Metode određivanja antioksidativne aktivnosti biljnih ekstrakata in vitro

  • Jelena Kukić-Marković Univerzitet u Beogradu – Farmaceutski fakultet, Katedra za farmakognoziju
DOI: https://doi.org/10.5937/arhfarm73-38573
Ključne reči: antioksidansi, biljni ekstrakti, in vitro testovi

Sažetak


Biljke su bogati izvori sekundarnih metabolita koji ispoljavaju raznovrsne biološke i farmakološke efekte. Neki biljni sastojci, u prvom redu polifenolna jedinjenja, ispoljavaju značajnu in vitro antioksidativnu aktivnost, zbog čega se smatra da mogu doprineti održanju redoks ravnoteže u organizmu. Ovi potencijalni antioksidativni agensi strukturno su veoma raznovrsni deluju različitim antioksidantnim mehanizmima. S obzirom na rastuću potrebu za iznalaženjem, razvojem i razumevanjem efikasnih antioksidanasa, interesovanje za ispitivanje antioksidanasa u različitim biljnim izolatima kontinuirano raste, pa su shodno tome i razvijeni mnogi testovi. Većina dostupnih in vitro testova je pristupačna i jednostavna za izvođenje, ali zbog složenog sastava biljnih ekstrakata, različite kinetike, mehanizama i specifičnosti hemijskih reakcija na kojima se ovi testovi zasnivaju, još uvek ne postoji univerzalni parametar za procenu antioksidativne aktivnosti. U ovom radu su prikazane neke od trenutno najkorišćenijih in vitro metoda za ispitivanje i procenu antioksidativne aktivnosti biljnih ekstrakata, s naglaskom na njihove prednosti i nedostatke.

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Objavljeno
2023/08/30
Broj časopisa
Vol. 73 Br. Notebook 4 (2023): Arhiv za farmaciju
Rubrika
Pregledni (Revijalni) rad
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