Antigenotoxic properties of anthocyanin-enriched fraction of strawberry (cv. Romina) extract on DNA damage induced by H2O2 in human peripheral blood leukocytes

Biljana Spremo-Potparević; Andrea Čabarkapa-Pirković; Dragana Dekanski; Dijana Topalović; Vladan Bajić; Lada Živković

doi:10.5937/arhfarm71-31060

Biljana Spremo-Potparević University of Belgrade - Faculty of Pharmacy, Center of Biological Research
Andrea Čabarkapa-Pirković University of Belgrade - Faculty of Pharmacy, Center of Biological Research
Dragana Dekanski University of Belgrade - Institute for Application of Nuclear Energy-INEP, Department for Biology of Reproduction
Dijana Topalović University of Belgrade - Faculty of Pharmacy, Center of Biological Research
Vladan Bajić University of Belgrade - Institute of Nuclear Sciences Vinča, Laboratory for Radiobiology and Molecular Genetics
Lada Živković

DOI: https://doi.org/10.5937/arhfarm71-31060

Keywords: DNA damage, comet assay, strawberry (cv. Romina), anthocyanins, leukocytes

Abstract

Strawberry fruit Fragaria × ananassa Duchesne, Rosaceae (cv. Romina), rich in anthocyanin polyphenols, has been demonstrated to have favorable effects on health due to its antioxidant properties. The present study investigated the antigenotoxic potential of anthocyanin-enriched fraction of Romina strawberry methanolic extract (ACY) against DNA damage on human peripheral blood leukocytes, induced by hydrogen peroxide in vitro. Five concentrations of the ACY extract were used in all experiments (2.5, 5, 10, 15, 20 µg/ml). The results of the alkaline comet assay showed no genotoxic effect of the ACY. After the pre-exposure of the leukocytes to the ACY, and subsequent incubation with H₂O₂, a decreased number of DNA damaged cells was recorded in all the tested concentrations, compared to controls. In the post-treatment, there was a concentration-dependent DNA damage reduction, while a statistically significant decrease was achieved with 15 and 20 µg/ml concentrations. The results indicate that ACY is efficient in oxidative DNA damage reduction, and it is more potent as a post-applicative than a protective agent.

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