viša u GT lečenih zuba o odnosu na kontrolne vrednosti kako nakon 24 h posmatranja (povećanje od oko 70 puta), tako i nakon 168 h posmatranja (povećanje od oko 55 puta). Kod odraslih se zapažao isti trend u povećanju koncentracije ovog citokina u GT lečenih zuba u odnosu na kontrolne u posmatranim periodima, ali je to povećanje, iako statistički značajno, bilo manje (oko 5 puta posle 24 h i 10 puta posle 168 h posmatranja) u odnosu na isto kod dece. Uz to, stepen pomeranja zuba izražen u mm kod dece bio je statistički viši od istog kod odraslih ispitanika nakon 168 h od primenjenog ortodontskog lečenja. Zaključak. IL-1b i IL-6 se povećano luče u GT u toku rane faze OPZ kako kod dece, tako i kod odraslih ispitanika. Međutim, sekretorni odgovor, naročito u pogledu sekrecije IL-6, daleko je veći kod dece nego kod odraslih ispitanike, što je u skladu sa nalazom da je stepen početnog pomeranja zuba brži u ovoj populaciji ispitanika.

Biografija autora

Amila Dz. Vujačić, Univerzitet u Kosovskoj Mitrovici,Medicinski Fakultet ,Odsek Stomatologija
Docent na predmetu Ortopedija vilica

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Objavljeno
2017/06/21
Broj časopisa
Vol. 74 Br. 3 (2017)
Rubrika
Originalni članak