Određivanje referentnih vrednosti aktivnosti acetil i butiril holinesteraze kod zdrave populacije u Srbiji

Milica Zlatković; Nadežda Krstić; Vesna Subota; Bogdan Bošković; Slavica Vučinić

doi:10.2298/VSP160303101Z

Milica Zlatković Military Medical Academy, National Poison Control Center, Belgrade, Serbia; University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia
Nadežda Krstić Military Medical Academy, National Poison Control Center, Belgrade, Serbia
Vesna Subota Military Medical Academy, Department of Medical Biochemistry, Belgrade, Serbia
Bogdan Bošković Military Medical Academy, National Poison Control Center, Belgrade, Serbia
Slavica Vučinić Military Medical Academy, National Poison Control Center, Belgrade, Serbia; University of Defence, Faculty of Medicine of the Military Medical Academy, Belgrade, Serbia

DOI: https://doi.org/10.2298/VSP160303101Z

Ključne reči: cholinesterases||, ||holinesteraze, butyrylcholinesterases||, ||butirilholinesteraze, cholinesterase inhibitors||, ||holinesteraza, inhibitori, insecticides||, ||insekticidi, poisoning||, ||trovanje, reference values||, ||referentne vrednosti, serbia||, ||srbija,

Sažetak

Uvod/Cilj. Acetilholinesteraza (AChE) i butirilholinesteraza (BuChe) su važni biomarkeri ekspozicije organofosfornim i karbamatnim insekticidima. S obzirom na to da procena nivoa inhibicije holinesteraze, koja odgovara težini trovanja ovim agensima, zavisi od normalnih vrednosti, koje mogu varirati u različitim populacijama, bilo je važno odrediti ih u našoj populaciji, što do sada nije urađeno. Stoga je cilj ovog rada bio da se odrede referentne vrednosti za aktivnost AChE i BuChE u zdravoj populaciji odraslih osoba na području Republike Srbije. Metode. Aktivnost AChE u eritrocitima merena je spekrofotometrijski (λ = 412 nm). Korišćena je modifikovana Ellman-ova metoda. Aktivnost BuChE u plazmi određivana je na integrisanom hemijskom sistemu (Dimenzion RxLMax) sa gotovim reagens uloškom za analizu. Ispitanici su bili dobrovoljni davaoci krvi iz Instituta za transfuziologiju i hemobiologiju Vojnomedicinske akademije. Za obradu podataka korišćen je SPSS (Statistical Package for Social Sciences) softverski program. Rezultati. U grupi od 851 osobe bilo je 728 muškaraca i 123 žene. Srednje životno doba ispitanika bilo je 39,1 ± 11,6 godina. Za sve ispitanike određena je aktivnost AChE, dok je aktivnost butirilholinesteraze određena kod 205 ispitanika (169 muškaraca i 36 žena). Srednja vrednost aktivnosti acetilholinesteraze iznosila je 8 090,6 ± 1 976,7 IJ/L, a za butirilholinesterazu 14 556.6 ± 4 078,1 U/L. Odsustvo normalne distribucije podataka kod muškaraca uslovilo je da referentne vrednosti enzima odredimo kao 2,5-ti i 97,5-ti percentil. Zaključak. Rezultati ove pilot studije o aktivnosti holinesteraza u zdravoj populaciji u Republici Srbiji, koja je sada urađena prvi put, ukazuju na potrebu da se pri proceni težine trovanja uzmu u obzir širi rasponi aktivnosti holinesteraza. Preporučuje se proširivanje studije za BuChE, sa uključenjem većeg broja ispitanika ženskog pola i podataka o telesnoj masi, u cilju dobijanja preciznijih referentnih vrednosti.

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